Fructus <i>Ligustrum lucidi</i> inhibits inflammatory mediator release through inhibition of nuclear factor-κB in mouse peritoneal macrophages

An, Hyo‐Jin; Jeong, Hyun‐Ja; Um, Jae‐Young; Park, Yun-Jum; Park, Raekil; Kim, Eun-Cheol; Na, Ho-Jeong; Shin, Tae‐Yong; Kim, Hyung-Min; Hong, Seung‐Heon

doi:10.1211/jpp.59.9.0013

Cited by 14 publications

(14 citation statements)

References 22 publications

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“…In addition, FLL treatment upregulates the Bcl-2 expression and inhibits the release of Cyto-C into the cytoplasm in the tibias of OVX rats. Similarly, FLL is demonstrated to possess the ability of inhibiting NF-κB activation and decreasing IκBα phosphorylation in mouse peritoneal macrophages (An et al, 2007). Further, the resultant estrogen deficiency by ovariectomy stimulates mitochondrial malfunctions for increasing ROS production (Yazgan et al, 2016) and Cyto-C expression (Li et al, 2014) as well as decreasing Bcl-2 expression (Lin et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Effect of Fructus Ligustri Lucidi Aqueous Extract in Ovariectomized Rats Is Mediated through Nox4-ROS-NF-κB Pathway

Wang

Guo

et al. 2017

Front. Pharmacol.

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Purpose: This study is designed to explore whether Fructus ligustri lucidi (FLL) exhibits antioxidant effect in ovariectomized (OVX) rats, and to identify the signaling pathway involved in this process.Methods: OVX rats were treated with FLL aqueous extract (3.5 g/kg) for 12 weeks. Serum, uteri, and tibias were harvested from the rats and the levels of total antioxidant capacity (TAC), nitric oxide (NO), malondialdehyde (MDA), 8-hydroxy-desoxyguanosine (8-OHdG), and superoxide dismutase (SOD) were determined. Changes in the levels of NF-κB-p65, phosphorylation of NF-κB-p65 (NF-κB-pp65), NF-κB inhibitor alpha (IκBα), phosphorylation of IκBα (p-IκBα), and NADPH oxidase 4 (Nox4) in uteri and tibias were determined by western blot, immunofluorescent and immunohistochemical analysis, respectively. In addition, the expression of cytochrome C (Cyto-C) and B-cell lymphoma-2 (Bcl-2) were determined in the tibias of rats. Histopathological changes in the bones were evaluated by hematoxylin-eosin staining. Bone mineral density (BMD) was determined in rat femurs by dual X-ray absorptiometry.Results: Treatment of OVX rats with FLL aqueous extract improved redox homeostasis by increasing the levels of TAC and NO as well as decreasing the levels of MDA and 8-OHdG in serum, tibias, and uteri. Further, FLL extract also downregulated the expression of Nox4, NF-κB-p65, NF-κB-pp65, and p-IκBα in the uteri and tibias. Furthermore, administration of FLL–OVX rats increased Bcl-2 expression and prevented cytoplasmic release of mitochondrial Cyto-C in the tibias. In addition, FLL treatment also improved bone microstructure and increased cortical bone thickness as well as increased BMD values in the femurs of OVX rats.Conclusions: FLL treatment may suppress oxidative stress response in OVX rats via regulating the Nox4/ROS/NF-κB signaling pathway. These results suggest the potential of using FLL as a natural antioxidant agent in preventing the development of osteoporosis.

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Section: Discussionmentioning

confidence: 99%

Antioxidant Effect of Fructus Ligustri Lucidi Aqueous Extract in Ovariectomized Rats Is Mediated through Nox4-ROS-NF-κB Pathway

Wang

Guo

et al. 2017

Front. Pharmacol.

