From image analysis to chemical analysis of bacteria: A long-term study?

Psenner, Roland

doi:10.4319/lo.1990.35.1.0234

Cited by 28 publications

(13 citation statements)

References 6 publications

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“…4, Table 3) is significantly higher than 1, which means that the C:volume ratio is lower for the small bacteria than for the larger ones. This result apparently contradicts previous related studies (Lee & Fuhrman 1987, Psenner 1990). It should, however, be noted that the among-sample variation for C:volurne ratios is high (CV = 0.38; Table 3), and the number of samples is low.…”

Section: Carbon-to-volume Ratiocontrasting

confidence: 56%

Content of carbon, nitrogen, oxygen, sulfur and phosphorus in native aquatic and cultured bacteria

Km¹,

Heldal²,

Norland³

1996

Aquat. Microb. Ecol.

541

429

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The content of carbon, nitrogen, oxygen, phosphorus and sulfur was measured in individual cells from 6 native aquatic samples and 4 samples of cultured bacteria by X-ray microanalysis using a transmission electron microscope (TEM). The molar C N:P ratio for the pooled sample was 50:lO:l From length and width measurements of unfixed air-dried cells we estimated cell volumes over a total range of 0.0026 to 15.8 pm3. and mean C:volume ratios of 30 to 162 fg for the samples included. For the marine samples we found mean N:C ratios of 0.25 to 0.28, while cells from fresh or brackish waters had mean N:C ratios of 0.17 to 0.20, indicating differences in nutrient availability. The P:C ratios for the samples analyzed varied from 0.040 to 0.090, with a pooled mean of 0.052, which is approximately twice that of the Redfield ratio for P C . For 0:C ratios we estimated a pooled mean of 0.37 and a range of 0.22 to 0.77 for all samples. We may conclude that slow-growing or non-growing cells have low 0 -C ratios. The mean S:C ratio for all samples was 0.031, with a range of 0 016 to 0.084 for the sample means. A general conclus~on is that single-cell analyses of elemental composition give important information on the physiological conditions of cells and on possible nutrient limitations. The rationale for this is the assumption that changes in macromolecular composition are due to nutrient availability.

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Section: Carbon-to-volume Ratiocontrasting

confidence: 56%

Content of carbon, nitrogen, oxygen, sulfur and phosphorus in native aquatic and cultured bacteria

Km¹,

Heldal²,

Norland³

1996

Aquat. Microb. Ecol.

541

429

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“…Joint & Pomroy 1987, Suzuki et al 1993). Major concerns are directed to the discrepancies among the results obtained via different techniques (Psenner 1990). Intercomparisons among operators and methods have been attempted by a group of scientists; differences were mostly due to the different sizing criteria (M. Sieracki pers.…”

Section: Methodsmentioning

confidence: 99%

Imperfect retention of natural bacterioplankton cells by glass fiber filters

Lee¹,

Kang²,

Fuhrman³

1995

Mar. Ecol. Prog. Ser.

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Glass fiber filters are widely used to concentrate and collect a variety of particles suspended in seawater. The filters are particularly useful for chemical analyses of the filter-retained particles, because of the physico-chemical stabihty of the matenal the filters are made of. Despite this usefulness, very small particles, e.g. many planktonic bacteria, are known to pass the glass fiber filters, and little information is currently available on the fraction passing the filters. In this study, natural bacterioplankton cells were filtered through Whatman GF/F filters, and apparent cell size-frequency distributions were obtained by epifluorescence photomicrography before and after the filtration. Seawater samples were from a northwest Atlantic coast and an Antarctic sea. Filtrate contained 35 to 43 % of the total bacterial cell count, equivalent to 22 to 38 % of the total bacterial biomass. Size-frequency distributions showed that cells larger than 0.8 pm diameter (volume-equivalent spherical diameter, VESD) did not pass the filters, however the filters retained substantial numbers of small (VESD <0.8 pm) bacteria. In comparisons of the different types of glass fiber filters, retention efficiency (fraction of the cells retained by a filter) ranged from ca 13 to 51 %, i.e. 49 to 87 % of the natural bacterioplankton cells passed through the tested filters. Interpretations of data obtained via filtrations through glass fiber filters must properly consider the fraction not retained by the filters.

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“…Bacterial biovolume was converted to carbon with a very conservative factor of 120 fg C pm-? that corresponds to a content of 6 fg C for a 0.05-km-3 cell, the lowest prediction of the different carbon-to-volume volume-dependent conversion factors for bacteria (discused in Psenner 1990). Microzooplankton wet weight was converted to carbon with a factor of 0.1 (Borsheim and Bratbak 1987), and zooplankton wet weight to carbon with a factor of 0.068 (Bamstedt 1986).…”

Section: Methodsmentioning

confidence: 99%

Biomass distribution in marine planktonic communities

Gasol

Giorgio

Duarte

1997

Limnology & Oceanography

342

255

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Patterns in primary production and carbon export from the cuphotic zone suggest that the relative contribution of planktonic heterotrophs to community biomass should decline along gradients of phytoplankton biomass and primary production. Here, we use an extensive literature data survey to test the hypothesis that the ratio of total heterotrophic (bacteria + protozoa + mesozooplankton) biomass to total autotrophic biomass (H : A ratio) is not constant in marine plankton communities but rather tends to decline with increasing phytoplankton biomass and primary production. Our results show that the plankton of unproductive regions are characterized by very high relative heterotrophic biomasses resulting in inverted biomass pyramids, whereas the plankton of productive areas are characterized by a smaller contribution of heterotrophs to community biomass and a normal biomass pyramid with a broad autotrophic base. Moreover, open-ocean communities support significantly more heterotrophic biomass in the upper layers than do coastal communities for a given autotrophic biomass. These differences in the biomass structure of the community could be explained by the changes in the biomass-specific rates of phytoplankton production that seem to occur from ultraoligotrophic to eutrophic marine regions, but other factors could also generate them. The patterns described suggest a rather systematic shift from consumer control of primary production and phytoplankton biomass in open ocean to resource control in upwelling and coastal areas.

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From image analysis to chemical analysis of bacteria: A long-term study?

Cited by 28 publications

References 6 publications

Content of carbon, nitrogen, oxygen, sulfur and phosphorus in native aquatic and cultured bacteria

Content of carbon, nitrogen, oxygen, sulfur and phosphorus in native aquatic and cultured bacteria

Imperfect retention of natural bacterioplankton cells by glass fiber filters

Biomass distribution in marine planktonic communities

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