From Protein Features to Sensing Surfaces

Faccio, Greta

doi:10.3390/s18041204

Cited by 43 publications

(33 citation statements)

References 146 publications

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“…Immobilization on a support can increase or decrease the activity of an immobilized enzyme. Moreover, crowding on the surface of a support has been shown to reduce enzymatic activity because of limited active site accessibility, spatial restrictions and denaturation of the protein [ 65 ]. Thus, the concentration of the enzyme and binding method is relevant in order to obtain higher activity of the immobilized enzyme.…”

Section: Resultsmentioning

confidence: 99%

Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds

2020

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In this study, magnetic maghemite nanoparticles, which belong to the group of metal oxides, were functionalized with chitosan, a non-toxic, hydrophilic, biocompatible, biodegradable biopolymer with anti-bacterial effects. This was done using different synthesis methods, and a comparison of the properties of the synthesized chitosan functionalized maghemite nanoparticles was conducted. Characterization was performed using scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM). Characterizations of size distribution were performed using dynamic light scattering (DLS) measurements and laser granulometry. A chitosan functionalization layer was confirmed using potentiometric titration on variously synthesized chitosan functionalized maghemite nanoparticles, which is important for further immobilization of bioactive compounds. Furthermore, after activation of chitosan functionalized maghemite nanoparticles with glutaraldehyde (GA) or pentaethylenehexamine (PEHA), immobilization studies of enzyme cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) were conducted. Factors influencing the immobilization of enzymes, such as type and concentration of activating reagent, mass ratio between carrier and enzyme, immobilization time and enzyme concentration, were investigated. Briefly, microparticles made using the chitosan suspension cross-linking process (MC2) proved to be the most suitable for obtaining the highest activity of immobilized enzyme, and nanoparticles functionalized with chitosan using the covalent binding method (MC3) could compete with MC2 for their applications.

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Section: Resultsmentioning

confidence: 99%

Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds

2020

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“…Although ZIF-8 has become the most widely used framework for biomimetic mineralization, its hydrophobic nature can lead to inactive enzymes, as, e.g., in the case of catalase. Proteins often interact with hydrophobic surfaces with greater affinity, associated with conformational changes of the protein, [459] which can lead to deactivation. A recent work [460] has addressed this issue by investigating more hydrophilic frameworks ZIF-90 and MAF-7 for biomimetic mineralization in comparison to ZIF-8 ( Figure 51).…”

Section: Reticular Nanoparticles As Tools For Bionanotechnologymentioning

confidence: 99%

The Chemistry of Reticular Framework Nanoparticles: MOF, ZIF, and COF Materials

Ploetz

Engelke

Lächelt

et al. 2020

Adv Funct Materials

230

145

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Nanoparticles have become a vital part of a vast number of established processes and products; they are used as catalysts, in cosmetics, and even by the pharmaceutical industry. Despite this, however, the reliable and reproducible production of functional nanoparticles for specific applications remains a great challenge. In this respect, reticular chemistry provides methods for connecting molecular building blocks to nanoparticles whose chemical composition, structure, porosity, and functionality can be controlled and tuned with atomic precision. Thus, reticular chemistry allows for the translation of the green chemistry principle of atom economy to functional nanomaterials, giving rise to the multifunctional efficiency concept. This principle encourages the design of highly active nanomaterials by maximizing the number of integrated functional units while minimizing the number of inactive components. State-ofthe-art research on reticular nanoparticles-metal-organic frameworks, zeolitic imidazolate frameworks, and covalent organic frameworks-is critically assessed and the beneficial features and particular challenges that set reticular chemistry apart from other nanoparticle material classes are highlighted. Reviewing the power of reticular chemistry, it is suggested that the unique possibility to efficiently and straightforwardly synthesize multifunctional nanoparticles should guide the synthesis of customized nanoparticles in the future.

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“…The many methods used to immobilize biological ligands in well‐defined spatial arrangements on planar surfaces have been well reviewed elsewhere . Seemingly straightforward, the process of immobilization often affects protein functionality, and understanding of the physico‐chemical features of the protein surface can aid in selecting the optimal method of immobilization …”

Section: Reverse Engineering Of the Immune Microenvironmentmentioning

confidence: 99%

Deconstructing Immune Microenvironments of Lymphoid Tissues for Reverse Engineering

Witzel¹,

Nasser

Garcia-Sabaté

et al. 2018

Adv Healthcare Materials

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The immune microenvironment presents a diverse panel of cues that impacts immune cell migration, organization, differentiation, and the immune response. Uniquely, both the liquid and solid phases of every specific immune niche within the body play an important role in defining cellular functions in immunity at that particular location. The in vivo immune microenvironment consists of biomechanical and biochemical signals including their gradients, surface topography, dimensionality, modes of ligand presentation, and cell–cell interactions, and the ability to recreate these immune biointerfaces in vitro can provide valuable insights into the immune system. This manuscript reviews the critical roles played by different immune cells and surveys the current progress of model systems for reverse engineering of immune microenvironments with a focus on lymphoid tissues.

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From Protein Features to Sensing Surfaces

Cited by 43 publications

References 146 publications

Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds

Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds

The Chemistry of Reticular Framework Nanoparticles: MOF, ZIF, and COF Materials

Deconstructing Immune Microenvironments of Lymphoid Tissues for Reverse Engineering

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