From oxidative imbalance to compromised standard sperm parameters: Toxicological aspect of phthalate esters on spermatozoa

Mondal, Shirsha; Bandyopadhyay, Asish

doi:10.1016/j.etap.2023.104085

“…Meanwhile, exposure to DMP in mice during early postnatal period can cause abnormal axon development in the CA3 region and reduce the dentate gyrus and CA3 neuron densities. Another study reported that feeding DMP can reduce the locomotor activities of mice and lead to depression-like pathological changes [19]. According to the existing toxicological mechanism researches, DMP can play a role by inducing oxidative stress injury, activating apoptotic signals, and in uencing the neurotransmitter release [20][21].…”

Section: Discussionmentioning

confidence: 99%

Dimethyl phthalate exposure induces cognitive impairment through COX2-mediated microglial activation

Wang,

Shen,

Lin

et al. 2024

Preprint

0

View full text Add to dashboard Cite

AIM We investigated the mechanism of action whereby exposure to dimethyl phthalate (DMP), an environmental pollutant, causes cognitive impairment. METHODS Network pharmacology was used to analyze the targets and regulatory networks associated with DMP-brain injury-cognitive impairment. The environmental intake of DMP was simulated by DMP exposure, and Morris water maze was adopted to examine cognitive impairment. The tissue levels of inflammatory cytokines were detected. Brain tissue damage was assayed by H&E and Nissl staining, and protein expression was identified by Western blotting. The effects of DMP were observed after separate application of siRNA-COX2 and celecoxib-COX2 inhibitors. In the in vitro experiment, the effects of DMP on microglia activation were investigated. RESULTS Network pharmacology revealed that PTGS2 (COX2) was significantly correlated with DMP, which could act through COX2. After exposure to DMP, mice showed significant brain injury and cognitive impairment, along with activation of microglia and upregulation of inflammatory cytokines. The application of siRNA-COX2 and celecoxib-COX2 could inhibit the effects of DMP and ameliorate the cognitive impairment in mice. The in vitro results showed that DMP could activate microglia and cause microglia activation and neuroinflammation. CONCLUSION DMP exposure can induce neuroinflammation through COX2-mediated activation of microglia, resulting in cognitive impairment. COX2 is an important target for DMP action.

show abstract

“…O BEHP induz conhecida toxicidade no sistema genital masculino como o dano testicular e infertilidade, disfunção na espermatogênese e síndrome da disgenesia testicular, caracterizada por hipospadia (abertura anormal da uretra peniana), criptorquidia, baixa qualidade do sêmen e câncer testicular, em fetos de gestantes expostas ao ftalato (Lin et al, 2023). Além disso, já foram relatadas alterações induzidas por ftalatos que comprometem a fertilidade, como a atrofia dos testículos e, consequentemente, a redução da qualidade e quantidade dos espermatozoides (Cannarella et al, 2023;Mondal;Bandyopadhyay, 2023). O desenvolvimento normal dos órgãos reprodutores masculinos também é prejudicado na presença de ftalatos, podendo causar anomalias congênitas da genitália externa e de órgãos reprodutores internos, como o epidídimo, o ducto deferente e a próstata (Foster, 2006).…”

Section: Palavras-chaveunclassified

Emergentes ambientais: alerta à proteção da saúde

2024

0

View full text Add to dashboard Cite

show abstract

“…O BEHP induz conhecida toxicidade no sistema genital masculino como o dano testicular e infertilidade, disfunção na espermatogênese e síndrome da disgenesia testicular, caracterizada por hipospadia (abertura anormal da uretra peniana), criptorquidia, baixa qualidade do sêmen e câncer testicular, em fetos de gestantes expostas ao ftalato (Lin et al, 2023). Além disso, já foram relatadas alterações induzidas por ftalatos que comprometem a fertilidade, como a atrofia dos testículos e, consequentemente, a redução da qualidade e quantidade dos espermatozoides (Cannarella et al, 2023;Mondal;Bandyopadhyay, 2023). O desenvolvimento normal dos órgãos reprodutores masculinos também é prejudicado na presença de ftalatos, podendo causar anomalias congênitas da genitália externa e de órgãos reprodutores internos, como o epidídimo, o ducto deferente e a próstata (Foster, 2006).…”

Section: Palavras-chaveunclassified