FT-IR spectroscopic study of amyloid protein formation and aortic valve calcification

Mamarelis, Ioannis; Koutoulakis, Emmanouil; Kotoulas, Christophoros; Dritsa, V.; Mamareli, Vasiliki; Anastassopoulou, J.

doi:10.1016/j.hjc.2016.09.011

Cited by 7 publications

(13 citation statements)

References 6 publications

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“…The next high intensity band at 1650 cm -1 is assigned to the bending vibration δNH of the amide I mode of the peptide bond (-NHCO-) of proteins [1,21,22]. The Amide I band is a combination band of the stretching vibration vC-N group and the in-plane bending vibration δN-H of the peptide bond and is very sensitive to changes in both the environment and the conformations of the protein chains depending on the form of collagen and on the state of the disease [1,6,[17][18][19][20][21][22]. This band shifts to lower frequencies in the spectra of diabetic mice D20 and D30 suggesting the These results were confirmed upon the comparison of dielectric constant of the skin.…”

Section: Experimental Groupsmentioning

confidence: 99%

The Influence of UV Irradiation on Diabetic Mice Skin: A Vibrational FT-IR and Raman Spectroscopic Study

Anastassopoulou¹,

Kyriakidou²,

Mamareli³

et al. 2019

Chromatogr Spectrosc Tech

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The aim of this study was to investigate the effect of UV irradiation on streptozotocin-induced diabetic skin. 42 hairless male mice were divided into three groups of control non-diabetic and with dose of streptozotocin 20 and 30 mg/kg. Both FT-IR and Raman spectra showed considerable changes between the diabetic and non-diabetic mice, as well as after irradiation. The most important changes between healthy and diabetic groups were observed in the reduction of vOH intensity absorption bands (4000-3600 cm -1 ), resulting from dehydration of the skin and hyaluronic acid degradation. The increase of the absorption bands of symmetric and antisymmetric methylene groups in the spectral region 3000-2850 cm -1 is related to the increased lipophilic environment of diabetic mice skin. The intensity of these bands increases further upon irradiation. The characteristic "marker band" for the oxidative stress at 1744 cm -1 , due to malondialdehyde formation, increases upon irradiation and it is higher in diabetic tissues. The absorption bands of amide I at 1650 cm -1 and amide II at 1550 cm -1 shift to higher and lower frequencies, respectively, concerning the production of amyloid like proteins, in agreement with the increase of the lipophilic environment. These shifts are more pronounced in infrared spectra. The band at 1171 cm -1 , assigned to C-O-C vibration, increases upon diabetes and skin cancer development. It is important to notice that the mice who received 30 mg/kg streptozotocin showed high resistance to UV irradiation. It seems that streptozotocin inhibits the free radicals, which are produced by UV radiation, to interact with important biological molecules. The protective role of streptozotocin on diabetic mice skin from UV irradiation are unclear. The above observations were in agreement with clinical and literature data.

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Section: Experimental Groupsmentioning

confidence: 99%

The Influence of UV Irradiation on Diabetic Mice Skin: A Vibrational FT-IR and Raman Spectroscopic Study

Anastassopoulou¹,

Kyriakidou²,

Mamareli³

et al. 2019

Chromatogr Spectrosc Tech

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“…Various aspects of AV research can be investigated using FT-IR spectroscopy to cover basic science and materials research but also in vitro substance testing analyses [14,[19][20][21][22][23][24]35,48]. Pathological human AV tissue niches with different environmental compositions can exhibit diverse types of apatite and precursors as well as changes in ECM matrix composition and conformation [49].…”

Section: Discussionmentioning

confidence: 99%

“…So far, Fourier transformed infrared (FT-IR) spectroscopy of human AV tissue has mainly been used to investigate hydroxyapatite (HAP) features without imaging tissue sections in larger two-dimensional areas [19][20][21][22][23][24]. Mineral AV deposits punctually analysed are composed of crystalline, amorphous inorganic calcium phosphates and low crystallinity biological hydroxyapatite [19][20][21][22]25].…”

