From freezing to functioning: cellular strategies of cold-adapted bacteria for surviving in extreme environments

Marine bacteria that have adapted to thrive in extreme environments, such as Pseudoalteromonas haloplanktis TAC125 (PhTAC125), offer a unique biotechnological potential. The discovery of an endogenous megaplasmid (pMEGA) raised questions about its metabolic impact and functional role in this strain. This study aimed at streamlining the host genetic background by curing PhTAC125 from the pMEGA plasmid using a sequential genetic approach. We combined homologous recombination by exploiting a suicide vector with the PTasRNA gene silencing technology to interfere with pMEGA replication machinery. This approach led to the construction of the novel PhTAC125 KrPL2 strain, cured from the pMEGA plasmid, which exhibited no significant differences in the growth behaviour, though showcasing enhanced resistance to oxidative stress and a reduced capability of biofilm formation. These findings represent a significant achievement for understanding of the role of pMEGA plasmid and for the biotechnological applications of PhTAC125 in recombinant protein production. This opens up the possibility to exploit pMEGA valuable genetic elements and further advancing the genetic tools for PhTAC125.

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Changes in Climate and Their Implications for Cattle Nutrition and Management

Muzzo,

Ramsey,

Villalba

2024

Climate

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Climate change is a global challenge that impacts rangeland and pastureland landscapes by inducing shifts in temperature variability, precipitation patterns, and extreme weather events. These changes alter soil and plant conditions, reducing forage availability and chemical composition and leading to nutritional stress in cattle. This stress occurs when animals lack adequate water and feed sources or when these resources are insufficient in quantity, composition, or nutrient balance. Several strategies are essential to address these impacts. Genetic selection, epigenetic biomarkers, and exploration of epigenetic memories present promising avenues for enhancing the resilience of cattle populations and improving adaptation to environmental stresses. Remote sensing and GIS technologies assist in locating wet spots to establish islands of plant diversity and high forage quality for grazing amid ongoing climate change challenges. Establishing islands of functional plant diversity improves forage quality, reduces carbon and nitrogen footprints, and provides essential nutrients and bioactives, thus enhancing cattle health, welfare, and productivity. Real-time GPS collars coupled with accelerometers provide detailed data on cattle movement and activity, aiding livestock nutrition management while mitigating heat stress. Integrating these strategies may offer significant advantages to animals facing a changing world while securing the future of livestock production and the global food system.

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From freezing to functioning: cellular strategies of cold-adapted bacteria for surviving in extreme environments

Cited by 4 publications

References 89 publications

Integrating physiological and molecular insights in cotton under cold stress conditions

Integrating physiological and molecular insights in cotton under cold stress conditions

Engineering the MarinePseudoalteromonas haloplanktisTAC125 via pMEGA Plasmid Targeted Curing Using PTasRNA Technology

Changes in Climate and Their Implications for Cattle Nutrition and Management

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