From the laboratory to the wild: salinity-based genetic differentiation of the European sea bass (Dicentrarchus labrax) using gene-associated and gene-independent microsatellite markers

Guinand, Bruno; Quéré, Nolwenn; Desmarais, Érick; Lagnel, Jacques; Tsigenopoulos, Costas S.; Bonhomme, François

doi:10.1007/s00227-014-2602-8

Cited by 12 publications

(7 citation statements)

References 131 publications

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“…Together with observed differences in body size among E individuals from each lagoon, these results suggest that early selection may have occurred at or just after settlement in response to specific selective pressures in each lagoon. A sequential temporal genetic response to a sea−lagoon selective gradient was already suggested for young sea bass recruits in the MA lagoon, with a possible signature of early selection appearing very soon after colonisation, while further selective differences were only observed after some months (Guinand et al 2015) (see also Planes & Roman 2004 for the white sea bream Diplodus sargus). In addition, Chaoui et al (2012) showed that a mechanism based on genotype-dependent habitat choice with no selective mortality was very unlikely to produce the observed genetic structure at the GH and Prl loci.…”

Section: Discussionmentioning

confidence: 77%

Candidate gene variation in gilthead sea bream reveals complex spatiotemporal selection patterns between marine and lagoon habitats

Guinand¹,

Chauvel²,

Lechene³

et al. 2016

Mar. Ecol. Prog. Ser.

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In marine fishes, the extent to which spatial patterns induced by selection remain stable across generations remains largely unknown. In the gilthead sea bream Sparus aurata, polymorphisms in the growth hormone (GH) and prolactin (Prl) genes can display high levels of differentiation between marine and lagoon habitats. These genotype−environment associations have been attributed to differential selection following larval settlement, but it remains unclear whether selective mortality during later juvenile stages further shapes genetic differences among habitats. We addressed this question by analysing differentiation patterns at GH and Prl markers together with a set of 21 putatively neutral microsatellite loci. We compared genetic variation of spring juveniles that had just settled in 3 ecologically different lagoons against older juveniles sampled from the same sites in autumn, at the onset of winter outmigration. In spring, genetic differentiation among lagoons was greater than expected from neutrality for both candidate gene markers. Surprisingly, this signal disappeared completely in the older juveniles, with no significant differentiation for either locus a few months later in autumn. We searched for signals of haplotype structure within GH and Prl genes using next-generation amplicon deep sequencing. Both genes contained 2 groups of haplotypes, but high similarities among groups indicated that signatures of selection, if any, had largely been erased by recombination. Our results are consistent with the view that differential selection operates during early juvenile life in sea bream and highlight the importance of temporal replication in studies of post-settlement selection in marine fish.

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Section: Discussionmentioning

confidence: 77%

Candidate gene variation in gilthead sea bream reveals complex spatiotemporal selection patterns between marine and lagoon habitats

Guinand¹,

Chauvel²,

Lechene³

et al. 2016

Mar. Ecol. Prog. Ser.

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“…Quéré et al . () reported a clinal pattern of genetic differentiation possibly supporting adaptive variation at one single anonymous microsatellite locus ( DLA0068 ) among the 21 loci of sea bass studied (but see Guinand et al ., ). The only other documented case in the Mediterranean Sea suggests that European hake carrying the Gapdh 120 and Gpi2 96 alleles might be better adapted to salinity (Cimmaruta et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Larmuseau et al ., ; Williams & Oleksiak, ; Eizaguirre et al ., ; Jones et al ., ). While so far only the EIF3E marker gene has been identified to play a role in local adaptation of sea bass to salinity (Guinand et al ., ), evidence of the growth hormone, prolactin and SL genes on a regional scale (Quéré et al ., ) has been criticized on the grounds of experimental bias (Guinand et al ., ). However, the SL hormone appears also in our genome scan.…”

Section: Discussionmentioning

confidence: 99%

Range-wide population structure of European sea bassDicentrarchus labrax

Souche

Hellemans

Babbucci

et al. 2015

Biol. J. Linn. Soc.

