From SELDI-TOF MS to protein identification by on-chip elution

Nilsen, Mari Mæland; Uleberg, Kai-Erik; Janssen, Emiel A. M.; Baak, J. P. A.; Andersen, Odd Ketil; Hjelle, Anne

doi:10.1016/j.jprot.2011.06.027

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…In addition, the reproducibility of peak intensity was poor and protein identification cannot be made based solely on SELDI-TOF MS spectra. Furthermore, serum protein biomarkers were not detected in patients with treated TB or those with other lung diseases [17,18]. …”

Section: Discussionmentioning

confidence: 99%

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Shi

Wei

et al. 2017

PLoS ONE

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The aim of this study was to discover novel biomarkers for pulmonary tuberculosis (TB). Differentially expressed proteins in the serum of patients with TB were screened and identified by iTRAQ-two dimensional liquid chromatography tandem mass spectrometry analysis. A total of 79 abnormal proteins were discovered in patients with TB compared with healthy controls. Of these, significant differences were observed in 47 abnormally expressed proteins between patients with TB or pneumonia and chronic obstructive pulmonary disease (COPD). Patients with TB (n = 136) exhibited significantly higher levels of serum amyloid A (SAA), vitamin K-dependent protein Z (PROZ), and C4b-binding protein β chain (C4BPB) than those in healthy controls (n = 66) (P<0.0001 for each) albeit significantly lower levels compared with those in patients with pneumonia (n = 72) (P<0.0001 for each) or COPD (n = 72) (P<0.0001, P<0.0001, P = 0.0016, respectively). After 6 months of treatment, the levels of SAA and PROZ were significantly increased (P = 0.022, P<0.0001, respectively), whereas the level of C4BPB was significantly decreased (P = 0.0038) in treated TB cases (n = 72). Clinical analysis showed that there were significant differences in blood clotting and lipid indices in patients with TB compared with healthy controls, patients with pneumonia or COPD, and treated TB cases (P<0.05). Correlation analysis revealed significant correlations between PROZ and INR (rs = 0.414, P = 0.044), and between C4BPB and FIB (rs = 0.617, P = 0.0002) in patients with TB. Receiver operating characteristic curve analysis revealed that the area under the curve value of the diagnostic model combining SAA, PROZ, and C4BPB to discriminate the TB group from the healthy control, pneumonia, COPD, and cured TB groups was 0.972, 0.928, 0.957, and 0.969, respectively. Together, these results suggested that SAA, PROZ, and C4BPB may serve as new potential biomarkers for TB. Our study may thus provide experimental data for the differential diagnosis of TB.

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Section: Discussionmentioning

confidence: 99%

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Shi

Wei

et al. 2017

PLoS ONE

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“…Capillary electrophoresis coupled to MS represents ideal analytical technique for different omics approaches that provides high resolution protein separation based on differential migration through a buffer-filled capillary column in an electrical field (300 to 500 V/cm) [ 54 , 55 ]. CE can be coupled either with MALDI (off-line) or ESI (on-line).…”

Section: Techniques For Urinary Proteomic Studiesmentioning

confidence: 99%

Human Urine Proteomics: Analytical Techniques and Clinical Applications in Renal Diseases

Kalantari

Jafari

Moradpoor

et al. 2015

International Journal of Proteomics

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Urine has been in the center of attention among scientists of clinical proteomics in the past decade, because it is valuable source of proteins and peptides with a relative stable composition and easy to collect in large and repeated quantities with a noninvasive procedure. In this review, we discuss technical aspects of urinary proteomics in detail, including sample preparation, proteomic technologies, and their advantage and disadvantages. Several recent experiments are presented which applied urinary proteome for biomarker discovery in renal diseases including diabetic nephropathy, immunoglobulin A (IgA) nephropathy, focal segmental glomerulosclerosis, lupus nephritis, membranous nephropathy, and acute kidney injury. In addition, several available databases in urinary proteomics are also briefly introduced.

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“…Bound proteins of a spot were eluted with 100 µL of 50 mM ammonium bicarbonate solution (AB), overnight at 4℃ with slow agitation 10 ; the second spot was used as load control. Eluted proteins were collected for ‘in-liquid’ tryptic digestion.…”

Section: Methodsmentioning

confidence: 99%

New horizon in dialysis depuration: Characterization of a polysulfone membrane able to break the ‘albumin wall’

Cuoghi

Caiazzo²,

Monari

et al. 2014

J Biomater Appl

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The uremic syndrome is attributed to the progressive retention of a large number of toxins, which under normal conditions are excreted by the healthy kidneys. Standard dialytic membranes do not purify middle-high molecular weight toxins. Haemodiafiltration with endogenous reinfusion coupled with a highly permeable membrane could break the limit of the 'albumin wall' improving the dialytic depuration without loss of important nutrients. The aim of this study was to evaluate the performance of a new polysulfone membrane, Synclear 0.2, to remove uremic molecules. Surface Enhanced Laser Desorption Ionization-Time of Flight was employed to evaluate the proteomic profile of ultrafiltrate and Electrospray Ionization-Quadruple-ToF coupled with on-chip elution was used for proteins identification. A high and specific permeability for middle-high molecular weight molecules was revealed by mass spectrometry for the investigated membrane. The identified proteins are mostly uremic toxins: their relative abundance, estimated in the ultrafiltrate by exponentially modified protein abundance index, showed a high purification efficiency of the new membrane when compared with conventional ones. In conclusion, Synclear 0.2, used as convective membrane in hemodiafiltration with endogenous reinfusion treatment, permits to break the 'albumin wall', clearing middle-high molecular weight uremic toxins, improving the dialytic treatment purification efficiency.

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From SELDI-TOF MS to protein identification by on-chip elution

Cited by 9 publications

References 15 publications

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Human Urine Proteomics: Analytical Techniques and Clinical Applications in Renal Diseases

New horizon in dialysis depuration: Characterization of a polysulfone membrane able to break the ‘albumin wall’

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