Fructan Enzymes in Microbes and Plants: Structure, Function, and Product Formation

Alamäe, Tiina; Ernits, Karin; Hernández, Lázaro; Visnapuu, Triinu; Ende, Wim Van den

doi:10.1016/b978-0-323-85410-8.00018-1

Cited by 6 publications

(3 citation statements)

References 193 publications

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“…The same effect may not be generated by levan since the endo‐levanase from B. paralicheniformis LB1‐1A may be ineffective in degrading the high‐DP branched levan produced by the levansucrase of Pseudomonas syringae (15% branching) that was used in this study. Alternatively, adding exogenous levan to diverse types of Bacillaceae may exert differential stabilizing effects, under circumstances where they may not be able to produce their own levan, since sucrose levels in the apoplast may be below the threshold level required to initiate the transferase activity of GH68 enzymes (Alamäe et al, 2023; Morvan‐Bertrand et al, 2023). Therefore, it would be interesting to consider the effect of adding sucrose to these mixtures as well.…”

Section: Discussionmentioning

confidence: 99%

“…3.2.1.7) are involved in degradation of levan and inulin to levan oligosaccharides (LOS) and inulin oligosaccharides (IOS), respectively, usually in concert with exo‐type fructanases (E.C. 3.2.1.80) (Arrieta et al, 2004; Porras‐Domínguez et al, 2014; Mardo et al, 2017; Abaramak et al, 2021; Nasir et al, 2022; Alamäe et al, 2023). Besides other exopolymers, these microbes also incorporate fructans as extracellular carbohydrate polymers in their outer layers, serving as protective compounds and/or as (temporal) carbohydrate reserves (Dogsa et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Exploring Synergistic Effects of Levan and Levan‐MetabolizingBacillaceae in Promoting Growth and Enhancing Immunity of Tomato and Wheat

Jiao,

Tran‐Minh,

Nasir

et al. 2024

Physiologia Plantarum

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Boosting plant immunity by priming agents can lower agrochemical dependency in plant production. Levan and levan‐derived oligosaccharides (LOS) act as priming agents against biotic stress in several crops. Additionally, beneficial microbes can promote plant growth and protect against fungal diseases. This study assessed possible synergistic effects caused by levan, LOS and five levan‐ and LOS‐metabolizing Bacillaceae (Bacillus and Priestia) strains in tomato and wheat. Leaf and seed defense priming assays were conducted in non‐soil (semi‐sterile substrate) and soil‐based systems, focusing on tomato‐Botrytis cinerea and wheat‐Magnaporthe oryzae Triticum (MoT) pathosystems. In the non‐soil system, seed defense priming with levan, the strains (especially Bacillus velezensis GA1), or their combination significantly promoted tomato growth and protection against B. cinerea. While no growth stimulatory effects were observed for wheat, disease protective effects were also observed in the wheat‐MoT pathosystem. When grown in soil and subjected to leaf defense priming, tomato plants co‐applied with levan and the bacterial strains showed increased resistance to B. cinerea compared with plants treated with levan or single strains, and these effects were synergistic in some cases. For seed defense priming in soil, more synergistic effects on disease tolerance were observed in a non‐fertilized soil as compared to a fertilized soil, suggesting that potential prebiotic effects of levan are more prominent in poor soils. The potential of using combinations of Bacilliaceae and levan in sustainable agriculture is discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploring Synergistic Effects of Levan and Levan‐MetabolizingBacillaceae in Promoting Growth and Enhancing Immunity of Tomato and Wheat

Jiao,

Tran‐Minh,

Nasir

et al. 2024

Physiologia Plantarum

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“…β-Fructofuranosidases are part of the family glycoside hydrolase 32 (GH32) enzymes and are well-known for their ability to hydrolyze sucrose (Suc), as well as fructose (Fru)-based oligo- and polysaccharides derived from Suc, termed fructans . Inulin-type fructans are the best-known and widely applied type of fructans, used as health-promoting prebiotics in the food industry, but they are also emerging as priming agents in protection of lettuce against gray mold infections through leaf spraying. , Although prebiotics are mainly known from the animal/human gut context, a theoretical approach in which prebiotic molecules promote beneficial microorganisms in the context of plant protection has been proposed .…”

Section: Introductionmentioning

confidence: 99%

The Complex GH32 Enzyme Orchestra from Priestia megaterium Holds the Key to Better Discriminate Sucrose-6-phosphate Hydrolases from Other β-Fructofuranosidases in Bacteria

Dobrange,

Porras-Domínguez,

Van den Ende

2024

J. Agric. Food Chem.

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Inulin is widely used as a prebiotic and emerging as a priming compound to counteract plant diseases. We isolated inulin-degrading strains from the lettuce phyllosphere, identified as Bacillus subtilis and Priestia megaterium, species hosting wellknown biocontrol organisms. To better understand their varying inulin degradation strategies, three intracellular βfructofuranosidases from P. megaterium NBRC15308 were characterized after expression in Escherichia coli: a predicted sucrose-6-phosphate (Suc6P) hydrolase (SacAP1, supported by molecular docking), an exofructanase (SacAP2), and an invertase (SacAP3). Based on protein multiple sequence and structure alignments of bacterial glycoside hydrolase family 32 enzymes, we identified conserved residues predicted to be involved in binding phosphorylated (Suc6P hydrolases) or nonphosphorylated substrates (invertases and fructanases). Suc6P hydrolases feature positively charged residues near the structural catalytic pocket (histidine, arginine, or lysine), whereas other β-fructofuranosidases contain tryptophans. This correlates with our phylogenetic tree, grouping all predicted Suc6P hydrolases in a clan associated with genomic regions coding for transporters involved in substrate phosphorylation. These results will help to discriminate between Suc6P hydrolases and other β-fructofuranosidases in future studies and to better understand the interaction of B. subtilis and P. megaterium endophytes with sucrose and/or fructans, sugars naturally present in plants or exogenously applied in the context of defense priming.

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The application of fructosyltransferase in model solutions to reduce sucrose content and synthesize short-chain fructooligosaccharides

Cywińska-Antonik,

Szczepańska-Stolarczyk,

Marszałek

2024

Food Bioscience

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Fructan Enzymes in Microbes and Plants: Structure, Function, and Product Formation

Cited by 6 publications

References 193 publications

Exploring Synergistic Effects of Levan and Levan‐MetabolizingBacillaceae in Promoting Growth and Enhancing Immunity of Tomato and Wheat

Exploring Synergistic Effects of Levan and Levan‐MetabolizingBacillaceae in Promoting Growth and Enhancing Immunity of Tomato and Wheat

The Complex GH32 Enzyme Orchestra from Priestia megaterium Holds the Key to Better Discriminate Sucrose-6-phosphate Hydrolases from Other β-Fructofuranosidases in Bacteria

The application of fructosyltransferase in model solutions to reduce sucrose content and synthesize short-chain fructooligosaccharides

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