FSCN1 Promotes Glycolysis and Epithelial-Mesenchymal Transition in Prostate Cancer through a YAP/TAZ Signaling Pathway

Abstract

Objective. The aim of the study is to investigate the role and possible mechanism of fascin-1 (FSCN1) in the invasion, migration, glycolysis, and epithelial-mesenchymal transition (EMT) of prostate cancer. Methods. Real-time quantitative polymerase chain reaction (qRT-PCR) was utilized to determine the mRNA expression level of FSCN1 in prostate cancer tissues and prostate cancer cells PC-3 and DU145. The transwell and the scratch test were applied to detect the invasion and migration abilities of cells, respectively. A metabolic assay was used for measuring the glucose consumption, lactate production, and the extracellular acidification rate (ECAR) in cells; western blot was used for checking FSCN1, EMT, and yes-associated protein/transcriptional co-activators with the PDZ-binding motif (YAP/TAZ) signaling pathway-related protein expression level in cells or tissues. Results. FSCN1 was significantly highly expressed in prostate cancer tissues and cells. On the one hand, interference with the expression of FSCN1 could inhibit the invasion, migration, EMT, and glycolysis of prostate cancer cells. On the other hand, overexpression of FSCN1 promoted the invasion, migration, EMT, and glycolysis of prostate cancer cells. Besides, further mechanistic studies revealed that FSCN1 could activate the YAP/TAZ signaling pathway in prostate cancer cells. Conclusion. FSCN1 promotes invasion, migration, EMT, and glycolysis in prostate cancer cells by activating the YAP/TAZ signaling pathway. FSCN1 may be used as a biomarker for the diagnosis or treatment in prostate cancer.

The Role of Fascin-1 in Human Urologic Cancers: A Promising Biomarker or Therapeutic Target?

“…97,98 Limited and hormone-refractory PCas show a significant upregulation of fascin-1 expression than that in benign prostate tissue. 6,68,69,99 In addition, a follow-up of patients undergoing radical prostatectomy has shown high fascin-1 expression to be related to an increased rate of PSA recurrence. 68 High fascin-1 expression is related to Gleason score and bone metastases, 69 thus confirming its role in PCa progression.…”

“…70 Thus, targeting fascin-1 may be effective in preventing or treating metastatic PCa. 68,99,100 Octavian and Daniel et al investigated serum fascin-1 levels. 67,70 They showed that although serum fascin-1 levels differ in patients with and without PCa, the differences are not statistically significant.…”

“…The results of cellular assays suggest that silencing the fascin-1 gene reduces tumor cell motility and invasiveness and increases cell adhesion. 68 , 99 , 100 In vivo experiments have shown that reducing fascin-1 expression in mice significantly inhibits tumor development and suppresses lymph node metastasis. 70 Thus, targeting fascin-1 may be effective in preventing or treating metastatic PCa.…”

“… 70 Thus, targeting fascin-1 may be effective in preventing or treating metastatic PCa. 68 , 99 , 100 …”

LncRNA PTOV1-AS2 Promotes Colon Cancer Progression through the miR-145-5p/FSCN1 Axis

“…As a muscle-binding protein, FSCN1 plays a key role in cell migration, motility, adhesion, and cell-cell interactions. Furthermore, some studies have found that FSCN1 is aberrantly expressed in various cancers and is closely associated with the malignant behaviour of tumours [ 18 ].…”