Fructose-enabled killing of antibiotic-resistant Salmonella enteritidis by gentamicin: Insight from reprogramming metabolomics

Jiang, Ming; Li, Xia; Xie, Chunlin; Chen, Peng; Luo, Wei; Lin, C. Y.; Wang, Qiao; Shu, Dingming; Luo, Cheng-long; Qu, Hao; Ji, Jian

doi:10.1016/j.ijantimicag.2023.106907

Cited by 11 publications

(4 citation statements)

References 56 publications

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“…Membrane potential was determined by referring to the previous report . The procedure involved serial dilution of bacterial samples to approximately 10 6 cfu/mL, followed by the addition of 10 μL of 3 mM 3,3′-diethyloxacarbocyanine iodide ([DIOC2(3), Invitrogen]) to an Eppendorf tube containing 1 mL of the diluted bacterial solution.…”

Section: Materials and Methodsmentioning

confidence: 99%

“…Membrane potential was determined by referring to the previous report. 47 The procedure involved serial dilution of bacterial samples to approximately 10 6 cfu/mL, followed by the addition of 10 μL of 3 mM 3,3′-diethyloxacarbocyanine iodide ([DIOC2(3), Invitrogen]) to an Eppendorf tube containing 1 mL of the diluted bacterial solution. After incubation at 37 °C for 30 min, a FACSCalibur flow cytometer (Becton Dickinson, San Jose, CA) was employed for detection, with the excitation wavelengths for both green and red fluorescence set at 488 nm and the emission wavelengths set at 530 and 610 nm, respectively.…”

Section: Materials and Methodsmentioning

confidence: 99%

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Metabolomics Method in Understanding and Sensitizing Carbapenem-Resistant Acinetobacter baumannii to Meropenem

Li,

Feng,

Zhou

et al. 2023

ACS Infect. Dis.

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Carbapenem-resistant Acinetobacter baumannii (CRAB) strains are prevalent worldwide and represent a major threat to public health. However, treatment options for infections caused by CRAB are very limited as they are resistant to most of the commonly used antibiotics. Consequently, understanding the mechanisms underlying carbapenem resistance and restoring bacterial susceptibility to carbapenems hold immense importance. The present study used gas chromatography−mass spectrometry (GC− MS)-based metabolomics to investigate the metabolic mechanisms of antibiotic resistance in clinically isolated CRAB. Inactivation of the pyruvate cycle and purine metabolism is the most typical characteristic of CRAB. The CRAB exhibited a reduction in the activity of enzymes involved in the pyruvate cycle, proton motive force, and ATP levels. This decline in central carbon metabolism resulted in a decrease in the metabolic flux of the α-ketoglutarate-glutamate-glutamine pathway toward purine metabolism, ultimately leading to a decline in adenine nucleotide interconversion. Exogenous adenosine monophosphate (AMP) and adenosine triphosphate (ATP) enhance the killing efficacy of Meropenem against CRAB. The combination of ATP and Meropenem also has a synergistic effect on eliminating CRAB persisters and the biofilm, as well as protecting mice against peritonitis-sepsis. This study presents a novel therapeutic modality to treat infections caused by CRAB based on the metabolism reprogramming strategy.

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Section: Materials and Methodsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Metabolomics Method in Understanding and Sensitizing Carbapenem-Resistant Acinetobacter baumannii to Meropenem

Li,

Feng,

Zhou

et al. 2023

ACS Infect. Dis.

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“…It was found that this combination had a better anti-bacterial biofilm activity than GEN or quercetin alone. Jiang et al [ 3 ], discovered that GEN plus fructose exhibited a synergy antibiotic effect on Salmonella infections in chickens. Zhou et al [ 4 ], displayed that the exogenous supplementation of d -ribose was an adjuvant able to promote the effect of GEN on multidrug-resistant Salmonella .…”

Section: Introductionmentioning

confidence: 99%

Synergistic bactericidal combinations between gentamicin and chitosan capped ZnO nanoparticles: A promising strategy for repositioning this first-line antibiotic

Scolari,

Páez,

Granero

2024

Heliyon

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“…Reprogramming metabolomics is a new useful approach for controlling bacterial infection by using low doses of antibiotics or no antibiotics at all (5)(6)(7)(8)(9)(10)(11). This method requires the use of comparative metabolomics to identify crucial biomarkers, which are then used to induce metabolome-reprogramming for elevating bacterial sensitivity to antibiotics or improving host protection against infection (12)(13)(14)(15)(16). This leads to subsequent host protection against infections caused by antibiotic-sensitive orresistant Vibrio alginolyticus and Edwardsiella tarda (17-21).…”

Section: Introductionmentioning

confidence: 99%

Aspartate metabolic flux promotes nitric oxide to eliminate both antibiotic-sensitive and -resistant Edwardsiella tarda in zebrafish

Xiang,

Li,

2023

Front. Immunol.

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IntroductionMetabolic reprogramming potentiates host protection against antibiotic-sensitive or -resistant bacteria. However, it remains unclear whether a single reprogramming metabolite is effective enough to combat both antibiotic-sensitive and -resistant bacteria. This knowledge is key for implementing an antibiotic-free approach.MethodsThe reprogramming metabolome approach was adopted to characterize the metabolic state of zebrafish infected with tetracycline-sensitive and -resistant Edwardsiella tarda and to identify overlapping depressed metabolite in dying zebrafish as a reprogramming metabolite.ResultsAspartate was identify overlapping depressed metabolite in dying zebrafish as a reprogramming metabolite. Exogenous aspartate protects zebrafish against infection caused by tetracycline-sensitive and -resistant E. tarda. Mechanistically, exogenous aspartate promotes nitric oxide (NO) biosynthesis. NO is a well-documented factor of promoting innate immunity against bacteria, but whether it can play a role in eliminating both tetracycline-sensitive and -resistant E. tarda is unknown. Thus, in this study, aspartate was replaced with sodium nitroprusside to provide NO, which led to similar aspartate-induced protection against tetracycline-sensitive and -resistant E. tarda.DiscussionThese findings support the conclusion that aspartate plays an important protective role through NO against both types of E. tarda. Importantly, we found that tetracycline-sensitive and -resistant E. tarda are sensitive to NO. Therefore, aspartate is an effective reprogramming metabolite that allows implementation of an antibiotic-free approach against bacterial pathogens.

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Fructose-enabled killing of antibiotic-resistant Salmonella enteritidis by gentamicin: Insight from reprogramming metabolomics

Cited by 11 publications

References 56 publications

Metabolomics Method in Understanding and Sensitizing Carbapenem-Resistant Acinetobacter baumannii to Meropenem

Metabolomics Method in Understanding and Sensitizing Carbapenem-Resistant Acinetobacter baumannii to Meropenem

Synergistic bactericidal combinations between gentamicin and chitosan capped ZnO nanoparticles: A promising strategy for repositioning this first-line antibiotic

Aspartate metabolic flux promotes nitric oxide to eliminate both antibiotic-sensitive and -resistant Edwardsiella tarda in zebrafish

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