Frozen storage stimulates the formation of embryo-like structures and elongating shoots in explants from mature Larix decidua and L. x eurolepsis trees

Bonga, J. M.

doi:10.1007/bf00034841

Cited by 21 publications

(12 citation statements)

References 14 publications

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“…(Smith, 1996), and shoot explants was mostly nonembryogenic callus formation, and elongation in shoot explants on media without plant growth regulators. Formation of embryo-like structures on frozen storage shoot explants, as described for Larix (Bonga, 1996), was not observed in sugi shoots.…”

mentioning

confidence: 64%

Embryogenic Cell Culture, Protoplast Regeneration, Cryopreservation, Biolistic Gene Transfer and Plant Regeneration in Japanese Cedar (Cryptomeria japonica D. Don).

Maruyama

Tanaka

Hosoi

et al. 2000

Plant Biotechnology

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Somatic embryogenesis in Cryptomeria japonica was initiated at a relatively high frequency from immature seeds collected from the end of June to mid -July. Induction of embryogenic cultures was possible on media with or without plant growth regulators, and the initiation frequency varied from 5 to 16%. Embryogenic cell lines have been maintained and proliferated for more than 2 years in solid and liquid media. For long-term storage embryogenic cells were cryopreserved using a simple freezing method. Cotyledonary embryos were obtained mostly on maturation media containing abscisic acid (ABA) and polyethylene glycol (PEG) as osmotic agent, however, the plant conversion rate was still low. Plants regenerated from somatic embryos continued growing in a greenhouse. Furthermore, a procedure for the individual culture of protoplasts isolates from embryonal masses, and an approach for microprojectile bombardment-mediated transformation using pIPT and pMAT vectors was also described.

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mentioning

confidence: 64%

Embryogenic Cell Culture, Protoplast Regeneration, Cryopreservation, Biolistic Gene Transfer and Plant Regeneration in Japanese Cedar (Cryptomeria japonica D. Don).

Maruyama

Tanaka

Hosoi

et al. 2000

Plant Biotechnology

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“…Explants from adult trees: success has been achieved in Larix decidua, Larix eurolepis (Bonga 1997). In these experiments embryogenic tissues have been obtained on explants from adult 42-years old trees.…”

Section: Initiation Of Somatic Embryogenesismentioning

confidence: 99%

Conifer somatic embryogenesis – an efficient plant regeneration system for theoretical studies and mass propagation

2015

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Abstract:Since the first report of somatic embryogenesis in Norway spruce in 1985, the in vitro process has been initiated for a number of conifer species belonging to different genera. The process of somatic embryogenesis involves initiation, proliferation, maturation and plantlets (emblings) regeneration. The initiation of somatic embryogenesis is restricted mostly to juvenile explants, although recently explants taken from adult trees produced embryogenic tissues. The successful initiation, maturation and emblings regeneration are affected by factors as developmental stage of primary explants, genotype, plant growth regulators content in the culture medium, light conditions. Optimisation of mentioned factors resulted in regeneration of emblings capable of growing after transfer to soil.

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“…This technique enables the mother plants genetic features to be conserved maximally in the regenerated plants van Staden, 2003, 2005). Initiation and establishment of embryogenic cultures from vegetative tissues of mature conifers has been attempted in a few species von Aderkas, 1988, 1993;Bonga and Pond, 1991;Westcott, 1994;Bonga, 1995Bonga, , 1996Bonga, , 2004Smith, 1997). In conifers synthetic seed production is well established using mature somatic embryos (Attree and Fowke, 1993;Lulsdorf et al, 1993;Attree et al, 1994) without in vitro storage for commercialization.…”

Section: Introductionmentioning

confidence: 99%

Storability and germination of sodium alginate encapsulated somatic embryos derived from the vegetative shoot apices of mature Pinus patula trees

Malabadi

Staden

2005

Plant Cell Tiss Organ Cult

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Storability and germination of sodium alginate encapsulated somatic embryos derived from vegetative shoot apices of mature Pinus patula trees were tested on half strength DCR basal medium without growth regulators. The germination percentage of encapsulated somatic embryos was affected significantly by the concentration of sodium alginate and the duration of exposure to calcium chloride. Somatic embryos encapsulated with 2.5% sodium alginate dissolved in DCR basal salts gave significantly higher germination (89%) than other treatments. Short (5 min) incubation of the alginate encapsulated embryos in calcium chloride solution proved to be the best encapsulation procedure and the embryos subsequently gave the highest germination (89%). Synthetic seeds could be stored at 2°C for 120 days without a reduction in germination as opposed to non-encapsulated somatic embryos which showed only 9% germination after 20 days at 2°C. Germinated synthetic seeds produced normal plantlets. This study reports for the first time the storability of encapsulated somatic embryos generated from vegetative shoot apices of mature Pinus patula trees. This has potential for application in forestry.

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Frozen storage stimulates the formation of embryo-like structures and elongating shoots in explants from mature Larix decidua and L. x eurolepsis trees

Cited by 21 publications

References 14 publications

Embryogenic Cell Culture, Protoplast Regeneration, Cryopreservation, Biolistic Gene Transfer and Plant Regeneration in Japanese Cedar (Cryptomeria japonica D. Don).

Embryogenic Cell Culture, Protoplast Regeneration, Cryopreservation, Biolistic Gene Transfer and Plant Regeneration in Japanese Cedar (Cryptomeria japonica D. Don).

Conifer somatic embryogenesis – an efficient plant regeneration system for theoretical studies and mass propagation

Storability and germination of sodium alginate encapsulated somatic embryos derived from the vegetative shoot apices of mature Pinus patula trees

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