From polymeric particles to multifunctional nanocapsules for biomedical applications using the miniemulsion process

Landfester, Katharina; Musyanovych, Anna; Mailänder, Volker

doi:10.1002/pola.23786

Cited by 165 publications

(133 citation statements)

References 178 publications

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“…[22,23] The general concept of miniemulsions can be viewed as a process in which a heterophase system is subjected to high shear forces in order to generate small, homogeneous, and stable droplets of precursor materials in a continuous phase. The nanoparticle production using the miniemulsion process is shown in Figure 1.…”

Section: Synthetic Possibilities By the Use Of Miniemulsionsmentioning

confidence: 99%

Functional Hybrid Materials with Polymer Nanoparticles as Templates

Ethirajan

Landfester

2010

Chemistry A European J

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The use of polymeric nanoparticles as templates for producing inorganic materials is an intriguing approach as it offers the feasibility of synthesizing hybrid organic-inorganic functional materials for a broad spectrum of applications ranging from optoelectronics to biomedicine. The concept of using polymer nanoparticles as templates to produce hybrid materials has several advantages. On the one hand, the entire geometry of the nanoparticle can be used as a confined nano-environment to let the inorganic material grow inside the particle. On the other hand, the high surface area of nanoparticle can be exploited to let the inorganic material grow on the outside surface of the particles. One such application is presented here, in which polymer nanoparticles were used as biomimetic template to produce composite nanoparticles made of the bone mineral hydroxyapatite (HAP). The synthesized hybrid particle has a great potential to be used as regenerative filler or as scaffold for nucleation and growth of new bone material. In addition to be applied as coatings on implants, these nanoparticles also offer the feasibility of being injected directly into the damaged part or administered intravenously with functionalization. Within this overview, we will mainly focus on different polymer nanoparticles obtained by the miniemulsion technique and the different possibilities for them to be used as templates for the biomimetic mineralization of calcium phosphate in the aqueous phase.

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Section: Synthetic Possibilities By the Use Of Miniemulsionsmentioning

confidence: 99%

Functional Hybrid Materials with Polymer Nanoparticles as Templates

Ethirajan

Landfester

2010

Chemistry A European J

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“…Over the past few decades, submicron polymeric particles have been extensively investigated 2 especially as delivery systems in many fields, such as medicine, biotechnology as well as in 3 environmental, food, cosmetic or agrochemical applications [1][2][3]. Polymeric nanoparticles 4 (NPs) can be divided into nanospheres (NSs) and nanocapsules (NCs) depending on their 5 structure [4].…”

Section: Introductionmentioning

confidence: 99%

“…As already shown previously [34], this equation allows the Huckel's equations are applicable only under two limiting cases, i.e., ka >100 and ka <0. 1,195 respectively. ζ values were used to estimate the thickness of the adsorption layer ( PZ ) by 196 using the Eversole and Boardman equation [35]:…”

mentioning

confidence: 97%

Interfacial click chemistry for improving both dextran shell density and stability of biocompatible nanocapsules

Półtorak

Durand

Léonard

et al. 2015

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…We used polymeric polystyrene NPs synthesized by the miniemulsion process [9]. This manufacturing process allows the synthesis of consistent and long-term stable preparation of particles of comparable size.…”

Section: Introductionmentioning

confidence: 99%

Aggregation Behavior of Polystyrene-Nanoparticles in Human Blood Serum and its Impact on the in vivo Distribution in Mice

Mohr

Sommer²,

Baier

et al. 2014

J Nanomed Nanotechnol

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The interactions between nanoparticles (NPs) and proteins in complex biological application media such as blood serum are capable of inducing aggregate formation which can lead to subsequent changes in biological activity. Here, we correlate surface charge, aggregation-tendency, and surface serum protein adsorption with cellular uptake and biodistribution in mice.Polystyrene-based NPs (80 -170 nm) with different surface functionalizations were synthesized and incubated with human serum. Interaction of NPs with serum proteins and aggregate formation were analyzed by mass spectrometryanalysis and dynamic light-scattering. Influence of surface functionalization on specific cellular uptake and organdistribution was characterized.Localization and organ targeting of intravenously applied NPs preferentially depended on their aggregationbehavior in the presence of serum. Whereas strongly aggregating particles mainly located to liver, non-aggregating particles distributed to all organs. Determination of aggregate formation of NPs in the presence of serum and further analysis of the protein corona allows for pre-selection of NPs for in vivo application. studies were done by dynamic light scattering applying a previously developed pre-in vivo screening method [10]. This enabled us to analyze the effect of surface functionalization on aggregation and protein binding in the presence of human serum under physiologically relevant conditions. To allow analyzing the cellular uptake of NPs in cells of the peripheral blood as well as in professional antigen-presenting cells, the NPs were fluorescently labeled with boron-dipyrromethene (BODIPY) for flow cytometry and immune fluorescence. In addition, the particles were loaded with a near-infrared dye (IR-Dye 780) for in vivo imaging. Using quantitative high performance liquid chromatography coupled with mass spectrometry, we determined the composition of the protein corona. Using these particles, we were able to demonstrate that the pattern of protein binding in the presence of serum correlates with the tendency to form aggregates in vitro and in vivo. In addition, the charge of the particles' surface influenced their uptake in phagocytes and endocytosis as well as their biodistribution after intravenous application in mice.

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From polymeric particles to multifunctional nanocapsules for biomedical applications using the miniemulsion process

Cited by 165 publications

References 178 publications

Functional Hybrid Materials with Polymer Nanoparticles as Templates

Functional Hybrid Materials with Polymer Nanoparticles as Templates

Interfacial click chemistry for improving both dextran shell density and stability of biocompatible nanocapsules

Aggregation Behavior of Polystyrene-Nanoparticles in Human Blood Serum and its Impact on the in vivo Distribution in Mice

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