The Xinjiang region is a crossroad between the West and East on the Silk Road, where many exquisite hair textile relics in the Bronze Age were unearthed, therefore, it attracts the attention of scholars around the world. However, the limitation of information acquisition ability is still the main bottleneck to recognize the scientific value of ancient hair textile relics. In this study, paleoproteomics method was proposed to analyse the hair textile relics from Xiaohe and Shanpula cemetery in the Xinjiang region. To establish the databases of biomarkers and morphological features, five modern hair samples from representative animals (grey fox, raccoon dog, American mink, cape hare, and lamb) were selected and examined. Through searching the databases, the ancient hair textile samples from Xiaohe and Shanpula cemetery were all identified as wool. Ultimately, this work demonstrated the evidences supporting the existence of prosperous sheep husbandry, wool manufacture and cultural exchange along the Silk Road in the Bronze Age. The paleoproteomics, in combination with other methods, obtained more reliable and profound information from hair textile relics than ever before, and it goes without saying that they have bright prospects of being widely used in hair textile archaeology.