Fructose-fed streptozotocin-injected rat: an alternative model for type 2 diabetes

Abstract

The main objective of the study was to develop an alternative non-genetic rat model for type 2 diabetes (T2D). Six-week-old male Sprague-Dawley rats (190.56 ± 23.60 g) were randomly divided into six groups, namely: Normal Control (NC), Diabetic Control (DBC), Fructose-10 (FR10), Fructose-20 (FR20), Fructose-30 (FR30) and Fructose-40 (FR40) and were fed a normal rat pellet diet ad libitum for 2 weeks. During this period, the two control groups received normal drinking water whilst the fructose groups received 10, 20, 30 and 40% fructose in drinking water ad libitum, respectively. After two weeks of dietary manipulation, all groups except the NC group received a single injection (i.p.) of streptozotocin (STZ) (40 mg/kg b.w.) dissolved in citrate buffer (pH 4.4). The NC group received only a vehicle buffer injection (i.p.). One week after the STZ injection, animals with non-fasting blood glucose levels > 300 mg/dl were considered as diabetic. Three weeks after the STZ injection, the animals in FR20, FR30 and FR40 groups were eliminated from the study due to the severity of diabetes and the FR10 group was selected for the remainder of the 11 weeks experimental period. The significantly (p < 0.05) higher fluid intake, blood glucose, serum lipids, liver glycogen, liver function enzymes and insulin resistance (HOMA-IR) and significantly (p < 0.05) lower body weight, oral glucose tolerance, number of pancreatic β-cells and pancreatic β-cell functions (HOMA-β) of FR10 group demonstrate that the 10% fructose-fed followed by 40 mg/kg of BWSTZ injected rat can be a new and alternative model for T2D.

Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

“…Previously numerous phytochemical constituents were reported to improve glycemic control by stimulating remnant β-cells, increasing the mass of β-cells and/or enhancing the insulin action [28, 29]. Additionally fasting or postprandial hyperglycemia is a common pathogenesis of diabetes mellitus which is induced by partial pancreatic β-cell destruction and/or insulin resistance [30]. The quantitative assessment of insulin resistance (HOMA-IR) and insulin production/β-cell function (HOMA-β) are key factors to assess control of diabetes and glucose homeostasis [31].…”

Amelioration of Early Markers of Diabetic Nephropathy by Linagliptin in Fructose-Streptozotocin-Induced Type 2 Diabetic Rats

“…All animals were fasted overnight and each of the Fr-fed animal was injected with a low single-dose STZ (40 mg/kg; i.p.) [32]. Fr-STZ rats were given glucose (10%) solution during the first 24 h of diabetes to avoid acute hypoglycemia.…”

Use of Polysaccharide extracted from Tremella fuciformis Berk for control diabetes induced in rats.

“…On the other model involving diet, the time to evaluate the animals was higher being able to use the polysaccharide at the maximum of 60 days, similar data was described by Wilson and Islam (2012) in animal models, however the monetary cost has been being too high. Table 2 displays the results of the biochemical tests at the time of euthanasia.…”