FT-IR spectroscopic characteristics of differently cultivated Bacillus subtilis

Abstract

Filip Z., Hermann S., Demnerová K. (2008): FT-IR spectroscopic characteristics of differently cultivated Escherichia coli. Czech J. Food Sci., 26: 458-463. FT-IR spectra were recorded of Escherichia coli cell mass with the aim of obtaining spectral traits possibly useful in a rapid detection and characterisation of this indicator bacterium. A well differentiated spectrum was obtained from the cell mass harvested in a stationary phase of growth, e.g., after 24 h, from a minimum nutrient broth. The cell mass, harvested either earlier or grown in nutrient solutions which contained an enhanced carbon or nitrogen concentrations delivered somewhat different IR spectra, apparently due to a higher content of nucleic acid components as related to other structural constituents of bacterial cells. Consequently, the FT-IR spectra of E. coli, although rather rapidly to collect, seem only capable of delivering useful and reproducible information if the cell mass is obtained under standardised cultural conditions.

Application of ultra-sonication, acid precipitation and membrane filtration for co-recovery of protein and humic acid from sewage sludge

“…6, the spectral regions at 1662 cm -1 , 1541 cm -1 and 1210 (1240) cm -1 of the recovered proteins assigned to the existence of Amide I, Amide II and Amide III related materials, resemble the previous study of Filip et al [31], in which the content of Amide III was higher than that of Amide I and Amide II. The band located around 3200 cm -1 was generally attributed to OH groups, and bands at 2950-2850 cm -1 and 1400 cm -1 assigned to aliphatic C -H, C -H 2 and C -H 3 stretching, indicating the presence of the lipoprotein related long alkyl chains.…”

Synergistic Enhancement in Catalytic Performance of Superparamagnetic Fe3O4@Bacilus subtilis as Recyclable Fenton-Like Catalyst

“…Bacillus subtilis is a kind of genus Bacillus and can be easily found in water, soil, air, and decomposing plant matter [13][14][15]. Thus, owing to its distinguished physicochemical/biological properties, B. subtilis has been selected as an ideal candidate for the synthesis of composite materials for organic pollutants removal [13][14][15][16].…”

FT-IR spectroscopic characteristics of differently cultivated Escherichia coli

“…In our early investigations, we obtained well differentiated IR spectra from a complex soil microbial biomass (Filip 1978). Later on, we used the Fourier Transform-IR spectroscopy (FT-IR) for the characterisation of the cell mass of Pseudomonas spp., and Bacillus subtilis (Filip & Hermann 2001;Filip et al 2004).…”