From Moderately Severe to Severe Hypertriglyceridemia Induced Acute Pancreatitis: Circulating MiRNAs Play Role as Potential Biomarkers

An, Fangmei; Zhan, Qiang; Xia, Min; Jiang, Lisha; Lu, Guoming; Huang, Mindan; Guo, Ji-zhong; Liu, Side

doi:10.1371/journal.pone.0111058

Cited by 36 publications

(25 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, a few studies have examined circulating miRNAs in patients with pancreatitis and have sought to identify miRNAs that are predictive for the diagnosis or prognosis of pancreatitis3536. However, there is no study about the potential biomarkers for the early diagnosis of CP.…”

Section: Discussionmentioning

confidence: 99%

Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis

Xin

Gao

Wang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Chronic pancreatitis (CP) is an inflammatory disease characterized by progressive fibrosis of pancreas. Early diagnosis will improve the prognosis of patients. This study aimed to obtain serum miRNA biomarkers for early diagnosis of CP. In the current study, we analyzed the differentially expressed miRNAs (DEmiRs) of CP patients from Gene Expression Omnibus (GEO), and the DEmiRs in plasma of early CP patients (n = 10) from clinic by miRNA microarrays. Expression levels of DEmiRs were further tested in clinical samples including early CP patients (n = 20), late CP patients (n = 20) and healthy controls (n = 18). The primary endpoints were area under curve (AUC) and expression levels of DEmiRs. Four DEmiRs (hsa-miR-320a-d) were obtained from GEO CP, meanwhile two (hsa-miR-221 and hsa-miR-130a) were identified as distinct biomarkers of early CP by miRNA microarrays. When applied on clinical serum samples, hsa-miR-320a-d were accurate in predicting late CP, while hsa-miR-221 and hsa-miR-130a were accurate in predicting early CP with AUC of 100.0% and 87.5%. Our study indicates that miRNA expression profile is different in early and late CP. Hsa-miR-221 and hsa-miR-130a are biomarkers of early CP, and the panel of the above 6 serum miRNAs has the potential to be applied clinically for early diagnosis of CP.

show abstract

Section: Discussionmentioning

confidence: 99%

Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis

Xin

Gao

Wang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Kuśnierz-Cabala B et al demonstrated that the expression levels of miR-126-5p, miR-148a-3p, miR-216a-5p, miR-551b-5p and miR-375 were significantly elevated in moderate and severe acute pancreatitis (SAP) when compared to control subjects; however, in mild acute pancreatitis (MAP) patients, only miR-216a-5p, miR-551b-5p, and miR-375 were highly expressed [13]. Ma X et al reported that miR-21 was overexpressed in a murine model of AP [14], and An F et al discovered that miR-24-3p, miR-361-5p, miR-1246 and miR-222-3p were constantly up-regulated, and miR-181a-5p was constantly down-regulated, in hypertriglyceridemia-induced acute pancreatitis patients [15]. Moreover, it has been reported that miR-375, miR-217, miR-148a, miR-216a, miR-122, miR-214 and miR-138 were increased in mesenteric lymph nodes from rats with AP, and miR-217, miR-375, miR-122 and miR-148a were also increased in matched rat plasma samples [16].…”

Section: Introductionmentioning

confidence: 99%

miR-155-5p is Negatively Associated with Acute Pancreatitis and Inversely Regulates Pancreatic Acinar Cell Progression by Targeting Rela and Traf3

Liu¹,

Zou²,

Wang³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Acute pancreatitis contributes to high mortality in pancreatitis patients, and miRNAs play a vital role in the development of acute pancreatitis (AP), however, its precise biological role remains largely elusive. Methods: To clarify the potential mechanisms of miRNAs in AP, we built mouse models of mild acute pancreatitis (MAP) and moderate/ severe acute pancreatitis (SAP). MiRNA microarray analysis and Real-time quantitative PCR (qRT-PCR) were used to analyze the expression of miRNA in MAP/SAP. TargetScan software, dual-luciferase gene reporter assays and Western blotting were used to assess the target genes of miR-155-5p in AP. Results: miR-155-5p was significantly decreased in MAP/SAP mice compared to controls. In pancreatic acinar AR42J cells transfected with miR-155-5p mimic, the expression of Rela and Traf3 notably decreased in both the caerulein- and TLC-S-induced groups compared with the negative control (NC); however, the expression of Rela and Traf3 notably increased after transfection with miR-155-5p inhibitor. Combined analysis using the TargetScan software and dual-luciferase gene reporter assays indicated that Rela and Traf3 were both targeted by miR-155-5p. Meanwhile, the expression of Ptgs2 also decreased after transfection of the AR42J cells with miR-155-5p mimic. The opposite results were found when miR-155-5p inhibitor was transfected into the AR42J cells. In addition, we treated caerulein- and TLC-S-induced AR42J cells with the Rela inhibitor helenalin and found that the expression of Rela, Traf3 and Ptgs2 decreased compared with the NC, while the expression of miR-155-5p did not show any significant difference. Furthermore, we found that miR-155-5p was significantly down-regulated in pancreatitis patients. Conclusion: miR-155-5p inversely regulated AP development through the Rela/Traf3/Ptgs2 signaling pathway.

show abstract

“…Similar studies [21][22][23][24][25] also show that the HTG induced by high-cholesterol diets, triglyceride infusion or the injection of an active detergent (Triton WR 1339) intensifies the course of pancreatitis. These findings support that both of endogenous and exogenous HTG increases susceptibility to acute pancreatitis.…”

Section: Pancreatic Histopathologic Examinationmentioning

confidence: 53%

Lipoprotein lipase gene-deficient mice with hypertriglyceridaemia associated with acute pancreatitis

et al. 2016

View full text Add to dashboard Cite

PURPOSE:To investigate the severity of pancreatitis in lipoprotein lipase (LPL)-deficient hypertriglyceridaemic (HTG) heterozygous mice and to establish an experimental animal model for HTG pancreatitis study. METHODS:LPL-deficient HTG heterozygous mice were rescued by somatic gene transfer and mated with wild-type mice. The plasma amylase, triglyceride, and pathologic changes in the pancreas of the LPL-deficient HTG heterozygous mice were compared with those of wild-type mice to assess the severity of pancreatitis. In addition, acute pancreatitis (AP) was induced by caerulein (50 µg/kg) for further assessment. RESULTS:The levels of plasma amylase and triglyceride were significantly higher in the LPL-deficient HTG heterozygous mice.According to the pancreatic histopathologic scores, the LPL-deficient HTG heterozygous mice showed more severe pathologic damage than the wild-type mice. CONCLUSIONS:Lipoprotein lipase deficient heterozygous mice developed severe caerulein-induced pancreatitis. In addition, their high triglyceride levels were stable. Therefore, LPL-deficient HTG heterozygous mice are a useful experimental model for studying HTG pancreatitis.

show abstract

From Moderately Severe to Severe Hypertriglyceridemia Induced Acute Pancreatitis: Circulating MiRNAs Play Role as Potential Biomarkers

Cited by 36 publications

References 39 publications

Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis

Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis

miR-155-5p is Negatively Associated with Acute Pancreatitis and Inversely Regulates Pancreatic Acinar Cell Progression by Targeting Rela and Traf3

Lipoprotein lipase gene-deficient mice with hypertriglyceridaemia associated with acute pancreatitis

Contact Info

Product

Resources

About