From the stem of the placental tree: trophoblast stem cells and their progeny

Latos, Paulina A.; Hemberger, Myriam

doi:10.1242/dev.133462

Cited by 108 publications

(106 citation statements)

References 94 publications

(156 reference statements)

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“…In post-implantation embryos, donor mCherry + EPSCs were found both in the embryo proper and in the trophectoderm-derived Elf5 + extra-embryonic ectoderm (ExE) region20 in about 35% of the 6.5 days post-coitum (d.p.c.) chimaeras (78 out of 225) (Fig.…”

mentioning

confidence: 99%

Establishment of mouse expanded potential stem cells

Yang

Ryan

Wang

et al. 2017

Nature

251

240

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Mouse embryonic stem cells derived from the epiblast1 contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm2 upon reintroduction to the blastocyst. Here we report that cultures of expanded potential stem cells can be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells. Remarkably, a single expanded potential stem cell can contribute both to the embryo proper and to the trophectoderm lineages in a chimaera assay. Bona fide trophoblast stem cell lines and extra-embryonic endoderm stem cells can be directly derived from expanded potential stem cells in vitro. Molecular analyses of the epigenome and single-cell transcriptome reveal enrichment for blastomere-specific signature and a dynamic DNA methylome in expanded potential stem cells. The generation of mouse expanded potential stem cells highlights the feasibility of establishing expanded potential stem cells for other mammalian species.

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confidence: 99%

Establishment of mouse expanded potential stem cells

Yang

Ryan

Wang

et al. 2017

Nature

251

240

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“…However, the commitment of TE is missing in this report. Considering trophoblast stem cells can be derived from either E3.5 blastocyst or E6.5 extra-embryonic ectoderm [10]. It'll be interesting to investigate the fate of TE during this transition stage at single cell level.…”

Section: Discussionmentioning

confidence: 99%

Cell Lineage Specification at Single Cell Resolution

Yang

2017

Single Cell Biol

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Single cell technology has been widely used in developmental and stem cell biology. In mouse, single cell transcriptome has revealed the cell lineage specification in pre-implantation and post-implantation stage embryo. Now Mohammed et al. investigated the dynamic cell fate commitment during the transition from peri-implantation to early post-implantation stage with single cell RNA sequencing. Except confirmation of some previous findings, the time window and cell sub clusters of cell specification is more precisely determined, along with possible new mechanism for X re-activation and inactivation in female embryo and for exit from pluripotency and lineage commitment. These data will not only fill the missing link in mouse embryo development, but provide insights into embryo development of other mammalian species including human.

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“…3 Trophoblast cells function in diverse biologic processes, such as embryo implantation, placental development, and key steps of the maternal cycle. 3 Trophoblast cells function in diverse biologic processes, such as embryo implantation, placental development, and key steps of the maternal cycle.…”

Section: Introductionmentioning

confidence: 99%

“…Trophoblast cells are derived from fertilized eggs and are the only fetal cells in direct contact with the parent system. 3 Trophoblast cells function in diverse biologic processes, such as embryo implantation, placental development, and key steps of the maternal cycle. 4,5 Abnormal growth and differentiation of trophoblast cells can result in adverse pregnancy outcomes, such as spontaneous abortion, fetal growth restriction, hypertensive disease of pregnancy, malignant hydatidiform mole, and choriocarcinoma.…”

Section: Introductionmentioning

confidence: 99%