Frozen and cold-stored platelets: reconsidered platelet products

Jimenez‐Marco, Teresa; Castrillo, Azucena; Hierro-Riu, Fernando; Vicente, Vicente; Rivera, José

doi:10.1080/09537104.2021.1967917

Cited by 16 publications

(23 citation statements)

References 92 publications

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“…Patients requiring massive transfusion make up for the minority of total blood products demand in most civilian hospitals, particularly in developed countries, as many other transfusion indications like non-acute controlled hemorrhage, sickle cell disease, homozygous beta thalassemia, symptomatic anemia, and myelodysplastic syndrome usually require one type of component. Also, in the future WB may become less important when new developments that improve shelf life and logistics (i.e., dried plasma and cold stored PLT), and thus the early availability of these components beside RBCs in the (pre)-hospital setting 78,79 …”

Section: Discussionmentioning

confidence: 99%

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Whole blood transfusion in the treatment of acute hemorrhage, a systematic review and meta-analysis

Horst

Rijnhout

Noorman³

et al. 2023

J Trauma Acute Care Surg

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BACKGROUND Whole blood (WB) transfusion received renewed interest after recent armed conflicts. The effectiveness as compared with blood component transfusion (BCT) is, however, still topic of debate. Therefore, this study investigated the effect of WB ± BCT as compared with BCT transfusion on survival in trauma patients with acute hemorrhage. METHODS Studies published up to January 16, 2023, including patients with traumatic hemorrhage comparing WB ± BCT and BCT were included in meta-analysis. Subanalyses were performed on the effectiveness of WB in the treatment of civilian or military trauma patients, patients with massive hemorrhage and on platelet (PLT)/red blood cell (RBC), plasma/RBC and WB/RBC ratios. Methodological quality of studies was interpreted using the Cochrane risk of bias tool. The study protocol was registered in PROSPERO under number CRD42022296900. RESULTS Random effect pooled odds ratio (OR) for 24 hours mortality in civilian and military patients treated with WB as compared with BCT was 0.72 (95% confidence interval [CI], 0.53–0.97). In subanalysis of studies conducted in civilian setting (n = 20), early (4 hours, 6 hours, and emergency department) and 24 hours mortality was lower in WB groups compared with BCT groups (OR, 0.65; 95% CI, 0.44–0.96 and OR, 0.71; 95% CI, 0.52–0.98). No difference in late mortality (28 days, 30 days, in-hospital) was found. In military settings (n = 7), there was no difference in early, 24 hours, or late mortality between groups. The WB groups received significant higher PLT/RBC (p = 0.030) during early treatment and significant higher PLT/RBC and plasma/RBC ratios during 24 hours of treatment (p = 0.031 and p = 0.007). The overall risk of bias in the majority of studies was judged as serious due to serious risk on confounding and selection bias, and unclear information regarding cointerventions. CONCLUSION Civilian trauma patients with acute traumatic hemorrhage treated with WB ± BCT as compared to BCT had lower odds on early and 24-hour mortality. In addition, WB transfusion resulted in higher PLT/RBC and plasma/RBC ratios. LEVEL OF EVIDENCE Systematic Review and Meta-Analysis; Level III.

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Section: Discussionmentioning

confidence: 99%

“…Also, in the future WB may become less important when new developments that improve shelf life and logistics (i.e., dried plasma and cold stored PLT), and thus the early availability of these components beside RBCs in the (pre)-hospital setting. 78,79…”

Section: Discussionmentioning

confidence: 99%

Whole blood transfusion in the treatment of acute hemorrhage, a systematic review and meta-analysis

Horst

Rijnhout

Noorman³

et al. 2023

J Trauma Acute Care Surg

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“…The standard technical procedure for the conservation of platelets from PRP includes gentle agitation in proper aseptic containers at room temperature of 20-24 °C [ 5 , 6 ]. Under these conditions, storage is limited to 5 days since bacterial growth is favored [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative and Fibrinolytic Mechanisms: Two Important Processes to Consider in Platelet Storage

