From genome to phenome: genome‐wide association studies and other approaches that bridge the genotype to phenotype gap

Fernie, Alisdair R.; Gutierrez-Marcos, José

doi:10.1111/tpj.14219

Cited by 24 publications

(16 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only 30 years ago, we entered the third generation of breeding (3G), which is also known as the second green revolution or biotechnology-based breeding. This generation involves the application of transgenic technology and the utilization of genome breeding technology, with genome-wide association studies (Risch and Merikangas, 1996;Fernie and Gutierrez-Marcos, 2019), marker-assisted breeding (Lander and Botstein, 1989) and genomic selection (Meuwissen et al, 2001) harnessed for crop improvement. Currently we are at the start of the fourth generation of breeding (4G), which promises to be the third green revolution, and involves design breeding ( Figure 2).…”

Section: The History Of Crop Domestication and Improvementmentioning

confidence: 99%

De Novo Domestication: An Alternative Route toward New Crops for the Future

2019

Self Cite

View full text Add to dashboard Cite

Current global agricultural production must feed over 7 billion people. However, productivity varies greatly across the globe and is under threat from both increased competitions for land and climate change and associated environmental deterioration. Moreover, the increase in human population size and dietary changes are putting an ever greater burden on agriculture. The majority of this burden is met by the cultivation of a very small number of species, largely in locations that differ from their origin of domestication. Recent technological advances have raised the possibility of de novo domestication of wild plants as a viable solution for designing ideal crops while maintaining food security and a more sustainable lowinput agriculture. Here we discuss how the discovery of multiple key domestication genes alongside the development of technologies for accurate manipulation of several target genes simultaneously renders de novo domestication a route toward crops for the future.

show abstract

Section: The History Of Crop Domestication and Improvementmentioning

confidence: 99%

De Novo Domestication: An Alternative Route toward New Crops for the Future

2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Significant crop-yield-improvement potential has contributed immensely to a rising food demand over the past few decades, consistently keeping pace with rising global demand. The history of crop improvements has been a progressive one, from first- to fourth-generation breeding [ 191 , 192 , 193 , 194 , 195 ]. In the 21st century, the world operates in the fourth generation of breeding.…”

Section: Integrated Approaches To Crop-yield Improvementmentioning

confidence: 99%

Sucrose Utilization for Improved Crop Yields: A Review Article

Aluko

Wang

et al. 2021

IJMS

104

View full text Add to dashboard Cite

Photosynthetic carbon converted to sucrose is vital for plant growth. Sucrose acts as a signaling molecule and a primary energy source that coordinates the source and sink development. Alteration in source–sink balance halts the physiological and developmental processes of plants, since plant growth is mostly triggered when the primary assimilates in the source leaf balance with the metabolic needs of the heterotrophic sinks. To measure up with the sink organ’s metabolic needs, the improvement of photosynthetic carbon to synthesis sucrose, its remobilization, and utilization at the sink level becomes imperative. However, environmental cues that influence sucrose balance within these plant organs, limiting positive yield prospects, have also been a rising issue over the past few decades. Thus, this review discusses strategies to improve photosynthetic carbon assimilation, the pathways actively involved in the transport of sucrose from source to sink organs, and their utilization at the sink organ. We further emphasize the impact of various environmental cues on sucrose transport and utilization, and the strategic yield improvement approaches under such conditions.

show abstract

“…Genome‐wide association studies (GWAS) are becoming increasingly important in crop breeding because they connect genotypes with phenotypes (Fernie and Gutierrez‐Marcos, 2019). In cassava, GWAS of 672 cassava clones and 72 000 single nucleotide polymorphism loci used the yellow color intensity (yellowness) of storage roots to indirectly assess variation in carotenoid and dry matter content (Rabbi et al ., 2017).…”

Section: Learning From Genetic Diversity Of Cassavamentioning

confidence: 99%

The Cassava Source–Sink project: opportunities and challenges for crop improvement by metabolic engineering

et al. 2020

Self Cite

View full text Add to dashboard Cite

Summary Cassava (Manihot esculenta Crantz) is one of the important staple foods in Sub‐Saharan Africa. It produces starchy storage roots that provide food and income for several hundred million people, mainly in tropical agriculture zones. Increasing cassava storage root and starch yield is one of the major breeding targets with respect to securing the future food supply for the growing population of Sub‐Saharan Africa. The Cassava Source–Sink (CASS) project aims to increase cassava storage root and starch yield by strategically integrating approaches from different disciplines. We present our perspective and progress on cassava as an applied research organism and provide insight into the CASS strategy, which can serve as a blueprint for the improvement of other root and tuber crops. Extensive profiling of different field‐grown cassava genotypes generates information for leaf, phloem, and root metabolic and physiological processes that are relevant for biotechnological improvements. A multi‐national pipeline for genetic engineering of cassava plants covers all steps from gene discovery, cloning, transformation, molecular and biochemical characterization, confined field trials, and phenotyping of the seasonal dynamics of shoot traits under field conditions. Together, the CASS project generates comprehensive data to facilitate conventional breeding strategies for high‐yielding cassava genotypes. It also builds the foundation for genome‐scale metabolic modelling aiming to predict targets and bottlenecks in metabolic pathways. This information is used to engineer cassava genotypes with improved source–sink relations and increased yield potential.

show abstract

From genome to phenome: genome‐wide association studies and other approaches that bridge the genotype to phenotype gap

Cited by 24 publications

References 28 publications

De Novo Domestication: An Alternative Route toward New Crops for the Future

De Novo Domestication: An Alternative Route toward New Crops for the Future

Sucrose Utilization for Improved Crop Yields: A Review Article

The Cassava Source–Sink project: opportunities and challenges for crop improvement by metabolic engineering

Contact Info

Product

Resources

About