From hair to liver: emerging application of hair follicle mesenchymal stem cell transplantation reverses liver cirrhosis by blocking the TGF-β/Smad signaling pathway to inhibit pathological HSC activation

Abstract

Liver cirrhosis (LC) involves multiple systems throughout the body, and patients with LC often die of multiple organ failure. However, few drugs are useful to treat LC. Hair follicle mesenchymal stem cells (HF-MSCs) are derived from the dermal papilla and the bulge area of hair follicles and are pluripotent stem cells in the mesoderm with broad prospects in regenerative medicine. As an emerging seed cell type widely used in skin wound healing and plastic surgery, HF-MSCs show considerable prospects in the treatment of LC due to their proliferation and multidirectional differentiation capabilities. We established an LC model in C57BL/6J mice by administering carbon tetrachloride (CCl4) and injected HF-MSCs through the tail vein to explore the therapeutic effects and potential mechanisms of HF-MSCs on LC. Here, we found that HF-MSCs improved liver function and ameliorated the liver pathology of LC. Notably, PKH67-labeled HF-MSCs were detected in the injured liver and expressed the hepatocyte-specific markers cytokeratin 18 (CK18) and albumin (ALB). In addition, in contrast to that in the LC group, the α-SMA expression showed a decreasing trend in the treatment group in vitro and in vivo, indicating that the pathological activation of hepatic stellate cells (HSCs) was inhibited by HF-MSC treatment. Moreover, the levels of transforming growth factor β (TGF-β1) and p-Smad3, a signaling molecule downstream of TGF-β1, were increased in mice with LC, while HF-MSC treatment reversed these changes in vivo and in vitro. Based on these findings, HF-MSCs may reverse LC by blocking the TGF-β/Smad pathway and inhibiting the pathological activation of HSCs, which may provide evidence for the application of HF-MSCs to treat LC.

BMSCs alleviate liver cirrhosis by regulating Fstl1/Wnt/β-Catenin signaling pathway

“…Previous studies have found that pluripotent stem cells, such as bone marrow mesenchymal stem cells [ 21 , 22 ], adipose mesenchymal stem cells [ 23 ], and hair follicle stem [ 24 ], can alleviate the progression of cirrhosis. In this study, we selected the most accessible and effective cells, BMSCs, for the treatment of liver cirrhosis and studied their mechanism.…”

Catching Them Early: Framework Parameters and Progress for Prenatal and Childhood Application of Advanced Therapies

“…For cancer treatment, MSCs may show microenvironment-dependent pro-or anti-tumorigenic properties when unmodified, but clearly assume therapeutic properties when used for the exosomal delivery of cytotoxic agents after drug loading or after genetic manipulation [72,73]. In the treatment of heart, liver or kidney failure, MSCs variably mediate the stimulation of endothelial cells [74], inhibit apoptosis, inflammation and hepatic stellate cell activation [75,76], and deliver trophic factors and cell components by paracrine or exosome signaling and even cell fusion [77], respectively, in order to achieve therapeutic action.…”