Frozen/Thawed Platelets: Importance of Osmotic Tolerance and Platelet Subpopulations

Arnaud, Françoise

doi:10.1006/cryo.1998.2138

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…A cooling rate that is too fast results in an insufficient release of water by the platelets, resulting in the formation of intracellular ice crystals [17]. The optimal gradient for freezing platelets ranges in a broad spectrum from 1 to 20 °C/min [18].…”

Section: Introductionmentioning

confidence: 99%

Comparison of various dimethylsulphoxide‐containing solutions for cryopreservation of leucoreduced platelet concentrates

et al. 2003

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Section: Introductionmentioning

confidence: 99%

Comparison of various dimethylsulphoxide‐containing solutions for cryopreservation of leucoreduced platelet concentrates

et al. 2003

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“…For any specific bio-system cryopreservation, there is an optimal cooling rate. The optimal cooling-rate range for platelets freezing is from 1 to 258C/min, with the use of various cryoprotectants including Me 2 SO, propylene glycol, glycerol, and dextran T40 (Arnaud, 1999;Brodthagen et al, 1985). Our optimal cooling rate for platelets freezing with trehalose and phosphate ion is between 1 and 58C/min.…”

Section: Discussionmentioning

confidence: 99%

Platelet cryopreservation using a trehalose and phosphate formulation

Nie

Pablo

Palecek

2005

Biotechnol. Bioeng.

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Long-term storage of platelets is infeasible due to platelet activation at low temperatures. In an effort to address this problem, we evaluated the effectiveness of a formulation combining trehalose and phosphate in protecting platelet structure and function following cryopreservation. An annexin V binding assay was used to quantify the efficacy of the trehalose and phosphate formulation in suppressing platelet activation during cryopreservation. Of the platelets cryopreserved with the trehalose plus phosphate formulation, 23% +/- 1.2% were nonactivated, compared with 9.8% +/- 0.26% nonactivated following cryopreservation with only trehalose. The presence of both trehalose and phosphate in the cryopreservation medium is critical for cell survival and preincubation in trehalose plus phosphate solutions further enhances viability. The effectiveness of trehalose plus phosphate in preserving platelets in a nonactivated state is comparable to 6% dimethyl sulfoxide (Me(2)SO). Measurements of platelet metabolic activity using an alamarBlue assay also established that trehalose plus phosphate is superior to trehalose alone. Finally, platelets protected by the trehalose plus phosphate formulation exhibit similar aggregation response upon thrombin addition as fresh platelets, but an increase of cytosolic calcium concentration upon thrombin addition was not observed in the cryopreserved platelets. These results suggest that trehalose and phosphate protect several aspects of platelet structure and function during cryopreservation, including an intact plasma membrane, metabolic activity, and aggregation in response to thrombin, but not intracellular calcium release in response to thrombin.

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“…Notably, most recent studies indicate that cryopreserved and cold platelets display a pro-coagulant profile that produces a rapid hemostatic response, which is needed in actively bleeding patients [ 79 ]. Despite this, it should be expected that collected platelets in a frozen unit are heterogeneous and thus contain subpopulations that can be more sensitive to freezing [ 80 ]. In light of this, approaches that reduce levels of calcium, which is known to cause cryopreservation-induced platelet damage, through the use of a calcium chelator prior to preservation to limit the amount of damage that platelets undergo while freezing [ 81 ].…”

Section: Alternatives To Room Temperature Storagementioning

confidence: 99%

Bacterial Contamination of Platelet Products

Jacobs,

Zhou,

Tayal

et al. 2024

Microorganisms

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Transfusion of bacterially contaminated platelets, although rare, is still a major cause of mortality and morbidity despite the introduction of many methods to limit this over the past 20 years. The methods used include improved donor skin disinfection, diversion of the first part of donations, use of apheresis platelet units rather than whole-blood derived pools, primary and secondary testing by culture or rapid test, and use of pathogen reduction. Primary culture has been in use the US since 2004, using culture 24 h after collection of volumes of 4–8 mL from apheresis collections and whole-blood derived pools inoculated into aerobic culture bottles, with limited use of secondary testing by culture or rapid test to extend shelf-life from 5 to 7 days. Primary culture was introduced in the UK in 2011 using a “large-volume, delayed sampling” (LVDS) protocol requiring culture 36–48 h after collection of volumes of 16 mL from split apheresis units and whole-blood derived pools, inoculated into aerobic and anaerobic culture bottles (8 mL each), with a shelf-life of 7 days. Pathogen reduction using amotosalen has been in use in Europe since 2002, and was approved for use in the US in 2014. In the US, recent FDA guidance, effective October 2021, recommended several strategies to limit bacterial contamination of platelet products, including pathogen reduction, variants of the UK LVDS method and several two-step strategies, with shelf-life ranging from 3 to 7 days. The issues associated with bacterial contamination and these strategies are discussed in this review.

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Frozen/Thawed Platelets: Importance of Osmotic Tolerance and Platelet Subpopulations

Cited by 6 publications

References 30 publications

Comparison of various dimethylsulphoxide‐containing solutions for cryopreservation of leucoreduced platelet concentrates

Comparison of various dimethylsulphoxide‐containing solutions for cryopreservation of leucoreduced platelet concentrates

Platelet cryopreservation using a trehalose and phosphate formulation

Bacterial Contamination of Platelet Products

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