Frosted corn silage with or without bacterial inoculants in dairy cattle ration

Mohammadzadeh, Hamid; Khorvash, M.; Ghorbani, G.R.; Yang, Wenzhu

doi:10.1016/j.livsci.2012.01.011

Cited by 20 publications

(22 citation statements)

References 36 publications

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“…These findings implied that fermentation rate can be limited in frosted or matured crops due to a higher proportion of DM, a lower concentration of WSC and fewer LAB. Furthermore, the greater number of fungi on matured and frosted crops may enhance spoilage of produced silage (Mohammadzadeh et al 2012). A higher concentration of lactic acid was found in silages at an earlier maturity stage.…”

Section: Discussionmentioning

confidence: 99%

“…54) for determination of concentration of fermentation products and ammonia-N (NH 3 -N). VFA were determined using gas chromatography (0.25 · 0.32, id of 0.3 m WCOT Fused Silica Capillary, CHROMPACK CP 9002, Model CP-9002, Delft, The Netherlands) according to Mohammadzadeh et al (2012). Lactic acid concentration was determined spectrophotometrically according to Barker and Summerson (1941), as modified by Pennington and Sutherland (1956).…”

Section: Analytical Proceduresmentioning

confidence: 99%

“…The containers were covered with two layers of sterile cheesecloth to minimise drying. The containers were kept at room temperature and the temperatures were measured at 2-h intervals (Mohammadzadeh et al 2012). Water extracts were obtained from silages just before exposing to air, for enumerating microbial population according to Adesogan and Salawu (2004).…”

Section: Aerobic-stability Measurement and Microbiological Analysis Amentioning

confidence: 99%

“…Moreover, late planting date and early falling in ambient temperature may result in situations where corn is killed by frost. Frosting reduces the level of LAB on the standing plant and elevates numbers of spoilage organisms (Mohammadzadeh et al 2012) and then may produce silages with restricted fermentation and low aerobic stability.…”

Section: Introductionmentioning

confidence: 99%

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Ensiling corn silage with different levels of a multi-species lactic acid bacteria inoculant

2014

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Abstract.A multi-species lactic acid bacterial inoculant (Lactisil maize, LM) was applied to whole-crop corn at different maturities in laboratory silos, to evaluate its effects on biochemical characteristics and aerobic stability. The corn crop was harvested at hard dough (HD, 253.1 g/DM kg), one-third milkline (ML, 293.7 g/DM kg) and one-third milkline with a killing frost (MLF, 297.6 g/DM kg). Crops were chopped to a 2.5-cm theoretical cut length, subsampled and treated with two levels of inoculant (LB1 = 1.5 · 10 5 cfu/g forage, LB2 = 3 · 10 5 cfu/g forage) or untreated (WO). The chemical composition of MLF crops was very similar to that of ML crops. However, lower (P < 0.01) numbers of lactic acid bacteria and higher numbers of yeast were enumerated in MLF than in ML crops. Higher percentages of DM and neutral detergent fibre and higher pH, but lower (P < 0.01) concentrations of water soluble carbohydrate and crude protein were measured in ML and MLF crops than in HD crops. Application of the inoculant increased (P < 0.01) concentrations of volatile fatty acids, neutral detergent fibre and acid detergent fibre in silages. Lactic acid concentration increased (P < 0.01) in HD treatments with an increasing level of inoculant. In contrast, the highest (P < 0.01) lactic acid concentration was measured in LB1 treatment compared with WO and LB2 in ML and MLF silages. Silages prepared from ML and MLF crops had higher (P < 0.01) lactic and acetic acid concentrations but lower (P < 0.01) butyric acid concentrations than did those prepared from HD. The pH in LB1 and LB2 silages was higher (P < 0.01) than that measured in WO silages. Aerobic stability was not influenced by inoculant treatment but low-DM silages were more (P < 0.01) resistant to spoilage. Frost-killed corn crops had a good potential to produce well fermented silage. Using LM resulted in silages with slightly higher fermentation products but it failed to improve aerobic stability of silage after 120 days of ensiling. These results indicated that inoculation of corn crops with LM for a short-duration ensilage period cannot enhance aerobic stability of silages due to insufficient acetic acid production from lactic acid conversion.Additional keywords: aerobic stability, frost-killed corn, heterofermentative fermentation, homofermentative lactic acid bacteria, Lactobacillus buchneri, maturity stage.

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Section: Discussionmentioning

confidence: 99%

Section: Analytical Proceduresmentioning

confidence: 99%

Section: Aerobic-stability Measurement and Microbiological Analysis Amentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ensiling corn silage with different levels of a multi-species lactic acid bacteria inoculant

2014

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“…In recent years, it was found that improvement of both the efficiency of the anaerobic fermentation and the aerobic stability of silage forage could be achieved through the use of several recently found types of dual purpose inoculants (Mohammadzadeh et al, 2012). The spectrum of bacteria contains, on the one hand, homo-fermentative species merely producing lactic acid; and on the other hand, hetero-fermentative species producing a compound of lactic and acetic acids as well as other by-products (e.g., ethanol and carbon dioxide, among others) (Vlková et al, 2012).…”

Section: Treatment Of Grass Materialsmentioning

confidence: 99%

The effect of biological and chemical additives on the chemical composition and fermentation process of Dactylis glomerata silage

Alba-Mejía

Skládanka

Hilgert-Delgado

et al. 2016

Span. j. agric. res.

