FT-IR study of pectate and pectinate gels formed by divalent cations

Wellner, Nikolaus; Kačuráková, Marta; Malovı́ková, Anna; Wilson, Reginald H.; Belton, Peter

doi:10.1016/s0008-6215(98)00065-2

Cited by 104 publications

(55 citation statements)

References 24 publications

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“…These changes in cellulose and xyloglucan peak energy suggest an altered hydrogen bonding environment along cellulose microfibrils. The shoulder at ‫6241ف‬ cm Ϫ1 seen in pmr6-1 arises from free carboxyl stretching of pectins (Wellner et al, 1998). The spikes present in both spectra are the result of water vapor above the samples.…”

Section: Discussionmentioning

confidence: 92%

“…Wavenumbers of features deviating from the mean centered spectra are shown. (B) The first principal component suggests that pmr6-1 cell walls are enriched in pectins with a lower degree of esterification than the wild type, as shown by the features at 1609 cm Ϫ1 (Fillipov, 1972;Coimbra et al, 1998), 1426 cm Ϫ1 (Wellner et al, 1998), 1101 cm Ϫ1 (Coimbra et al, 1998), and 1018 cm Ϫ1 (Coimbra et al, 1998;Wellner et al, 1998). The loading feature at 1426 cm Ϫ1 has two equally plausible assignments: (1) a carboxylate stretch of pectate, or (2) cellulose with altered intermolecular hydrogen bonding (Cael et al, 1975;Kataoka and Kondo, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…The loading feature at 1426 cm Ϫ1 has two equally plausible assignments: (1) a carboxylate stretch of pectate, or (2) cellulose with altered intermolecular hydrogen bonding (Cael et al, 1975;Kataoka and Kondo, 1998). (C) The second principal component suggests that epidermis is slightly enriched for protein (1669 and 1553 cm Ϫ1 ) and pectin (1114 and 1074 cm Ϫ1 ) (Wellner et al, 1998). (D) Biplot showing the separation of Col and pmr6-1 midinfrared spectra generated by the covariance-matrix approach for principal components analysis (PCA) (Kemsley, 1998).…”

Section: Discussionmentioning

confidence: 99%

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PMR6, a Pectate Lyase–Like Gene Required for Powdery Mildew Susceptibility in Arabidopsis

et al. 2002

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The plant genes required for the growth and reproduction of plant pathogens are largely unknown. In an effort to identify these genes, we isolated Arabidopsis mutants that do not support the normal growth of the powdery mildew pathogen Erysiphe cichoracearum . Here, we report on the cloning and characterization of one of these genes, PMR6 . PMR6 encodes a pectate lyase-like protein with a novel C-terminal domain. Consistent with its predicted gene function, mutations in PMR6 alter the composition of the plant cell wall, as shown by Fourier transform infrared spectroscopy. pmr6 -mediated resistance requires neither salicylic acid nor the ability to perceive jasmonic acid or ethylene, indicating that the resistance mechanism does not require the activation of well-described defense pathways. Thus, pmr6 resistance represents a novel form of disease resistance based on the loss of a gene required during a compatible interaction rather than the activation of known host defense pathways.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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PMR6, a Pectate Lyase–Like Gene Required for Powdery Mildew Susceptibility in Arabidopsis

et al. 2002

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“…The strong absorption of C-O stretch at 1035.6 cm −1 indicated the presence of polysaccharides [34]. The peaks at 1510 cm −1 and 1423.2 cm −1 were related to the vibration of aromatic structures in the lignin moieties [35]. FT-IR analysis confirmed that the spherical biocarriers had a typical LCC structure composed of lignin and polysaccharides.…”

Section: Ft-ir Analysismentioning

confidence: 87%

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Zhao

Wang

et al. 2017

International Journal of Polymer Science

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Spherical biocarriers were prepared with lignin-carbohydrate complexes isolated from ginkgo (Ginkgo biloba L.) xylem. The specific surface and average pore size of the biocarriers were 17.15 m 2 g −1 and 21.59 nm, respectively. The carriers were stable in solution at pH 4.0∼9.5. Fourier transform infrared (FT-IR) spectrum indicated that the spherical carrier was composed of lignin and polysaccharides and had a typical lignin-carbohydrate complex (LCC) structure. The contents of galactose, lignin, and total sugar were 3.30%, 23.9%, and 64.62%, respectively, making the spherical biocarriers have good physical strength and compatible with hepatocytes. It was observed using a scanning electron microscopy (SEM) that liver cells adhered to the spherical biocarriers during culture. Cell counting indicated that the proliferation of liver cells in the experimental group was significantly higher than that of the control group. The albumin secretion (ALB) value and glucose consumption of the human hepatocytes were increased by 51.7% and 38.6%, respectively, by the fourth day when cultivated on the biocarriers. The results indicate that ginkgo LCC is very biocompatible and shows promise for the use as a biomaterial in the culture of human hepatocytes.

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“…Both spectra of pectin and sample No. 2 have additional bands at 1444, 1413 (1420), 1369 (1374), 1272 (1266) and 930 (925) cm -1 , which may be ascribed) to ester and carboxylate groups (FILIPPOV 1978;ENGELSEN & NØRGAARD 1996;WELLNER et al 1998). The band at 1333 (1338) cm -1 was assigned to C-H bending vibration of pyranoic ring.…”

Section: Resultsmentioning

confidence: 99%

Application of FT-IR spectroscopy in detection of food hydrocolloids in confectionery jellies and food supplements

Čopı́ková¹,

Synytsya²,

Černá³

et al. 2001

Czech J. Food Sci.

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FT-IR study of pectate and pectinate gels formed by divalent cations

Cited by 104 publications

References 24 publications

PMR6, a Pectate Lyase–Like Gene Required for Powdery Mildew Susceptibility in Arabidopsis

PMR6, a Pectate Lyase–Like Gene Required for Powdery Mildew Susceptibility in Arabidopsis

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Application of FT-IR spectroscopy in detection of food hydrocolloids in confectionery jellies and food supplements

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