Fructose-bisphosphate aldolase and pyruvate kinase, two novel immunogens in<i>Madurella mycetomatis</i>

Klerk, Nele de; Vogel, Corné P. de; Fahal, Ahmed H.; Belkum, Alex van; Sande, Wendy W. J. van de

doi:10.3109/13693786.2011.593005

Cited by 25 publications

(25 citation statements)

References 29 publications

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“…zooepidemicus through the blood-brain barrier. Finally, fructose-bisphosphate aldolase has been reported to be immunogenic in Candida albicans (Calcedo et al, 2012) and Madurella mycetomatis , in which it has been proposed as a vaccine candidate (de Klerk et al, 2012). …”

Section: Discussionmentioning

confidence: 99%

Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs

et al. 2017

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The spirochetes Brachyspira hyodysenteriae and B. pilosicoli are pig intestinal pathogens that are the causative agents of swine dysentery (SD) and porcine intestinal spirochaetosis (PIS), respectively. Although some inactivated bacterin and recombinant vaccines have been explored as prophylactic treatments against these species, no effective vaccine is yet available. Immunoproteomics approaches hold the potential for the identification of new, suitable candidates for subunit vaccines against SD and PIS. These strategies take into account the gene products actually expressed and present in the cells, and thus susceptible of being targets of immune recognition. In this context, we have analyzed the immunogenic pattern of two B. pilosicoli porcine isolates (the Spanish farm isolate OLA9 and the commercial P43/6/78 strain) and one B. hyodysenteriae isolate (the Spanish farm V1). The proteins from the Brachyspira lysates were fractionated by preparative isoelectric focusing, and the fractions were analyzed by Western blot with hyperimmune sera from challenged pigs. Of the 28 challenge-specific immunoreactive bands detected, 21 were identified as single proteins by MS, while the other 7 were shown to contain several major proteins. None of these proteins were detected in the control immunoreactive bands. The proteins identified included 11 from B. hyodysenteriae and 28 from the two B. pilosicoli strains. Eight proteins were common to the B. pilosicoli strains (i.e., elongation factor G, aspartyl-tRNA synthase, biotin lipoyl, TmpB outer membrane protein, flagellar protein FlaA, enolase, PEPCK, and VspD), and enolase and PEPCK were common to both species. Many of the identified proteins were flagellar proteins or predicted to be located on the cell surface and some of them had been previously described as antigenic or as bacterial virulence factors. Here we report on the identification and semiquantitative data of these immunoreactive proteins which constitute a unique antigen collection from these bacteria.

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Section: Discussionmentioning

confidence: 99%

Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs

et al. 2017

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“…The only serological tools which use recombinant antigens are the ELISA described by van de Sande and colleagues [48] and the Luminex described by de Klerk and associates [49]. The recombinant translationally controlled tumour protein (TCTP) was included in both assays.…”

Section: Serologymentioning

confidence: 99%

“…The recombinant translationally controlled tumour protein (TCTP) was included in both assays. The Luminex assay also included recombinant fructose-bisphosphate aldolase (FBA) and pyruvate kinase (PK) antigens [48], [49]. With these recombinant antigens it was noted that although patients gave higher antibody levels, healthy controls also produce antibodies against them, making it impractical to use these recombinant antigens as diagnostic tools.…”

Section: Serologymentioning

confidence: 99%

Merits and Pitfalls of Currently Used Diagnostic Tools in Mycetoma

et al. 2014

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Treatment of mycetoma depends on the causative organism and since many organisms, both actinomycetes (actinomycetoma) and fungi (eumycetoma), are capable of producing mycetoma, an accurate diagnosis is crucial. Currently, multiple diagnostic tools are used to determine the extent of infections and to identify the causative agents of mycetoma. These include various imaging, cytological, histopathological, serological, and culture techniques; phenotypic characterisation; and molecular diagnostics. In this review, we summarize these techniques and identify their merits and pitfalls in the identification of the causative agents of mycetoma and the extent of the disease. We also emphasize the fact that there is no ideal diagnostic tool available to identify the causative agents and that future research should focus on the development of new and reliable diagnostic tools.

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“…Although the precise function of TCTP in M. mycetomatis has not yet been defined, several findings suggest it may play a role in mycetoma development. For one, the protein is only expressed in the very young grain and not in aged grains, unlike the other two antigens known for M. mycetomatis: fructose bisphosphate aldolase (FBA) and pyruvate kinase (PK) [28]. Such stage-specific expression may be associated with invasiveness.…”

Section: Tctpmentioning

confidence: 99%

“…This latter test employs recombinant TCTP or in vitro synthesized TCTP-specific peptides, derived from the first M. mycetomatis-specific antigen so far identified. Further novel M. mycetomatis-specific antigens are currently being identified and serological testing may be significantly improved in the near future [28].…”

Section: Direct Identification In Tissue Samplesmentioning

confidence: 99%

Mycetoma Caused by Madurella mycetomatis: A Completely Neglected Medico-social Dilemma

Belkum

Fahal

Sande

2012

Advances in Experimental Medicine and Biology

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Mycetoma is a debilitating disease with a highly particular geographical distribution. The mycetoma belt circles the entire world just above the equator and defines the region with the highest prevalence and incidence. Although the disease is seen in Central America, India and all across Africa, Sudan seems to be the homeland of mycetoma. Mycetoma is an infectious disease caused either by bacteria (actinomycetoma) or true fungi (eumycetoma). In Sudan most cases are caused by the fungal species Madurella mycetomatis. The precise natural habitat of this fungus is still an enigma, but its DNA can easily be found in soil and plant samples in endemic areas. Although the entire human population in these areas are in regular contact with the fungus, most individuals are unaffected. Thus mycetoma is an ideal clinical and experimental model system for the study of host-pathogen interactions. Also, given its relative importance locally, improvements in clinical and laboratory diagnostics and knowledge of the epidemiology of the disease are badly needed. This chapter describes the current state of affairs in the field of eumycetoma caused by M. mycetomatis. The value of laboratory research on this disease and future perspective for control and prevention of the infection are discussed.

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Fructose-bisphosphate aldolase and pyruvate kinase, two novel immunogens inMadurella mycetomatis

Cited by 25 publications

References 29 publications

Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs

Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs

Merits and Pitfalls of Currently Used Diagnostic Tools in Mycetoma

Mycetoma Caused by Madurella mycetomatis: A Completely Neglected Medico-social Dilemma

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