Fructan metabolism in two species of <i>Bromus</i> subjected to chilling and water stress

Puebla, Andrea Fabiana; Salerno, Graciela L.; Pontis, Horacio G.

doi:10.1046/j.1469-8137.1997.00728.x

Cited by 23 publications

(12 citation statements)

References 27 publications

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“…Fructans have been implicated in protecting plants from cold and water stress and in sustaining sucrose levels in the plant (Pilon‐Smits et al . 1995; Puebla, Salerno & Pontis 1997; Hincha et al . 2002; Konstantinova et al .…”

Section: Discussionmentioning

confidence: 99%

“…The occurrence of fructans in the florets at this stage of development has not been reported previously. Fructans have been implicated in protecting plants from cold and water stress and in sustaining sucrose levels in the plant (Pilon-Smits et al 1995;Puebla, Salerno & Pontis 1997;Hincha et al 2002;Konstantinova et al 2002;Pollock et al 2003;Ji et al 2007). Genetic engineering of rice capable of synthesizing fructans has recently been shown to improve chilling tolerance (Kawakami, Sato & Yoshida 2008).…”

Section: Control Of Sink Strength Is Important For Reproductive Stagementioning

confidence: 99%

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Importance of pre‐anthesis anther sink strength for maintenance of grain number during reproductive stage water stress in wheat

Shiran

JianLin

et al. 2010

Plant Cell & Environment

327

254

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Reproductive stage water stress leads to spikelet sterility in wheat. Whereas drought stress at anthesis affects mainly grain size, stress at the young microspore stage of pollen development is characterized by abortion of pollen development and reduction in grain number. We identified genetic variability for drought tolerance at the reproductive stage. Drought-tolerant wheat germplasm is able to maintain carbohydrate accumulation in the reproductive organs throughout the stress treatment. Starch depletion in the ovary of drought-sensitive wheat is reversible upon re-watering and cross-pollination experiments indicate that the ovary is more resilient than the anther. The effect on anthers and pollen fertility is irreversible, suggesting that pollen sterility is the main cause of grain loss during drought conditions in wheat. The difference in storage carbohydrate accumulation in drought-sensitive and droughttolerant wheat is correlated with differences in sugar profiles, cell wall invertase gene expression and expression of fructan biosynthesis genes in anther and ovary (sucrose : sucrose 1-fructosyl-transferase, 1-SST; sucrose : fructan 6-fructosyl-transferase, 6-SFT). Our results indicate that the ability to control and maintain sink strength and carbohydrate supply to anthers may be the key to maintaining pollen fertility and grain number in wheat and this mechanism may also provide protection against other abiotic stresses.

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Section: Discussionmentioning

confidence: 99%

Section: Control Of Sink Strength Is Important For Reproductive Stagementioning

confidence: 99%

Importance of pre‐anthesis anther sink strength for maintenance of grain number during reproductive stage water stress in wheat

Shiran

JianLin

et al. 2010

Plant Cell & Environment

327

254

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“…f., a grass species native to a cold arid region with constitutive fructan synthesis showed greater root survival to desiccation than the closely related Bromus auleticus Trin. ex Nees, which accumulates fructan only when exposed to low temperature (Puebla et al, 1997). Although a direct protective role of fructans remains conjectural, the recent demonstration by Demel et al (1998) of a strong and specific interaction between HMW fructans with model membranes suggests that fructans might help prevent lipid condensation and phase transitions to take place in water stress or freeze‐desiccated cells.…”

Section: Discussionmentioning

confidence: 99%

Freezing Tolerance and Carbohydrate Changes during Cold Acclimation of Green‐Type Annual Bluegrass (Poa annua L.) Ecotypes

