Fruit Wastes as Substrate For the Production of Amylase by Aspergillus niger

Abstract

Waste generation demands that measures must be put in place in order to avert their detrimental effect to the environment. Bioconversion of agricultural waste to useful products like enzyme is a welcome development. Amylase production by Aspergillus niger via submerged fermentation of fruit wastes such as pineapple, orange, banana, cucumber and watermelon was investigated. Biomass of A. niger, amylase produced and pH of the fermenting fruit waste media were determined using standard techniques during submerged fermentation. From the data obtained Banana waste medium produced the highest yield of A. niger amylase (259.00 ± 1.23 U/mL) compared to other waste media at 4 d of fermentation. Amylase yield from Banana waste medium was significantly higher compared with the other fruit wastes (p < 0.05). Among the various supplemented nitrogen sources in the Banana waste medium, ammonium nitrate gave the highest amylase yield of 881.00 ± 16.97 U/mL while the least (549.5 ± 20.51 U/mL) was from potassium nitrate medium. Thus, the study revealed that A. niger can be used for amylase production from fruit waste and optimal yield can be enhanced by supplementing the medium with ammonium nitrate.

The influence of nitrogen supplementation on lipase production by Aspergillus niger using palm oil mill effluent

“…nitrogen source gave the highest yield of biomass and lipase by A. niger in submerged fermentation (George-Okafor et al, 2013;Mishra et al, 2016;Oshoma et al, 2017). Thus, confirming our findings that medium supplemented with (NH ) SO enhanced fungal growth which in turn,…”

“…Nitrogen is the main building block of proteins (enzyme) and is one of the main constituents of living organisms. Supplementation of nitrogen is used to optimize the nitrogen requirement of most microbial species especially fungi (Oshoma et al, 2017). The ability of the various nitrogen sources mostly ammonium sulphate to support POME as a substrate to increase fungal growth confirmed the substrate to be a suitable medium for fungal growth and metabolite production (Costa et al, 2017).…”

“…The POME medium that gave the highest yield of lipase was supplemented with different nitrogen sources. The modified method of Oshoma et al (2017) prepared in triplicates, were autoclaved at 121 C for 15 min. The flasks containing the various nitrogen supplemented media were inoculated with 500 µl spores' suspension of A. niger, while the control medium was without nitrogen source.…”

The Effect of Copper (II) ion on the Production of Citric Acid by Aspergillus niger using Corncobs

“…The fungal isolate was identified based on cultural and microscopy characterization following standard methods and maintained on potato dextrose agar (PDA) slant and stored at 4 o C. Inoculum was prepared from a subculture of Aspergillus niger on PDA plates and incubated for 5 d. After the inoculation period, a solution of 1 % tween 80 was prepared and 10 ml of the solution was poured each time onto the cultured agar plate and the spores was gently scrapped using sterile glass rod. The number of spores was counted using haemocytometer and inoculum size of 10 6 spores/ml was used to inoculate all the media [18,19].…”

“…o C for 15 mins, cooled and inoculated with 1ml ( 106 spores/ml) from the homogenate Aspergillus niger culture aseptically transferred into each medium [19]. Parameters analyzed at every 2 d interval were citric acid concentration, fungal biomass and pH.…”