Front Cover: Multiplexed Delivery of Synthetic (Un)Conjugatable Ubiquitin and SUMO2 Enables Simultaneous Monitoring of Their Localization and Function in Live Cells (ChemBioChem 11/2022)

Mann, Guy; Sadhu, Pradeep; Brik, Ashraf

doi:10.1002/cbic.202200150

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Physical Methods1

Cell-permeable Ub/ubl-abps1

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2023

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(2 citation statements)

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“…[23] Recently, Brik et al found that this method can simultaneously load up to four different proteins into live cells. [24]…”

Section: Physical Methodsmentioning

confidence: 99%

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Cell‐Permeable Ubiquitin and Histone Tools for Studying Post‐translational Modifications

et al. 2023

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Protein post‐translational modifications (PTMs) regulate nearly all biological processes in eukaryotic cells, and synthetic PTM protein tools are widely used to detect the activity of the related enzymes and identify the interacting proteins in cell lysates. Recently, the study of these enzymes and the interacting proteome has been accomplished in live cells using cell‐permeable PTM protein tools. In this concept, we will introduce cell penetrating techniques, the syntheses of cell‐permeable PTM protein tools, and offer some future perspective.

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“…[23] Recently, Brik et al found that this method can simultaneously load up to four different proteins into live cells. [24]…”

Section: Physical Methodsmentioning

confidence: 99%

“…In addition, Birk et al also developed several Ub and SUMO fluorescent probes carrying different fluorophores that are simultaneously delivered by multiplexed bead loading to visualize ubiquitination and SUMOylation events in live cells. [24]…”

Section: Cell-permeable Ub/ubl-abpsmentioning

confidence: 99%