2014
DOI: 10.1007/978-1-4939-0446-4_2
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From Genes to Markers: Exploiting Gene Sequence Information to Develop Tools for Plant Breeding

Abstract: Once the sequence is known for a gene of interest, it is usually possible to design markers to detect polymorphisms within the gene. Such markers can be particularly useful in plant breeding, especially if they detect the causal polymorphism within the gene and are diagnostic of the phenotype. In this chapter, we (1) discuss how gene sequences are obtained and aligned and how polymorphic sites can be identified or predicted; (2) explain the principles of PCR primer design and PCR amplification and provide guid… Show more

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Cited by 4 publications
(2 citation statements)
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“…In contrast, CAPS markers can be developed to study length polymorphisms using restriction enzymes specific to the polymorphic nucleotides. Since even a single nucleotide can create or destroy the restriction sites, it leads to restriction fragment length polymorphism (Garcia & Mather, 2014). For other length polymorphisms, obtained from InDels, SSRs including CAPS, can be detected using agarose gel electrophoresis, poly-acrylamide gel electrophoresis, and capillary separation.…”
Section: Development Of Cost Effective Allele Specific Fmmsmentioning
confidence: 99%
“…In contrast, CAPS markers can be developed to study length polymorphisms using restriction enzymes specific to the polymorphic nucleotides. Since even a single nucleotide can create or destroy the restriction sites, it leads to restriction fragment length polymorphism (Garcia & Mather, 2014). For other length polymorphisms, obtained from InDels, SSRs including CAPS, can be detected using agarose gel electrophoresis, poly-acrylamide gel electrophoresis, and capillary separation.…”
Section: Development Of Cost Effective Allele Specific Fmmsmentioning
confidence: 99%
“…Moreover, MAS is usually done during the early stages of plant growth cycles and only those plants with positive markers are kept for further phenotyping which saves resources. Multiplex marker assays allow the simultaneous analysis of multiple markers for different traits which increases MAS efficiency [ 29 ].…”
Section: Introductionmentioning
confidence: 99%