Frozen Semen Characteristics of Limousin Bull at Different Ages

,; Baharun, A.; Setiawan, A. B.; ,; Rahmi, A.; ,; Iskandar, H.; ,; Gunawan, M.; ,; Anwar, S.; ,; Maulana, T.; ,; Kaiin, E. M.; ,; Said, S.; ,

doi:10.5398/tasj.2023.46.3.306

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…The results of whole plasma membrane analysis showed that Pasundan bulls named Bagaskara and Kartarajasa had lower values ( p < 0.05) compared to other males ( Table 1 ). Baharun et al [18] reported that an IPM plays an essential role in metabolic processes associated with spermatozoa motility and viability, and it is essential for fertilization, fertility, and spermatozoa function for successful AI. Impairment to the plasma membrane can diminish sperm motility, alter spermatozoa morphology and metabolism, and cause an imbalance of intracellular components such that spermatozoa fertility may be compromised.…”

Section: Resultsmentioning

confidence: 99%

Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Baharun,

Rahmi,

Handarini

et al. 2024

J Adv Vet Anim Res

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Objective: To determine the correlation between the molecular weight (MW) of proteins in seminal plasma and spermatozoa and the quality of fresh and frozen semen production in Pasundan bulls. Materials and methods: Nine selected Pasundan bulls, aged 5–10 years, from the Regional Artificial Insemination Center at Ciamis, West Java, Indonesia, were used in the study, with fresh semen sperm motility ≥70% and <70%. We analyzed the motility, viability, integrity of the intact plasma membrane (IPM), and the morphological characteristics of spermatozoa. 1D-SDS-PAGE analysis was performed to determine the protein profile by assessing MW, depicted as bands on the gel. Results: The motility, viability, and IPM of spermatozoa had lower values (p < 0.05) in Pasundan bulls named Bagaskara and Kertarajasa compared to the other bulls. Proteins with MW 35–50 kDa were not detected in the seminal plasma of Pasundan bulls, exhibiting low quality in fresh semen. The correlation analysis showed that the non-detected proteins with MW 35–50 kDa in seminal plasma correlated with spermatozoa motility (r = 0.421), viability (r = 0.424), and IPM (r = 0.428) so that fresh semen quality was low in both Pasundan bulls. Analysis of semen volume, spermatozoa concentration, and spermatozoa motility showed that the average frozen semen production of Pasundan bulls per ejaculate was 128.73 ± 15.35 straws. Conclusion: Protein analysis based on MW is a predictive indicator for the quality of fresh semen and the production of frozen semen in Pasundan bulls. Evaluation parameters of fresh semen quality by MW analysis can be used to select Pasundan bulls in Indonesia.

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Section: Resultsmentioning

confidence: 99%

Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Baharun,

Rahmi,

Handarini

et al. 2024

J Adv Vet Anim Res

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Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility

Hasbi,

Iskandar,

Sonjaya

et al. 2024

Vet World

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Background and Aim: Fertility is crucial for enhancing the efficiency of livestock production, as it directly impacts the reproductive rates. A comprehensive understanding of the relationship between sperm quality and embryo development is key to optimizing reproductive outcomes and improving the quality of livestock. This study analyzed the developmental competence of in vitro embryos recovered from Bali cattle with normal or poor sperm motility. Materials and Methods: Nine bulls with normal fresh semen (NFS) or poor fresh semen (PFS) motility were ejaculated for semen. Semen ejaculates, including volume, motility, and sperm concentration, were evaluated immediately after collection to measure the quality of the fresh semen. Frozen semen was evaluated using computer-assisted semen analysis (CASA) for motility, progressive sperm motility, distance curve path, distance curve linear, distance straight line, average path velocity, curvilinear velocity, linear velocity, straightness (STR), linearity of forward progression (LIN), wobble, and average lateral head displacement (ALH). Bull groups were used to determine in vitro embryo cleavage ability after fertilization of Bali cattle. Ovaries of Bali cattle were collected by slicing, and only cytoplasmic oocytes with compact cumulus cells were used in this study. The oocytes were matured, and in vitro fertilization was performed using fertilization media with a final sperm concentration of 1.5 Ã— 106 spermatozoa/mL. After 48 h, the embryo cleavage ability of the cultured oocytes was evaluated. Results: There were significant differences in motility values between the NFS and PFS groups; however, there were no significant differences in the volume or sperm concentration. There was a significant difference in the LIN value between the groups but no significant differences in other CASA parameters. There were no significant differences in the cleavage rate and morula between the groups, but a positive correlation was observed between the cleavage rate and the morula and between the morula and ALH. A significant negative correlation was observed between the cleavage rate and STR and between the morula and STR; no significant differences were observed for other variables. Conclusion: Despite variations in sperm characteristics, both normal and poor sperm motility demonstrated comparable in vitro embryonic development competence. These findings provide important insights into the fertility potential of Bali bulls, providing valuable information that can enhance selection strategies to improve the quality of livestock production. Keywords: Bali cattle, embryos, in vitro semen quality.

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Reproductive Performance of Crossbreed Cows on Lowland Terrain

Ardiyansyah,

Sri Agus Sudjarwo,

Nusdianto Triakoso

et al. 2023

Ovz

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This study aims to identify the differences in reproduction performance of Ongole cross (PO), Limousine cross (Limpo), and Simmental cross (Simpo) in Kerek district, Tuban regency. Primary data was taken by interviewing inseminators and farmers, while secondary data was taken from the Fisheries and Animal Husbandry Department of Tuban regency. The total sample was 360 cows consisting of 30 PO, Limpo, and Simpo cows aged 3, 4, 5, and 6 years respectively which were calculated based on the Roscoe formula. Data was analyzed using the Simple Linear Regression and ANOVA followed by Duncan's multiple range test at a significance level of 95%. The results showed that the services per conception (S/C), conception rate (CR), and calving rate (CvR) were not significantly different (p >0.05) between PO, Limpo and Simpo cows. Meanwhile, days open (DO) and fertility status (FS) of PO cows were higher (p <0.05) than Limpo and Simpo cows. There was no significant difference (p >0.05) in DO and FS between Limpo and Simpo cows. The linear equation with age as a predictor (X) of S/C, DO, and CI (Y) values were not significant (p >0.05) in PO, Limpo, and Simpo cows. It could be concluded that the reproductive performance of PO cows was better than Limpo and Simpo cows.

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Frozen Semen Characteristics of Limousin Bull at Different Ages

Cited by 3 publications

References 41 publications

Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility

Reproductive Performance of Crossbreed Cows on Lowland Terrain

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