From fundamentals to applications of bioelectrocatalysis: bioelectrocatalytic reactions of FAD-dependent glucose dehydrogenase and bilirubin oxidase

Abstract

In this review, I present the main highlights of my works in the development of bioelectrocatalysis, which can be used in widespread applications, particularly for the design of biosensor and biofuel cells. In particular, I focus on research progress made in two key bioelectrocatalytic reactions: glucose oxidation by flavin adenine dinucleotide-dependent glucose dehydrogenase and oxygen reduction by bilirubin oxidase. I demonstrate the fundamental principles of bioelectrocatalysis and the requirements for enhancing the catalytic performance, including the choice of a mediator of redox reactions, immobilization, and electrode materials. These methods can allow for achieving control of the bioelectrocatalytic reaction, thereby overcoming obstacles toward their industrial applications.

Stretchable Liquid Metal-Based Metal-Polymer Conductors for Fully Screen-Printed Biofuel Cells

“…To functionalize the electrode, we prepared 40 mg/mL lactate oxidase (LOx), 40 mg/mL bilirubin oxidase (BOx), and 40 mg/mL glucose oxidase (GOD), respectively. For biocathode, BOx can reduce oxygen under physiological conditions and realize direct electron transfer, so it is used as a cathodic material for biosensors . PPIX is the mediator of bilirubin oxidase for electron transferring, so it was added before loading BOx.…”

Porous carbon‐based electrochemical platform for direct electron transfer in myoglobin

“…Carbon materials such as nanostructured porous material are promising for the high performance of oxidoreductase-based electrodes for the design of biosensors and biofuel cells [33][34][35][36]. The high specific surface area of nanomaterial exhibits an effective surface area and is the reason behind the excellent response of the biosensor.…”

Glucose Sensor Strip Using Flavin Adenine Dinucleotide-dependent Glucose Dehydrogenase with Quinones as Redox Mediators

“…Recently, flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase (FAD-GDH) has been widely used as a glucose-oxidizing enzyme instead of glucose oxidase (GOx). (5)(6)(7)(8)(9) GOx uses O 2 as an electron acceptor, which can lead to measurement errors depending on the blood O 2 concentration. However, FAD-GDH does not employ O 2 as an electron acceptor.…”