Fruits as the vehicle of drug resistant pathogenic yeasts

Lo, Hsiu‐Jung; Tsai, Sheng-Hua; Chu, Wen-Li; Chen, Yin-Zhi; Zhou, Zi-Li; Chen, Hong-Fu; Lee, Ching Fu; Yang, Yun-Liang

doi:10.1016/j.jinf.2017.06.005

Cited by 38 publications

(27 citation statements)

References 29 publications

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“…Two environmental surveys have been conducted in China. In one, DST225 strains were isolated from fruits and patients in different hospitals and showed cross-resistance to fluconazole and triadimenol (an azole fungicide) (Lo et al, 2017). The other was an analysis of soil samples.…”

Section: Discussionmentioning

confidence: 99%

“…However, fluconazole-nonsusceptible (FNS) C. tropicalis is relatively rare in Europe and America (Corzo-Leon et al, 2014;Scordino et al, 2018;Beyer et al, 2019). Some studies support the link between FNS C. tropicalis infections and prior antifungal exposure (Choi et al, 2016;Chen et al, 2019), whereas others suggest that FNS C. tropicalis may come from the environment (Lo et al, 2017;Wang et al, 2018). Unfortunately, the evolution and geographical distribution of these drugresistant genotypes remain unclear and require further and more extensive research.…”

Section: Introductionmentioning

confidence: 99%

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Multilocus Sequence Typing Reveals Clonality of Fluconazole-Nonsusceptible Candida tropicalis: A Study From Wuhan to the Global

Wang

Tang

et al. 2020

Front. Microbiol.

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Candida tropicalis is a globally distributed human pathogenic yeast, and its increasing resistance to azoles makes clinical treatment difficult. In this study, we investigated the clinical features, azole resistance and genetic relatedness of 87 C. tropicalis isolates from central China and combined with the global database to explore the relationship between genetic information and fluconazole susceptibility. Of the 55 diploid sequence types (DSTs) identified by multilocus sequence typing (MLST), 27 DSTs were new to the C. tropicalis MLST database. Fluconazole-nonsusceptible (FNS) isolates were genetically closely related. goeBURST analysis showed that DST225, DST376, DST506, and DST546 formed a distinct and unique FNS clonal complex (CC) in Wuhan. The local FNS CC belongs to the large FNS CC (CC2) in China, in which the putative founder DST225 has been reported from the environment. The three most prevalent types (DST506, DST525, and DST546) in Wuhan had high minimum inhibitory concentrations (MICs) for antifungal azoles, and the six possible nosocomial transmissions we captured were all FNS strains, most of which were from CC2. Unique FNS CCs have been found in Singapore (CC8) and India (CC17) and are close to China’s CC2 in the minimum spanning tree. There were no FNS CCs outside Asia. This study is the first to reveal a significant correlation between genetic information and fluconazole susceptibility worldwide and to trace geographical locations, which is of great value for molecular epidemiological surveillance and azole-resistance study of C. tropicalis globally.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multilocus Sequence Typing Reveals Clonality of Fluconazole-Nonsusceptible Candida tropicalis: A Study From Wuhan to the Global

Wang

Tang

et al. 2020

Front. Microbiol.

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“…In previous studies, DST225 can be isolated from fruits and soil, and resistant to fluconazole, supporting the transfer of azole‐resistant C . tropicalis from agriculture to human hosts (Lo et al, 2017; Yang et al, 2012). The shared DSTs in Wuhan, Taiwan, and Shanghai may be related to human activities and the transportation of agricultural products .…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of Candida tropicalis isolated from urogenital tract infections

Wang

Tang

et al. 2020

MicrobiologyOpen

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Candida spp. are important components of the microflora of human skin, oral and vaginal mucosa, and gastrointestinal tract. They are important opportunistic pathogens that can cause superficial and invasive infections in human hosts (Falagas et al., 2010). Over the past decade, infections caused by Candida have risen significantly and become an important cause of nosocomial infections, leading to serious public health problems (Gajdács

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“…Of the 12 fluconazole resistant isolates obtained, four belonged to DST225, including two pan-azole resistant isolates (Ct07R and Ct09R). Interestingly, human and fruit samples have recently been found to contain DST225 C. tropicalis in Taiwan and these isolates have been shown to be less susceptible to azole drugs [ 23 ]. This suggests that C. tropicalis fluconazole resistance is related to specific genotypes, such as DST225.…”

Section: Discussionmentioning

confidence: 99%

MDR1 overexpression combined with ERG11 mutations induce high-level fluconazole resistance in Candida tropicalis clinical isolates

et al. 2018

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BackgroundMarked increases in fluconazole resistance in Candida tropicalis have been recently reported. In this study, the molecular mechanisms behind fluconazole resistance were investigated.MethodsTwenty-two C. tropicalis clinical isolates, including 12 fluconazole-resistant isolates and 10 fluconazole-susceptible isolates, were collected from a tertiary care teaching hospital in Beijing between 2013 and 2017. Antifungal susceptibility testing, multilocus sequence typing, ERG11 amplification and sequencing, quantitative real-time reverse transcription-polymerase chain reaction (ERG11, UPC2, MDR1, and CDR1), and clinical data collection were performed for all C. tropicalis isolates.ResultsMultilocus sequence typing revealed that the 10 fluconazole-susceptible isolates and 12 fluconazole-resistant isolates were divided into nine and seven diploid sequence types, respectively. Of the 12 patients with fluconazole-resistant isolates, six had been previously exposed to azole and four had a fatal outcome. Y132F and S154F amino acid substitutions in Erg11p were found in all fluconazole-resistant isolates except one. MDR1 gene overexpression was identified in fluconazole-resistant isolates. In particular, seven high-level fluconazole resistant isolates (minimum inhibitory concentration ≥ 128 mg/L) and three pan-azole resistant isolates were identified. CDR1, ERG11, and UPC2 gene expression levels in fluconazole-resistant isolates were not significantly different from the control isolates (P = 0.262, P = 0.598, P = 0.114, respectively).ConclusionsThis study provides evidence that the combination of MDR1 gene overexpression and ERG11 missense mutations is responsible for high-level fluconazole resistance and pan-azole resistance in C. tropicalis clinical isolates. To the best of our knowledge, this is the first study investigating the relationship between MDR1 gene overexpression and increased fluconazole resistance.

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Fruits as the vehicle of drug resistant pathogenic yeasts

Cited by 38 publications

References 29 publications

Multilocus Sequence Typing Reveals Clonality of Fluconazole-Nonsusceptible Candida tropicalis: A Study From Wuhan to the Global

Multilocus Sequence Typing Reveals Clonality of Fluconazole-Nonsusceptible Candida tropicalis: A Study From Wuhan to the Global

Molecular epidemiology of Candida tropicalis isolated from urogenital tract infections

MDR1 overexpression combined with ERG11 mutations induce high-level fluconazole resistance in Candida tropicalis clinical isolates

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