From field to plate: How do bacterial enteric pathogens interact with ready-to-eat fruit and vegetables, causing disease outbreaks?

Thomas, Gareth A.; Paradell Gil, Teresa; Müller, Carsten T.; Rogers, Hilary J.; Berger, Cedric N.

doi:10.1016/j.fm.2023.104389

Cited by 23 publications

(15 citation statements)

References 225 publications

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“…A more profound effect was observed when the final bacterial concentration was determined in spiked RTE salad samples, as detailed in Section 2.2.3 , where roughly a 2 log CFU/g reduction was observed, as shown in Figure 3 B. These results are reasonable, considering the high concentrations of interfering bacteria expected in this type of food product, where up to 7 log CFU/g were previously reported [ 22 , 50 , 51 ]. Of the utmost importance is the fact that all three pathogens reached concentrations higher than 5 log CFU/g and returned positive results via multiplex qPCR.…”

Section: Discussionsupporting

confidence: 80%

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Evaluation of the Novel mTA10 Selective Broth, MSB, for the Co-Enrichment and Detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes in Ready-to-Eat Salad Samples

Costa-Ribeiro,

Lamas,

Prado

et al. 2023

Foods

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Multiplex assays implementing DNA-based methods have been demonstrated as suitable alternatives to culture-based microbiological methods; however, in most cases, they still require a suitable enrichment step. Finding suitable enrichment conditions for different bacteria may result in challenges. In the present study, a novel selective broth named MSB (mTA10 selective broth) was formulated for the simultaneous recovery of Salmonella spp., E. coli O157:H7 and L. monocytogenes. Attention was paid to ensure the optimal enrichment of L. monocytogenes as its enrichment is more challenging. To this end, cellobiose was added to increase the growth of L. monocytogenes, and sodium pyruvate was also added to improve the recovery of stressed bacteria. Four selective agents were added, namely nalidixic acid, sodium cholate, lithium chloride and potassium tellurite, to control the growth of interfering microorganisms. It was concluded that the novel broth was suitable for the simultaneous enrichment of the target pathogens, allowing them to reach concentrations higher than 7 log CFU/mL for each bacterium in pure culture. Furthermore, all heavily contaminated ready-to-eat salad samples reached concentrations higher than 5 log CFU/g. Finally, after 24 h of enrichment of spiked salad, it was possible to detect concentrations below 10 CFU/25 g.

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Section: Discussionsupporting

confidence: 80%

“…RTE salad samples are a popular way of vegetable intake and have also been identified as carriers of different pathogens [ 22 , 50 , 51 ]. This type of product has been reported to contain high concentrations of mesophilic bacteria [ 21 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Novel mTA10 Selective Broth, MSB, for the Co-Enrichment and Detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes in Ready-to-Eat Salad Samples

Costa-Ribeiro,

Lamas,

Prado

et al. 2023

Foods

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“…Due to the pathogens it carries, fecal contamination is source of several infectious conditions which could sometimes be fatal. This includes gastrointestinal infections, hepatitis, respiratory infections, and skin rashes [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Microbial and heavy metal contamination in herbal medicine: a prospective study in the central region of Saudi Arabia

Alharbi,

Althbah,

Mohammed

et al. 2024

BMC Complement Med Ther

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Introduction Herbal medicine is a medical system based on the utilization of plants or plant extracts for therapy. The continual increase in global consumption and the trade of herbal medicine has raised safety concerns in many regions. These concerns are mainly linked to microbial contamination, which could spread infections with multi-resistant bacteria in the community, and heavy metal contamination that may lead to cancers or internal organs’ toxicity. Methods This study was performed using an experimental design. A total of 47 samples, herbal medicine products sold in local stores in Qassim region, were used in the experiments. They were tested for bacterial contamination, alongside 32 samples for heavy metal analysis. Bacterial contamination was determined by the streak plate method and further processed to determine their antimicrobial susceptibility patterns using MicroScan WalkAway96 pulse; heavy metals were determined using a spectrometer instrument. Results A total of 58 microorganisms were isolated. All samples were found to be contaminated with at least one organism except three samples. The majority of the isolated bacterial species were gram negative bacteria, such as Klebsiella spp., Pseudomonas spp. and E. coli., which could be of fecal origin and may lead to pneumonia, skin, or internal infections. Furthermore, most of the gram-positive bacteria were found to be multi-drug resistant. Moreover, for heavy metals, all samples had levels exceeding the regulatory limits. Conclusion This study demonstrated the presence of bacteria and heavy metals in samples of herbal medicines. Using these contaminated products may spread resistant infections, metal toxicities, or even cancers in the community.

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“…It has been suggested by a study of major pathogens in the U.S. that 39% of foodborne illnesses and 64% of the resulting deaths can be ascribed to bacterial origins . It has been further suggested that cross-contamination of food in the supply chain can be a contributing factor in large scale illness outbreaks. , As many as 29% of all food safety loss incidents are the result of microbiological pathogen cross-contamination of food preparation surfaces …”

Section: Introductionmentioning

confidence: 99%

Bioinspired Superhydrophobic Nanocoating Based on Polydopamine and Nanodiamonds to Mitigate Bacterial Attachment to Polyvinyl Chloride Surfaces in Food Industry Environments

DeFlorio,

Zaza,

Arcot

et al. 2024

Ind. Eng. Chem. Res.

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Polyvinyl chloride (PVC) is commonly utilized as a food-contact surface by the food industry for processing and storage purposes due to its durability, ease of fabrication, and cost-effectiveness. Herein, we report a composite coating for the superhydrophobization of PVC without the use of polyfluoroalkyl chemistry. This coating rendered the PVC superhydrophobic, exhibiting a static water contact angle of 151.9 ± 0.7° and a contact angle hysteresis of only 3.1 ± 1.0°. The structure of this composite coating, consisting of polydopamine, nanodiamonds, and an alkyl silane, was investigated by utilizing both scanning electron microscopy and atomic force microscopy. Surface chemistry was probed using attenuated total reflectance-Fourier transform infrared, and the surface wetting behavior was thoroughly characterized using both static and dynamic water contact angle measurements. It was demonstrated that the superhydrophobic PVC was cleanable using a food-grade surfactant, becoming wet in contact with high concentration surfactant solutions, but regaining its nonwetting property upon rinsing with water. It was demonstrated that the coating produced a 2.1 ± 0.1 log10 reduction (99.2%) in the number of Escherichia coli O157:H7 cells and a 2.2 ± 0.1 log10 reduction (99.3%) in the number of Salmonella enterica Typhimurium cells that were able to adsorb onto PVC surfaces over a 24 h period. The use of this fluorine-free superhydrophobic coating on PVC equipment, such as conveyor belts within food production facilities, may help to mitigate bacterial cross-contamination and curb the spread of foodborne illnesses.

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From field to plate: How do bacterial enteric pathogens interact with ready-to-eat fruit and vegetables, causing disease outbreaks?

Cited by 23 publications

References 225 publications

Evaluation of the Novel mTA10 Selective Broth, MSB, for the Co-Enrichment and Detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes in Ready-to-Eat Salad Samples

Evaluation of the Novel mTA10 Selective Broth, MSB, for the Co-Enrichment and Detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes in Ready-to-Eat Salad Samples

Microbial and heavy metal contamination in herbal medicine: a prospective study in the central region of Saudi Arabia

Bioinspired Superhydrophobic Nanocoating Based on Polydopamine and Nanodiamonds to Mitigate Bacterial Attachment to Polyvinyl Chloride Surfaces in Food Industry Environments

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