Frontispiz: A Highly Compatible Phototrophic Community for Carbon‐Negative Biosynthesis

Li, Chaofeng; Wang, Ruoyu; Wang, Jiawei; Liu, Liangxu; Li, Hengrun; Zheng, Haotian; Ni, Jun

doi:10.1002/ange.202380261

Cited by 1 publication

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“…13 Cyanobacteria utilizing CO 2 to synthesize ferulic acid, which was then used as the substrate for E. coli, produced 20.8 mg/L curcumin. 16 In addition to these rational design strategies, library construction has also been effective for curcumin production. Multiple Automated Genome Engineering (MAGE) tools have been used to screen mutant libraries of the 5′-untranslated region (5′-UTR) sequence to fine-tune expression of the key enzymes involved in the curcumin biosynthetic pathway; this de novo biosynthesis of curcumin achieved a titer of 3.9 ± 0.8 mg/L, the highest previously reported from this approach.…”

Section: Introductionmentioning

confidence: 99%

Efficient De Novo Biosynthesis of Curcumin in Escherichia coli by Optimizing Pathway Modules and Increasing the Malonyl-CoA Supply

Chen,

Wang,

Wang

et al. 2023

J. Agric. Food Chem.

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Curcumin is a natural phenylpropanoid compound with various biological activities and is widely used in food and pharmaceuticals. A de novo curcumin biosynthetic pathway was constructed in Escherichia coli BL21(DE3). Optimization of the curcumin biosynthesis module achieved a curcumin titer of 26.8 ± 0.6 mg/L. Regulating the metabolic fluxes of the β-oxidation pathway and fatty acid elongation cycle and blocking the endogenous malonyl-CoA consumption pathway increased the titer to 113.6 ± 7.1 mg/L. Knockout of endogenous curcumin reductase (curA) and intermediate product detoxification by heterologous expression of the solvent-resistant pump (srpB) increased the titer to 137.5 ± 3.0 mg/L. A 5 L pilot-scale fermentation, using a threestage pH alternation strategy, increased the titer to 696.2 ± 20.9 mg/L, 178.5-fold higher than the highest curcumin titer from de novo biosynthesis previously reported, thereby laying the foundation for efficient biosynthesis of curcumin and its derivatives.

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Section: Introductionmentioning

confidence: 99%