Fructose and mannose metabolism in Aeromonas hydrophila: identification transport systems and catabolic pathways

Binet, Marie R. B.; Rager, Marie‐Noëlle; Bouvet, Odile

doi:10.1099/00221287-144-4-1113

Cited by 15 publications

(22 citation statements)

References 49 publications

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“…Mannans are converted to mannose by endo-and exo-acting mannanases (Centeno et al 2006). The mannose generated is phosphorylated by a transmembrane permease to mannose 6-phosphate (M6P) and then converted by a phosphomannose isomerase to fructose 6-phosphate (F6P) to flow into the Embden-Meyerhof-Parnas (EMP) pathway (Binet et al 1998). Previously, we showed that the gene for BfCE (locus tag BF0774) is located on an operon (locus tags from BF0771 to BF0774) in the B. fragilis NCTC 9343 genome (NC_003228) (Senoura et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Identification and distribution of cellobiose 2-epimerase genes by a PCR-based metagenomic approach

Wasaki

Taguchi

Senoura

et al. 2014

Appl Microbiol Biotechnol

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Cellobiose 2-epimerase (CE) catalyzes the reversible epimerization of cellobiose to 4-O-β-D-glucopyranosyl-D-mannose. By using a PCR-based metagenomic approach, 71 ce-like gene fragments were obtained from wide-ranging environmental samples such as sheep rumen, soils, sugar beet extracts, and anaerobic sewage sludge. The frequency of isolation of the fragments similar to known sequences varied depending on the nature of the samples used. The ce-like genes appeared to be widely distributed in environmental bacteria belonging to the phyla Bacteroidetes, Chloroflexi, Dictyoglomi, Firmicutes, Proteobacteria, Spirochaetes, and Verrucomicrobia. The phylogenetic analysis suggested that the cluster of CE and CE-like proteins was functionally and evolutionarily separated from that of N-acetyl-D-glucosamine 2-epimerase (AGE) and AGE-like proteins. Two ce-like genes containing full-length ORFs, designated md1 and md2, were obtained by PCR and expressed in Escherichia coli. The recombinant mD1 and mD2 exhibited low K m values and high catalytic efficiencies (k cat/K m) for mannobiose compared with cellobiose, suggesting that they should be named mannobiose 2-epimerase, which is involved in a new mannan catabolic pathway we proposed.

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Section: Discussionmentioning

confidence: 99%

Identification and distribution of cellobiose 2-epimerase genes by a PCR-based metagenomic approach

Wasaki

Taguchi

Senoura

et al. 2014

Appl Microbiol Biotechnol

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“…Addition of [1][2][3][4][5][6][7][8][9][10][11][12][13] C]glucose to whole cells of P. shigelloides resulted in the formation of C2-labeled ethanol, C3-labeled lactate, C2-labeled acetate and C2-labeled and C3-labeled succinate. This labeling pattern confirms that glucose is converted via the Embden±Meyerhof pathway [9].…”

Section: Discussionmentioning

confidence: 99%

“…Membrane and cytoplasmic fractions were prepared as described previously [5]. PTS activities were assayed by measuring the phosphoenolpyruvate-dependent phosphorylation of a-[ ).…”

