Fructan exohydrolases (<scp>FEHs</scp>) are upregulated by salicylic acid together with defense‐related genes in non‐fructan accumulating plants

Nguyen, Thi Ngoc Hanh; Leclerc, Laëtitia; Manzanares‐Dauleux, Maria J.; Gravot, Antoine; Vicré, Maïté; Morvan‐Bertrand, Annette; Prud'homme, Marie‐Pascale

doi:10.1111/ppl.13975

Cited by 1 publication

(1 citation statement)

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“…UPL3, previously recognized for its pleiotropic regulatory roles, influences elements such as flavonoid synthesis, seed maturation, hormone-induced development, and stress response, by targeting related transcription factors [ 81 – 83 ]. We report the presence of the ethylene-responsive component, Hevein-like preproprotein (HEL) [ 84 ], in R4, which aligns with prior research findings that highlighted UPL3’s role in shutting down the EIN3-induced transcriptional cascade. Similarly, the down-regulation of UPL3 should restore the NPR1-mediated plant immune response.…”

Section: Discussionsupporting

confidence: 80%

Comparative proteomics in tall fescue to reveal underlying mechanisms for improving Photosystem II thermotolerance during heat stress memory

Wang,

et al. 2024

BMC Genomics

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Background The escalating impacts of global warming intensify the detrimental effects of heat stress on crop growth and yield. Among the earliest and most vulnerable sites of damage is Photosystem II (PSII). Plants exposed to recurring high temperatures develop heat stress memory, a phenomenon that enables them to retain information from previous stress events to better cope with subsequent one. Understanding the components and regulatory networks associated with heat stress memory is crucial for the development of heat-resistant crops. Results Physiological assays revealed that heat priming (HP) enabled tall fescue to possess higher Photosystem II photochemical activity when subjected to trigger stress. To investigate the underlying mechanisms of heat stress memory, we performed comparative proteomic analyses on tall fescue leaves at S0 (control), R4 (primed), and S5 (triggering), using an integrated approach of Tandem Mass Tag (TMT) labeling and Liquid Chromatography-Mass Spectrometry. A total of 3,851 proteins were detected, with quantitative information available for 3,835 proteins. Among these, we identified 1,423 differentially abundant proteins (DAPs), including 526 proteins that were classified as Heat Stress Memory Proteins (HSMPs). GO and KEGG enrichment analyses revealed that the HSMPs were primarily associated with the “autophagy” in R4 and with “PSII repair”, “HSP binding”, and “peptidase activity” in S5. Notably, we identified 7 chloroplast-localized HSMPs (HSP21, DJC77, EGY3, LHCA4, LQY1, PSBR and DEGP8, R4/S0 > 1.2, S5/S0 > 1.2), which were considered to be effectors linked to PSII heat stress memory, predominantly in cluster 4. Protein-protein interaction (PPI) analysis indicated that the ubiquitin-proteasome system, with key nodes at UPL3, RAD23b, and UCH3, might play a role in the selective retention of memory effectors in the R4 stage. Furthermore, we conducted RT-qPCR validation on 12 genes, and the results showed that in comparison to the S5 stage, the R4 stage exhibited reduced consistency between transcript and protein levels, providing additional evidence for post-transcriptional regulation in R4. Conclusions These findings provide valuable insights into the establishment of heat stress memory under recurring high-temperature episodes and offer a conceptual framework for breeding thermotolerant crops with improved PSII functionality.

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Section: Discussionsupporting

confidence: 80%