Although general trends in transgressive to highstand sedimentary evolution of river-mouth coastlines are well-known, the details of the turnaround from retrogradational (typically estuarine) to aggradational-progradational (typically coastal/deltaic) stacking patterns are not fully resolved. This paper examines the middle to late Holocene eustatic highstand succession of the Po Delta: its stratigraphic architecture records a complex pattern of delta outbuilding and coastal progradation that followed eustatic stabilization, since around 7Á7 cal kyr BP. Sedimentological, palaeoecological (benthic foraminifera, ostracods and molluscs) and compositional criteria were used to characterize depositional conditions and sediment-dispersal pathways within a radiocarbon-dated chronological framework. A three-stage progradation history was reconstructed. First, as soon as eustasy stabilized (7Á7 to 7Á0 cal kyr BP), rapid bay-head delta progradation (ca 5 m year À1 ), fed mostly by the Po River, took place in a mixed, freshwater and brackish estuarine environment. Second, a dominantly aggradational parasequence set of beachbarrier deposits in the lower highstand systems tract (7Á0 to 2Á0 cal kyr BP) records the development of a shallow, wave-dominated coastal system fed alongshore, with elongated, modestly crescent beaches (ca 2Á5 m year À1 ). Third, in the last 2000 years, the development of faster accreting and more rapidly prograding (up to ca 15 m year À1 ) Po delta lobes occurred into 30 m deep waters (upper highstand systems tract). This study documents the close correspondence of sediment character with stratal distribution patterns within the highstand systems tract. Remarkable changes in sediment characteristics, palaeoenvironments and direction of sediment transport occur across a surface named the 'A-P surface'. This surface demarcates a major shift from dominantly aggradational (lower highstand systems tract) to fully progradational (upper highstand systems tract) parasequence stacking. In the Po system, this surface also reflects evolution from a wave-dominated to 3029 river-dominated deltaic system. Identifying the A-P surface through detailed palaeoecological and compositional data can help guide interpretation of highstand systems tracts in the rock record, especially where facies assemblages and their characteristic geometries are difficult to discern from physical sedimentary structures alone.