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The euryhaline European sea bass Dicentrarchus labrax L., inhabiting the coasts of the eastern Atlantic Ocean and Mediterranean Sea, has had many opportunities for differentiation throughout its large natural range. However, evidence for this has been incompletely documented geographically and with an insufficient number of markers. Therefore, its full range was sampled at 22 sites and individuals were genotyped with a suite of mapped markers, including 14 microsatellite loci (N = 536) and 46 neutral or gene‐linked single nucleotide polymorphisms (SNPs; N = 644). We confirm that the Atlantic and Mediterranean basins harbour two distinct lineages. Within the Atlantic Ocean no pattern was obvious based on the microsatellite and SNP genotypes, except for a subtle difference between South‐eastern and North‐eastern Atlantic sea bass attributed to limited introgression of alleles of Mediterranean origin. SNP genotypes of the Mediterranean lineage differentiated into three groups, probably under the influence of geographical isolation. The Western Mediterranean group showed genetic homogeneity without evidence for outlier loci. The Adriatic group appeared as a distinct unit. The Eastern Mediterranean group showed a longitudinal gradient of genotypes and most interestingly an outlier locus linked to the somatolactin gene. Overall, the spatial pattern fits those observed with other taxa of between‐basin segregation and within‐basin connectivity, which concurs well with the swimming capabilities of European sea bass. Evidence from a few outlier loci in this and other studies encourages further exploration of its regional connectivity and adaptive evolution.

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“…If evidence of adaptation to higher salinities is found in E. complanata populations, these approaches could be implemented to identify candidate loci underpinning adaptation. The problem of false positives is a difficult one, however, as noted by Guinand et al and others.…”

Section: Discussionmentioning

confidence: 99%

RNA sequencing analysis of transcriptional change in the freshwater mussel Elliptio complanata after environmentally relevant sodium chloride exposure

Robertson

Galbraith

Iwanowicz

et al. 2017

Enviro Toxic and Chemistry

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To identify potential biomarkers of salt stress in a freshwater sentinel species, we examined transcriptional responses of the common mussel Elliptio complanata to controlled sodium chloride (NaCl) exposures. Ribonucleic acid sequencing (RNA-Seq) of mantle tissue identified 481 transcripts differentially expressed in adult mussels exposed to 2 ppt NaCl (1.2 ppt chloride) for 7 d, of which 290 had nonoverlapping intervals. Differentially expressed gene categories included ion and transmembrane transport, oxidoreductase activity, maintenance of protein folding, and amino acid metabolism. The rate-limiting enzyme for synthesis of taurine, an amino acid frequently linked to osmotic stress in aquatic species, was upregulated, as was the transmembrane ion pump sodium/potassium adenosine 5'-triphosphatase. These patterns confirm a primary transcriptional response to the experimental dose, albeit likely overlapping with nonspecific secondary stress responses. Substantial involvement of the heat shock protein 70 chaperone family and the water-transporting aquaporin family was not detected, however, in contrast to some studies in other bivalves. A subset of the most significantly regulated genes was confirmed by quantitative polymerase chain reaction in an independent sample. Cluster analysis showed separation of mussels exposed to 2 ppt NaCl from control mussels in multivariate space, but mussels exposed to 1 ppt NaCl were largely indistinguishable from controls. Transcriptome-scale analysis of salt exposure under laboratory conditions efficiently identified candidate biomarkers for further functional analysis and field validation. Environ Toxicol Chem 2017;36:2352-2366. © Published 2017 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.

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From the laboratory to the wild: salinity-based genetic differentiation of the European sea bass (Dicentrarchus labrax) using gene-associated and gene-independent microsatellite markers

Cited by 12 publications

References 131 publications

Candidate gene variation in gilthead sea bream reveals complex spatiotemporal selection patterns between marine and lagoon habitats

Candidate gene variation in gilthead sea bream reveals complex spatiotemporal selection patterns between marine and lagoon habitats

Range-wide population structure of European sea bassDicentrarchus labrax

RNA sequencing analysis of transcriptional change in the freshwater mussel Elliptio complanata after environmentally relevant sodium chloride exposure

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