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This study was carried out to determine the chemical composition, silage quality and ensilability of ten cocksfoot cultivars using biological and chemical silage additives. The plant material was harvested from the first and second cut, cultivated at the Research Station of Fodder Crops in Vatín, Czech Republic. Wilted forage was chopped and ensiled in mini-silos with 3 replicates per treatment. The treatments were: 1) without additives, used as a control; 2) with bacterial inoculants; and 3) with chemical preservatives. The results indicated that the year factor (2012-2013) influenced significantly the chemical composition of the silage in both cuts. The use of biological inoculants reduced the content of crude fibre and acid detergent fibre; but it did not influence the content of neutral detergent fibre, in comparison with the control silage in both cuts. Furthermore, the application of biological inoculants reduced the concentration of lactic acid (LA) and acetic acid (AA) in contrast to the control silage in the first cut. Moreover, in the second cut the same values tended to be the opposite. Interestingly, ‘Amera’ was the unique variety that presented a high concentration of butyric acid (0.2%) in comparison with other varieties in the first cut. In conclusion, the biological inoculants had a favourable effect on silage fermentation. Notably, only ‘Greenly’ and ‘Starly’ varieties from the first cut; and ‘Greenly’, ‘Sw-Luxor’, and ‘Otello’ varieties from the second cut were appropriate for ensiling because their pH-values; LA and AA concentrations were ideal according to the parameters of the fermentation process.

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Inoculation of corn silage with Lactobacillus plantarum and Bacillus subtilis associated with amylolytic enzyme supply at feeding. 1. Feed intake, apparent digestibility, and microbial protein synthesis in wethers

Lara

Bragiato

Rabêlo

et al. 2018

Animal Feed Science and Technology

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The current study aimed to investigate the effects of corn silage inoculation and dietary supplementation with amylolytic enzymes, and their interactions, on feed intake, apparent digestibility, and microbial protein synthesis in wethers. Whole-crop corn forage was ensiled either with water (untreated) or with Lactobacillus plantarum MA 18/5U at 1 × 10 5 cfu/g fresh forage combined with Bacillus subtilis AT553098 at 1 × 10 5 cfu/g fresh forage (inoculated). Eight ruminally cannulated Dorper × Santa Inês crossbred wethers were used in a double 4 × 4 Latin square design. Wethers were assigned to one of four diets, as follows: 1) untreated corn silage with no amylolytic enzyme supply (US-NE); 2) untreated corn silage with amylolytic enzyme supply (US-WE); 3) inoculated corn silage with no amylolytic enzyme supply (IS-NE); and 4) inoculated corn silage with amylolytic enzyme supply (IS-WE). α-Amylase (602 dextrinizing units/kg of dry matter (DM)) was supplied to the wethers at total mixed ration delivery. Dietary supplementation with amylolytic enzyme increased (P = 0.045) DM and starch (P = 0.014) intake in wethers fed the US diet (+245 and +136 g/d, respectively), but not in those fed the IS diet. Inoculation of corn silage (P = 0.026) improved organic matter (OM), crude protein (CP), neutral detergent fiber (aNDFom), and gross energy (GE) digestibility by 5.3%, 11.5%, 25.8%, and 7.1%, respectively. Likewise, enzyme supply increased (P = 0.042) the apparent digestibility of DM, OM, CP, aNDFom, and GE by 5.1%, 4.7%, 9.4%, 20.7%, and 6.2%, respectively. Enzyme supply increased the molar proportion of propionic acid (P = 0.002; 24.1 vs. 19.6 mM / 100 mM) in wethers fed the US diet, but not in those fed the IS diet. Enzyme supply decreased microbial N synthesis (P = 0.025; 6.33 vs. 8.05 g/d) when the wethers were fed the IS diet, but not the US diet. In conclusion, despite the improved OM digestibility, dietary supplementation

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Frosted corn silage with or without bacterial inoculants in dairy cattle ration

Cited by 20 publications

References 36 publications

Ensiling corn silage with different levels of a multi-species lactic acid bacteria inoculant

Ensiling corn silage with different levels of a multi-species lactic acid bacteria inoculant

The effect of biological and chemical additives on the chemical composition and fermentation process of Dactylis glomerata silage

Inoculation of corn silage with Lactobacillus plantarum and Bacillus subtilis associated with amylolytic enzyme supply at feeding. 1. Feed intake, apparent digestibility, and microbial protein synthesis in wethers

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