Fruits from ripening impaired, chlorophyll degraded and jasmonate insensitive tomato mutants have altered tocopherol content and composition

Almeida, Juliana; Ramón, Adriana Noemí; Molineri, Virginia Noel; Sestari, Ivan; Lira, Bruno Silvestre; Carrari, Fernando; Peres, Lázaro Eustáquio Pereira; Rossi, Magdalena

doi:10.1016/j.phytochem.2014.11.007

Cited by 40 publications

(74 citation statements)

References 78 publications

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“…Fruits at the MG stage were detached from the plants and let to ripen under constant white light or darkness (Figure a). As previously reported (Almeida et al, ), the tocopherol levels increased during ripening in WT fruits. However, the accumulation was higher when the fruits were maintained under constant white light, indicating that this stimulus promotes tocopherol biosynthesis.…”

Section: Resultssupporting

confidence: 87%

“…A network analysis based on a dedicated transcriptional and metabolite data showed that tocopherol biosynthesis is transcriptionally regulated, pinpointed the link between Chl and tocopherol metabolism, and revealed that the supply of the prenyl donor is limiting for VTE accumulation at later stages of fruit development (Quadrana et al, ). These hypotheses were further confirmed by metabolic and transcript profiling of fruits from Chl breakdown and ripening‐impaired mutants (Almeida et al, ). During green stages, tomato GGDR (SlGGDR) produces de novo PDP for Chl biosynthesis and Chl turnover feeds tocopherol pathway via phytol recycling (Almeida et al, ).…”

Section: Introductionmentioning

confidence: 74%

“…Tocopherol biosynthesis has been well described during tomato fruit development and ripening (Almeida et al, , ). A network analysis based on a dedicated transcriptional and metabolite data showed that tocopherol biosynthesis is transcriptionally regulated, pinpointed the link between Chl and tocopherol metabolism, and revealed that the supply of the prenyl donor is limiting for VTE accumulation at later stages of fruit development (Quadrana et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…The MEP intermediate geranylgeranyl diphosphate (GGDP) is the precursor for the production of carotenoids and PDP, which is further used for both, Chl and tocopherol biosynthesis ( Figure 1). Conversion of GGDP into PDP is catalysed by the enzyme GERANYLGERANYL DIPHOS-PHATE REDUCTASE (GGDR; Almeida et al, 2015;DellaPenna & Pogson, 2006;Quadrana et al, 2013). Additionally, besides the de novo synthesis from GGDP, PDP can be produced from the recycling of Chl degradation-derived phytol catalysed by PHYTOL KINASE (VTE5) and PHYTYL PHOSPHATE KINASE (VTE6; Ischebeck, Zbierzak, Kanwischer, & Dörmann, 2006;vom Dorp et al, 2015;Figure 1).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, besides the de novo synthesis from GGDP, PDP can be produced from the recycling of Chl degradation-derived phytol catalysed by PHYTOL KINASE (VTE5) and PHYTYL PHOSPHATE KINASE (VTE6; Ischebeck, Zbierzak, Kanwischer, & Dörmann, 2006;vom Dorp et al, 2015;Figure 1). Tocopherol biosynthesis has been well described during tomato fruit development and ripening (Almeida et al, 2011(Almeida et al, , 2015. A network analysis based on a dedicated transcriptional and metabolite data showed that tocopherol biosynthesis is transcriptionally regulated, pinpointed the link between Chl and tocopherol metabolism, and revealed that the supply of the prenyl donor is limiting for VTE accumulation at later stages of fruit development (Quadrana et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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PHYTOCHROME‐INTERACTING FACTOR 3 mediates light‐dependent induction of tocopherol biosynthesis during tomato fruit ripening

Gramegna

Rosado

Carranza

et al. 2018

Plant Cell & Environment

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Tocopherols are important antioxidants exclusively produced in plastids that protect the photosynthetic apparatus from oxidative stress. These compounds with vitamin E activity are also essential dietary nutrients for humans. Although the tocopherol biosynthetic pathway has been elucidated, the mechanisms that regulate tocopherol production and accumulation remain elusive. Here, we investigated the regulatory mechanism underlying tocopherol biosynthesis during ripening in tomato fruits, which are an important source of vitamin E. Our results show that ripening under light conditions increases tocopherol fruit content in a phytochrome‐dependent manner by the transcriptional regulation of biosynthetic genes. Moreover, we show that light‐controlled expression of the GERANYLGERANYL DIPHOSPHATE REDUCTASE (SlGGDR) gene, responsible for the synthesis of the central tocopherol precursor phytyl diphosphate, is mediated by PHYTOCHROME‐INTERACTING FACTOR 3 (SlPIF3). In the absence of light, SlPIF3 physically interacts with the promoter of SlGGDR, down‐regulating its expression. By contrast, light activation of phytochromes prevents the interaction between SlPIF3 and the SlGGDR promoter, leading to transcriptional derepression and higher availability of the PDP precursor for tocopherol biosynthesis. The unraveled mechanism provides a new strategy to manipulate fruit metabolism towards improving tomato nutritional quality.

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Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 74%