García-Bañuelos¹,

Galicia-Moreno²,

Ramirez³

et al. 2023

Tjh

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Objective: Platelet-rich plasma (PRP) is used in multiple coagulation disorders. Its therapeutic effectiveness relies on technical procedures related to PRP procurement and preservation because free radicals induce platelet activation and aging. This work aims to elucidate the oxidative mechanisms involved in activation of platelets obtained from PRP during storage. Materials and Methods: One hundred ten PRP batches were obtained from healthy donors and kept under stirring at a temperature of 20-24 °C. Protein extraction was performed from platelet homogenates and plasma at different times of storage from day 1 to 20. The activities of antioxidant markers such as catalase (CAT), superoxide dismutase, and ceruloplasmin, as well as fibrinolytic protein activity metalloproteases 2 and 3, plasmin, and urokinase plasminogen activator, were analyzed by zymography assays. Oxidized proteins were also determined. Results: Significant activity of antioxidant enzymes and fibrinolytic molecules was observed on day 5 of storage in PRP homogenates, which increased over time and was concomitantly correlated with oxidized protein levels. Reverse enzymatic activity patterns were observed in plasma, except for CAT, which remained unchanged. Conclusion: Storage conditions of platelets from PRP for up to 5 days induced in vitro platelet activation by oxidative damage and proteolysis. This finding confirms the need for proper management of these blood products to preserve their viability and functionality.

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“…Introducing this PI step in PCs production, enables to increase the storage duration from 5 to 7 days at 22°C. However, the PI process, 1–8 storage conditions, and duration 9–13 modify platelet functions 14 …”

Section: Introductionmentioning

confidence: 99%

“…Introducing this PI step in PCs production, enables to increase the storage duration from 5 to 7 days at 22°C. However, the PI process, [1][2][3][4][5][6][7][8] storage conditions, and duration [9][10][11][12][13] modify platelet functions. 14 Understanding the in vivo impact of transfused platelets on patients is then primordial.…”

Section: Introductionmentioning

confidence: 99%

Two novel platelet biotinylation methods and their impact on stored platelet concentrates in a blood bank environment

et al. 2022

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Background: Storage of platelet concentrates (PCs) has an impact on platelet quality and possibly affects their functions after transfusion. The influence of processing and storage conditions of PCs on their in vivo function upon transfusion is unknown. One option for investigating this question is to implement an ex vivo labeling of human platelets, to analyze them after transfusion into heathy volunteers and/or patients. In this study, we developed two labeling methods employing biotin. Methods: Two methods of biotinylation were compared to a control (standard PC). The "Bio-Wash" process used washing steps to label all platelets within the PC; for the other method, "Bio-Direct," one fifth of the PC were directly labeled without washing steps. The control and the two biotinylated PCs were analyzed over 7 days of storage. Labeling efficiency, platelet counts, phenotypes, and functions, along with time and costs, were evaluated to select the best process.Results: Both methods achieved a stable labeling through the storage, with similar platelet counts and metabolism in comparison to control PCs. Bio-Wash showed higher activation phenotype and lower aggregation response in comparison to the Bio-Direct method. The Bio-Direct was performed within 1.5 h versus 3 h for the Bio-Wash. However, the Bio-Direct required 12 mg of biotin instead of 8 mg for the other process. Conclusion: We set up two methods of biotinylation that can be easily implemented in a blood bank environment. The Bio-Direct process was preferred to the Bio-Wash because of its similarity, from a functional and phenotypic point of view, with standard PCs.

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Frozen and cold-stored platelets: reconsidered platelet products

Cited by 16 publications

References 92 publications

Whole blood transfusion in the treatment of acute hemorrhage, a systematic review and meta-analysis

Whole blood transfusion in the treatment of acute hemorrhage, a systematic review and meta-analysis

Oxidative and Fibrinolytic Mechanisms: Two Important Processes to Consider in Platelet Storage

Two novel platelet biotinylation methods and their impact on stored platelet concentrates in a blood bank environment

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