Section: Introductionmentioning

confidence: 99%

“…However, the chemical definition of ECM composition and collagen conformation is of special relevance because changes in the protein matrix during fibrotic AV thickening or by treatment during prostheses fabrication may be causing this pathological biomineralization. The transition of the collagen conformation towards a higher β-sheet and random coil structure or changes in the collagen vs. elastin content are selectively monitored using FT-IR spectroscopy without imaging, and they are important aspects of the pathological in vivo AV situation [19,23,24,39]. Mechanical aspects can affect these IR spectroscopic characteristics.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Spectroscopic Imaging Offers a Systematic Assessment of Pathological Aortic Valve and Prosthesis Tissue in Biomineralization

et al. 2020

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Pathological ECM remodelling and biomineralization in human aortic valve and bioprosthesis tissue were investigated by Fourier transformed infrared (FT-IR) spectroscopic imaging and multivariate data analysis. Results of histological von Kossa staining to monitor hydroxyapatite biomineralization correlated to the definition of mineralized tissue using FT-IR spectroscopic imaging. Spectra exhibit signals of carbonate and phosphate groups of hydroxyapatite. Proteins could be identified by the amide I and amide II bands. Proteins were detected in the calcified human aortic valve tissue, but no absorption signals of proteins were observed in the mineralized bioprosthesis sample region. A shift of the amide I band from 1654 cm−1 to 1636 cm−1 was assumed to result from β-sheet structures. This band shift was observed in regions where the mineralization process had been identified but also in non-mineralized bioprosthesis tissue independent of prior implantation. The increased occurrence of β-sheet conformation is hypothesized to be a promoter of the biomineralization process. FT-IR spectroscopic imaging offers a wealth of chemical information. For example, slight variations in band position and intensity allow investigation of heterogeneity across aortic valve tissue sections. The exact evaluation of these properties and correlation with conventional histological staining techniques give insights into aortic valve tissue remodelling and calcific pathogenesis.

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“…These bands are very characteristic and are observed in many diseases related to oxidative stress. After washing the biopsies in hexane, a non-polar solvent, it was found that the intensity decrease of the amide I and II bands was associated with the decrease of ApoI/ApoII ratio, which correspond to HDL and control LDL [27]. the stretching vibrations of vOH of water and saccharide molecules and vNH groups of proteins in the cell [14,15,23,24].…”

Section: Region 1800-1500 CM -1mentioning

confidence: 99%

The role of oxidative stress on molybdenum enzymes and ischemic reperfusion injury in hyperuricaemic patients. An infrared spectroscopic study

Mamareli¹,

Tanis²,

Anastassopoulou³

et al. 2019

Biomed Res Clin Prac

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There are many clinical evidences that hyperuricemia is a risk factor for the development of peripheral carotid and coronary vascular diseases. However, the mechanism of elevated uric acid concentration in biological systems is not yet clear. In the present work Fourier transform infrared (FT-IR) spectroscopy was used to evaluate the mechanism of calcification and plaque formation in carotid arteries in hyperuricaemic patients. Comparison between the spectra of carotid arteries from patients with elevated uric acid values and spectra obtained from patients with normal uric acid values showed structural changes of the characteristic spectral bands in the region 4000-500 cm -1 . These changes were related with changes in the concentration of the serum uric acid and the clinical history of the patients. The intensity decrease of the infrared bands in the region 1650-1500 cm -1 was associated with the decrease of the apolipoprotein ratio, ApoI/ApoII, which corresponds to HDL (High Density Lipoproteins) and the regulation of the LDL (Low Density Lipoproteins), which are related to oxidation stress. The infrared band at 1467 cm -1 indicated the presence of urea components as a result of the metabolic pathway. The shape and the intensity of the bands between 1250-900 cm -1 depend on the glycation-end products of the diseases. SEM-EDX chemical analysis showed fibril formation and molybdenum release in hyperuricaemic patients.

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FT-IR spectroscopic study of amyloid protein formation and aortic valve calcification

Cited by 7 publications

References 6 publications

The Influence of UV Irradiation on Diabetic Mice Skin: A Vibrational FT-IR and Raman Spectroscopic Study

The Influence of UV Irradiation on Diabetic Mice Skin: A Vibrational FT-IR and Raman Spectroscopic Study

Molecular Spectroscopic Imaging Offers a Systematic Assessment of Pathological Aortic Valve and Prosthesis Tissue in Biomineralization

The role of oxidative stress on molybdenum enzymes and ischemic reperfusion injury in hyperuricaemic patients. An infrared spectroscopic study

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