From the inside out: Elemental compositions and mineral phases provide insights into bacterial calcification

Lyu, Jiejie; Li, Fuchun; Zhang, Chonghong; Gower, Laurie B.; Wasman, Scott J.; Jun, Sun; Yang, Guoguo; Chen, Jiani; Gu, Lixin; Tang, Xu; Scheiffele, Gary W.

doi:10.1016/j.chemgeo.2020.119974

Cited by 22 publications

(13 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacterial cells or EPS could serve as a structural unit for calcium carbonate precipitation and help start the biomineralization process. 43 Moreover, the addition of bacteria can greatly contribute to the deposition of calcium carbonate. 44 Carbonic anhydrases (CA) catalyze the carbon dioxide hydration reaction and further increase pH values in the culture medium.…”

Section: Discussionmentioning

confidence: 99%

Carbonate biomineralization differentially induced by two psychrophilic Pseudomonas psychrophila strains isolated from an alpine travertine landform

Song

Zhou

et al. 2021

RSC Adv.

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Carbonate biomineralization differentially induced by two psychrophilic Pseudomonas psychrophila strains isolated from an alpine travertine landform

Song

Zhou

et al. 2021

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…A non-pathogenic LV-1 ( Bacillus cereus ) was bought from the China General Microbiological Culture Collection Center (CGMCC 1.15914). 9 A transmission electron microscope image shows that the cells are rod-shaped with a size of (1.0–1.2) μm × (4.0–6.0) μm ( Fig. 1 ), arranged as a short or long chain.…”

Section: Methodsmentioning

confidence: 99%

“…7 Bacterial cells and extracellular polymeric substances (EPS) can inuence the nucleation and growth of minerals by serving as nucleation sites. 8,9 Calcium carbonate (CaCO 3 ) has three anhydrous crystalline polymorphs, calcite, aragonite, and vaterite, as well as one amorphous calcium carbonate (ACC). Calcite is the most stable, and aragonite and vaterite are generally metastable minerals.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effect of Bacillus cereus LV-1 on the crystallization and polymorphs of calcium carbonate

Yang

Wang

et al. 2022

RSC Adv.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The two most thermodynamically stable crystal structures common to MICP are aragonite and calcite (Stocks-Fischer et al, 1999;Hammes and Verstraete, 2002;Banks et al, 2010;Seifan and Berenjian, 2019) with vaterite acting as a metastable phase transient intermediate which favors the more stable aragonite and calcite (Christy, 2017). Bacteria that perform MICP then become self-fossilized and act as nucleation sites for further calcium carbonate crystal growth, which in turn aids in the process of speleothem formation (Palmer, 1991;Northup and Lavoie, 2001;Türkgenci and Dogruöz Güngör, 2011;Seifan and Berenjian, 2019;Lyu et al, 2021). Urease enzymes are broadly distributed throughout the bacterial domain, always occurring as multimeric proteins with nickel incorporated into the enzyme structure (Mobley et al, 1995;Konieczna et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Biomineralization in Cave Bacteria—Popcorn and Soda Straw Crystal Formations, Morphologies, and Potential Metabolic Pathways

et al. 2022

View full text Add to dashboard Cite

Caves are extreme, often oligotrophic, environments that house diverse groups of microorganisms. Many of these microbes can perform microbiologically induced carbonate precipitation (MICP) to form crystalline secondary cave deposits known as speleothems. The urease family is a group of enzymes involved in MICP that catalyze the breakdown of urea, which is a source of energy, into ammonia and carbonate. Carbonate anions are effluxed to the extracellular surface of the bacterium where it then binds to environmental calcium to form calcium carbonate which then continues to grow in crystal form. Here, we studied bacterial communities from speleothems collected from the Iron Curtain Cave (ICC) in Chilliwack, B.C., Canada, to characterize these organisms and determine whether urease-positive (U+) bacteria were present in the cave and their potential impact on speleothem formation. The ICC is a carbonate cave located on the northside of Chipmunk Ridge, presenting a unique environment with high iron content sediment and limestone structures throughout. With six pools of water throughout the cave, the environment is highly humid, with temperatures ranging between 4 and 12°C depending on the time of year. Ninety-nine bacterial strains were isolated from popcorn (PCS) and soda straw (SSS) speleothems. These isolates were screened for urease enzymatic activity, with 11 candidates found to be urease-positive. After incubation, species-specific crystal morphologies were observed. Popcorn speleothem provided more bacterial diversity overall when compared to soda straw speleothem when examined under a culture-based method. Nearly twice as many U+ isolates were isolated from popcorn speleothems compared to soda straw speleothems. The U+ candidates were identified to the genus level by 16S rRNA analysis, and two isolates underwent whole-genome sequencing. Two novel species were identified as Sphingobacterium sp. PCS056 and Pseudarthrobacter sp. SSS035. Both isolates demonstrated the most crystal production as well as the most morphologically dissimilar crystal shapes in broth culture and were found to produce crystals as previously observed in both agar and broth media. The results from this study are consistent with the involvement of urease-positive bacteria isolated from the ICC in the formation of cave speleothems. 16S rRNA sequencing revealed a diverse set of microbes inhabiting the speleothems that have urease activity. Whole-genome sequencing of the two chosen isolates confirmed the presence of urease pathways, while revealing differences in urease pathway structure and number. This research contributes to understanding microbial-associated cave formation and degradation, with applications to cave conservation, microbiota composition, and their role in shaping the cave environment.

show abstract

From the inside out: Elemental compositions and mineral phases provide insights into bacterial calcification

Cited by 22 publications

References 73 publications

Carbonate biomineralization differentially induced by two psychrophilic Pseudomonas psychrophila strains isolated from an alpine travertine landform

Carbonate biomineralization differentially induced by two psychrophilic Pseudomonas psychrophila strains isolated from an alpine travertine landform

The effect of Bacillus cereus LV-1 on the crystallization and polymorphs of calcium carbonate

Biomineralization in Cave Bacteria—Popcorn and Soda Straw Crystal Formations, Morphologies, and Potential Metabolic Pathways

Contact Info

Product

Resources

About