a b s t r a c t a r t i c l e i n f oObjective. This study examined the occurrence and duration of sedentary bouts and explored the crosssectional association with health indicators in children applying various operational definitions of sedentary bouts.Methods. Accelerometer data of 647 children (10-13 years old) were collected in five European countries. We analyzed sedentary time (b 100 cpm) accumulated in bouts of at least 5, 10, 20 or 30 min based on four operational definitions, allowing 0, 30 or 60 s ≥100 cpm within bouts. Health indicators included anthropometrics (i.e. waist circumference and body mass index (BMI)) and in a subsample from two European countries (n = 112) fasting capillary blood levels of glucose, C-peptide, high-density-and low-density cholesterol, and triglycerides. Data collection took place from March to July 2010. Associations were adjusted for age, gender, moderate-to-vigorous physical activity, total wear time and country.Results. Occurrence of sedentary bouts varied largely between the various definitions. Children spent most of their sedentary time in bouts of ≥5 min while bouts of ≥20 min were rare. Linear regression analysis revealed few significant associations of sedentary time accumulated in bouts of ≥5-30 min with health indicators. Moreover, we found that more associations became significant when allowing no tolerance time within sedentary bouts.Conclusion. Despite a few significant associations, we found no convincing evidence for an association between sedentary time accumulated in bouts and health indicators in 10-13 year old children.
IntroductionRecent experimental studies in normal weight young (Altenburg et al., 2013;Peddie et al., 2013) and overweight adults (Dunstan et al., 2012) indicate that, compared to uninterrupted sitting, brief, light-or moderate-intensity breaks during prolonged sitting (i.e. 1 min 40 s breaks every 30 min) may attenuate cardiometabolic risk. These studies support the hypothesized mechanism that prolonged sitting leads to the loss of contractile stimulation in weight-bearing muscles, which suppresses skeletal muscle lipoprotein lipase (LPL) activity, leading to a prolonged time in which cellular metabolism substrates are present in the vascular compartments (Bey and Hamilton, 2003;Hamilton et al., 2004). These effects may contribute to the development of cardiovascular diseases. In contrast to studies in adults, brief light-intensity interruptions in sitting did not result in measurable changes in cardiometabolic indicators in 10-14 year old children (Saunders et al., 2013a).Currently, few epidemiological studies have examined accelerometerbased sedentary bouts and breaks in sedentary time in children (Colley Preventive Medicine 71 (2015)