et al. 2001

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Winterkill is recurrently observed on annual bluegrass (Poa annua L.) golf greens in northern climates. Although annual bluegrass susceptibility to freezing temperatures has been pointed out as a major factor responsible for winter damages, little information exists on freezing tolerance and cold hardening of green‐type annual bluegrass. This study was conducted to assess freezing tolerance and carbohydrate changes occurring during cold acclimation of green‐type annual bluegrass ecotypes cold hardened under both environmentally controlled and simulated winter conditions in an unheated greenhouse. The 50% killing temperatures (LT50), levels of fructans, and mono and disaccharides were determined during cold acclimation in three annual bluegrass ecotypes originating from Western Pennsylvania (OK), Coastal Maryland (CO) and Central Québec (CR). The ecotypes differed significantly with regard to their freezing tolerance (LT50 ranking: OK < CO < CR) and maintained their relative ranking under both environmentally controlled and simulated‐natural winter conditions. Maximum freezing tolerance was observed after exposure to nonlethal subfreezing temperatures and annual bluegrass achieved high levels of freezing tolerance with LT50 of −31.2°C for OK, −24.6°C for CO, and −22.8°C for CR. High molecular weight fructans (DP>6) were the most abundant carbohydrates found in plants cold‐acclimated under low, nonfreezing temperature with levels up to 170 mg g−1 dry weight as compared with 60 to 70 mg g−1 dry weight in nonacclimated plants. Sucrose levels in crowns of annual bluegrass markedly increased at temperatures below freezing and maximum sucrose concentration coincided with maximum freezing tolerance of annual bluegrass. However, variations in fructan and sucrose levels were not related to differential freezing tolerance among the three annual bluegrass ecotypes tested.

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“…Long days (LDs) have been reported to favor accumulation of fructans (Hendry 1987), but this accumulation was probably caused by increased total irradiance in LDs (Solhaug 1991). Fructan synthesis under cold and water stress was compared in two Bromus species adapted to different climatic conditions (Puebla et al 1997). It was found that species adapted to a cold desert climate exhibited constitutive fructan synthesis, whereas species adapted to a warmer climate produced fructans only under cold stress.…”

Section: Introductionmentioning

confidence: 99%

Cold resistance in Antarctic angiosperms

Bravo¹,

Ulloa²,

Zúñiga³

et al. 2001

Physiologia Plantarum

126

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Deschampsia antarctica Desv. (Poaceae) and Colobanthus quitensis (Kunth) Bartl. (Cariophyllaceae) are the only two vascular plants that have colonized the Maritime Antarctic. The primary purpose of the present work was to determine cold resistance mechanisms in these two Antarctic plants. This was achieved by comparing thermal properties of leaves and the lethal freezing temperature to 50% of the tissue (LT50). The grass D. antarctica was able to tolerate freezing to a lower temperature than C. quitensis. The main freezing resistance mechanism for C. quitensis is supercooling. Thus, the grass is mainly a freezing‐tolerant species, while C. quitensis avoids freezing. D. antarctica cold acclimated; thus, reducing its LT50. C. quitensis showed little cold‐acclimation capacity. Because day length is highly variable in the Antarctic, the effect of day length on freezing tolerance, growth, various soluble carbohydrates, starch, and proline contents in leaves of D. antarctica growing in the laboratory under cold‐acclimation conditions was studied. During the cold‐acclimation treatment, the LT50 was lowered more effectively under long day (21/3 h light/dark) and medium day (16/8) light periods than under a short day period (8/16). The longer the day length treatment, the faster the growth rate for both acclimated and non‐acclimated plants. Similarly, the longer the day treatment during cold acclimation, the higher the sucrose content (up to 7‐fold with respect to non‐acclimated control values). Oligo and polyfructans accumulated significantly during cold acclimation only with the medium day length treatment. Oligofructans accounted for more than 80% of total fructans. The degrees of polymerization were mostly between 3 and 10. C. quitensis under cold acclimation accumulated a similar amount of sucrose than D. antarctica, but no fructans were detected. The suggestion that survival of Antarctic plants in the Antarctic could be at least partially explained by accumulation of these substances is discussed.

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Fructan metabolism in two species of Bromus subjected to chilling and water stress

Cited by 23 publications

References 27 publications

Importance of pre‐anthesis anther sink strength for maintenance of grain number during reproductive stage water stress in wheat

Importance of pre‐anthesis anther sink strength for maintenance of grain number during reproductive stage water stress in wheat

Freezing Tolerance and Carbohydrate Changes during Cold Acclimation of Green‐Type Annual Bluegrass (Poa annua L.) Ecotypes

Cold resistance in Antarctic angiosperms

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