Section: Complementation Assaysmentioning

confidence: 99%

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Plesiomonas shigelloides

2009

Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques

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The metabolism of mannose was examined in resting cells in vivo using 13 C-NMR and 31 P-NMR spectroscopy, in cell-free extracts in vitro using 31 P-NMR spectroscopy, and by enzyme assays. Plesiomonas shigelloides was shown to transport mannose by a phosphoenolpyruvate-dependent phosphotransferase system producing mannose 6-phosphate. However, a toxic effect was observed when P. shigelloides was grown in the presence of mannose.Investigation of mannose metabolism using in vivo 13C NMR showed mannose 6-phosphate accumulation without further metabolism. In contrast, glucose was quickly metabolized under the same conditions to lactate, ethanol, acetate and succinate. Extracts of P. shigelloides exhibited no mannose-6-phosphate isomerase activity whereas the key enzyme of the Embden±Meyerhof pathway (6-phosphofructokinase) was found. This result explains the mannose 6-phosphate accumulation observed in cells grown on mannose. The levels of phosphoenolpyruvate and P i were estimated by in vivo 31 P-NMR spectroscopy. The intracellular concentrations of phosphoenolpyruvate and P i were relatively constant in both starved cells and mannose-metabolizing cells. In glucose-metabolizing cells, the phosphoenolpyruvate concentration was lower, and about 80% of the P i was used during the first 10 min. It thus appears that the toxic effect of mannose on growth is not due to energy depletion but probably to a toxic effect of mannose 6-phosphate.Keywords: mannose; metabolism; microbial diversity; NMR; Plesiomonas shigelloides.The genus Plesiomonas appears to occupy a position between the families Enterobacteriaceae and Vibrionaceae in the gamma group of Proteobacteria [1]. Plesiomonas shigelloides is a facultatively anaerobic chemo-organotrophic Gram-negative bacterium. It has been implicated as the causative agent of gastroenteritis as well as of extraintestinal infections, primaly septicemia and meningitis [2]. To understand the ability of P. shigelloides to survive in its environment, it is essential to know the mechanisms by which nutrients are taken up and metabolized.In many Gram-negative bacteria, the specific phosphoenolpyruvate-dependent phosphotransferase system (PTS) mediates active transport and concomitant phosphorylation of sugars and hexitols [3,4]. The PTS uses phosphoenolpyruvate as the phosphoryl donor in a transfer chain that involves two general energy-coupling enzymes, Enzyme I and HPr, and the sugarspecific membrane-bound Enzyme II complex across the cell membrane.In order to study the metabolic diversity in Gram-negative bacteria, we have investigated sugar metabolism in P. shigelloides. In the present study, we show that mannose is transferred into the cells by a PTS producing mannose 6-phosphate. Curiously, when P. shigelloides was grown in nutrient broth supplemented with mannose, a lower growth yield than on nutrient broth was observed. Mannose and glucose catabolism were investigated in vivo, in resting cells using 13 C-NMR spectroscopy and in vitro, in cell-free extracts using 31 P-NMR spectroscopy an...

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“…It is generally established that mannose residues are phosphorylated by a transmembrane permease to mannose 6-phosphate and then converted to fructose 6-phosphate by an intracellular phosphomannose isomerase (Binet et al, 1998). This is surprising, considering the detailed information that is available on the hydrolysis of plant cell wall polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Galactomannan hydrolysis and mannose metabolism inCellvibrio mixtus

Centeno¹,

Guerreiro²,

Dias³

et al. 2006

FEMS Microbiology Letters

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Galactomannan hydrolysis results from the concerted action of microbial endo-mannanases, manosidases and alpha-galactosidases and is a mechanism of intrinsic biological importance. Here we report the identification of a gene cluster in the aerobic soil bacterium Cellvibrio mixtus encoding enzymes involved in the degradation of this polymeric substrate. The family 27 alpha-galactosidase, termed CmAga27A, preferentially hydrolyse galactose containing polysaccharides. In addition, we have characterized an enzyme with epimerase activity, which might be responsible for the conversion of mannose into glucose. The role of the identified enzymes in the hydrolysis of galactomannan by aerobic bacteria is discussed.

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Fructose and mannose metabolism in Aeromonas hydrophila: identification transport systems and catabolic pathways

Cited by 15 publications

References 49 publications

Identification and distribution of cellobiose 2-epimerase genes by a PCR-based metagenomic approach

Identification and distribution of cellobiose 2-epimerase genes by a PCR-based metagenomic approach

Plesiomonas shigelloides

Galactomannan hydrolysis and mannose metabolism inCellvibrio mixtus

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