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“…We found that LM leaf extract at 0.25 and 1 g/kg decreased the percentage of abdominal Evan's blue extravasation caused by LPS. Therefore, we suggested that LM leaf extract similar to the reported of L. lucidum (Wang et al, 2002;An et al, 2007) inhibits inflammatory response caused by bacterial toxin LPS. This effect may be due to the blockade of inflammatory mediators' release and prostaglandin synthesis.…”

Section: Analgesic and Anti-inflammation Of Ligustrum Morrisonensementioning

confidence: 69%

Analgesic and Anti-Inflammatory Property of the Methanol Extract from Ligustrum morrisonense Leaves in Rodents

Lin

et al. 2011

Am. J. Chin. Med.

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Ligustrum morrisonense Kaneh and Sasaki (abbreviated as LM), an endemic Ligustrum plant in Taiwan, is similar to Ligustrum lucidum, which is usually used for curing hepatic and inflammatory disorders. The aim of this study was to evaluate the analgesic and anti-inflammatory properties of LM by chemical-induced algesia and carrageenan-induced inflammation in rodents. Its triterpenoid contents were measured by using high performance liquid chromatography-photodiode array detector. LM leaf extracts effectively inhibited writhing responses induced by 1% acetic acid and biphasic-licking responses caused by 1% formalin. LM leaf extract also reduced the edema induced by 1% carrageenan. Furthermore, LM leaf extract reduced the abdominal Evan's blue extravasations caused by lipopolysaccharide (LPS), serotonin, histamine and bradykinin. LM leaf extract has higher contents of amyrin and lupeol among six assayed triterpenoid compounds. In conclusion, LM is a potential analgesic and anti-inflammatory Ligustrum plant, and its anti-inflammatory effects are partially related to decreasing microvascular permeability via inflammatory mediators and inhibiting cyclooxygenase-2 activity.

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“…An et al (15) found that FLL treatment reduced inflammation via inhibition of nuclear factor-κB in mouse peritoneal C D …”

Section: Effects Of Fll On Mechanical Allodynia and Thermal Hyperalgementioning

confidence: 99%

Comparative analysis of the influence of Fructus Ligustri Lucidi on a rat lumbar disc herniation model

Han

Liang

Han

et al. 2012

Molecular Medicine Reports

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Abstract. Lumbar disc herniation (LDH) is a term used for a group of conditions, including back pain, femoral nerve pain and sciatica. Currently available treatments and surgical options are insufficient for patients with LDH. Fructus Ligustri Lucidi (FLL) is a herb that is used for treating age-associated diseases. The results of the present study suggested that FLL may be used for treatment of patients with LDH. In the present study, matrix metalloproteinase-1, -3, -8 and -9 (MMP-1, -3, -8 and -9) protein and mRNA expression downregulation was observed in patients with LDH according to western blotting and reverse transcription-quantitative polymerase chain reaction. By contrast, upregulation of interleukin-2 (IL-2), IL-6, IL-8 and tumor necrosis factor-α (TNF-α) expression was observed in patients with LDH, according to an enzyme-linked immunosorbent assay. Mechanical allodynia was observed in rats with LDH not treated with FLL; however, not in FLL-treated rats. IL-2, IL-6, IL-8 and TNF-α expression levels in the serum from untreated rats were significantly higher than that of the FLL-treated rat models. Protein expression levels of MMPs in FLL-treated rats were lower than those in untreated rats. However, the mechanisms underlying the association between FLL and protein expression levels require further investigation.

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Fructus Ligustrum lucidi inhibits inflammatory mediator release through inhibition of nuclear factor-κB in mouse peritoneal macrophages

Cited by 14 publications

References 22 publications

Antioxidant Effect of Fructus Ligustri Lucidi Aqueous Extract in Ovariectomized Rats Is Mediated through Nox4-ROS-NF-κB Pathway

Antioxidant Effect of Fructus Ligustri Lucidi Aqueous Extract in Ovariectomized Rats Is Mediated through Nox4-ROS-NF-κB Pathway

Analgesic and Anti-Inflammatory Property of the Methanol Extract from Ligustrum morrisonense Leaves in Rodents

Comparative analysis of the influence of Fructus Ligustri Lucidi on a rat lumbar disc herniation model

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