From workout to molecular switches: How does skeletal muscle produce, sense, and transduce subcellular redox signals?

Henriquez-Olguin, Carlos; Meneses-Valdes, Roberto; Kritsiligkou, Paraskevi; Fuentes-Lemus, Eduardo

doi:10.1016/j.freeradbiomed.2023.10.404

Cited by 8 publications

(1 citation statement)

References 88 publications

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“…Skeletal muscle studies are ideally placed to pioneer single cell oxidative stress research, especially when clear fibre-type differences (Cobley et al, 2016;Vasileiadou et al, 2023) make such analysis phenotypically meaningful (Rosenberger et al, 2023). They can also address lingering questions in redox signalling (Fuentes-Lemus and Glover et al, 2023;Henriquez-Olguin et al, 2023), particularly around the spatial regulation of the process with the potential for phase transition effects (Dai et al, 2023;Kritsiligkou et al, 2023). Finally, the field is set to play a leading role in unravelling the addressing individuality at the dawn of the personalised redox biology era (Margaritelis, 2023).…”

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confidence: 99%

Ten “Cheat Codes” for Measuring Oxidative Stress in Humans

Cobley,

Margaritelis,

Chatzinikolaou

et al. 2024

Preprint

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Formidable and often seemingly insurmountable conceptual, technical, and methodological challenges hamper the measurement of oxidative stress in humans. For instance, fraught and flawed methods, such as the thiobarbituric acid reactive substances assay kits for lipid peroxidation, rate-limit progress. To advance translational redox research, we present ten comprehensive “cheat codes” for measuring oxidative stress in humans. The cheat codes include analytical approaches to assess reactive oxygen species, antioxidants, biomarkers of oxidative damage and redox regulation. They provide essential conceptual, technical, and methodological information inclusive of curated “do” and “don’t” guidelines. Given the biochemical complexity of oxidative stress, we present a research question-grounded decision tree guide for selecting the most appropriate cheat code (s) to implement in a prospective human experiment. Worked examples demonstrate the benefits of the decision tree-based cheat code selection tool. The ten cheat codes define an invaluable resource for measuring oxidative stress in humans.

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confidence: 99%

Ten “Cheat Codes” for Measuring Oxidative Stress in Humans

Cobley,

Margaritelis,

Chatzinikolaou

et al. 2024

Preprint

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Unresolved questions in the regulation of skeletal muscle insulin action by reactive oxygen species

Gallero,

Persson,

Henríquez‐Olguín

2024

FEBS Letters

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Reactive oxygen species (ROS) are well‐established signaling molecules implicated in a wide range of cellular processes, including both oxidative stress and intracellular redox signaling. In the context of insulin action within its target tissues, ROS have been reported to exert both positive and negative regulatory effects. However, the precise molecular mechanisms underlying this duality remain unclear. This Review examines the complex role of ROS in insulin action, with a particular focus on skeletal muscle. We aim to address three critical aspects: (a) the proposed intracellular pro‐oxidative redox shift elicited by insulin, (b) the evidence supporting that redox‐sensitive cysteine modifications impact insulin signaling and action, and (c) cellular mechanisms underlying how ROS can paradoxically act as both enhancers and inhibitors of insulin action. This Review underscores the urgent need for more systematic research to identify specific reactive species, redox targets, and the physiological significance of redox signaling in maintaining insulin action and metabolic health, with a particular emphasis on human skeletal muscle.

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