FT-NIR: a tool for rapid intracellular lipid quantification in oleaginous yeasts

Chmielarz, Mikołaj; Sampels, Sabine; Blomqvist, Johanna; Brandenburg, Jule; Wende, Frida J.; Sandgren, Mats; Passoth, Volkmar

doi:10.1186/s13068-019-1513-9

Cited by 17 publications

(12 citation statements)

References 28 publications

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“…The C/N ratio of HH10CG55 mixture was 53.6, and CG50 C/N ratio was 48 which are slightly below the reported optimum for lipid production of 60-100 [32]. To obtain an impression on the lipid accumulation kinetics, the intracellular lipid content during fermentation was checked over the whole fermentation time using our recently established FT-NIR-based method for intracellular lipid determination [33].…”

Section: Fermentation In Bioreactorsmentioning

confidence: 99%

Microbial lipid production from crude glycerol and hemicellulosic hydrolysate with oleaginous yeasts

Chmielarz

Blomqvist

Sampels

et al. 2021

Biotechnol Biofuels

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Background Crude glycerol (CG) and hemicellulose hydrolysate (HH) are low—value side-products of biodiesel transesterification and pulp—and paper industry or lignocellulosic ethanol production, respectively, which can be converted to microbial lipids by oleaginous yeasts. This study aimed to test the ability of oleaginous yeasts to utilise CG and HH and mixtures of them as carbon source. Results Eleven out of 27 tested strains of oleaginous yeast species were able to grow in plate tests on CG as sole carbon source. Among them, only one ascomycetous strain, belonging to Lipomyces starkeyi, was identified, the other 10 strains were Rhodotorula spec. When yeasts were cultivated in mixed CG/ HH medium, we observed an activation of glycerol conversion in the Rhodotorula strains, but not in L. starkeyi. Two strains—Rhodotorula toruloides CBS 14 and Rhodotorula glutinis CBS 3044 were further tested in controlled fermentations in bioreactors in different mixtures of CG and HH. The highest measured average biomass and lipid concentration were achieved with R. toruloides in 10% HH medium mixed with 55 g/L CG—19.4 g/L and 10.6 g/L, respectively, with a lipid yield of 0.25 g lipids per consumed g of carbon source. Fatty acid composition was similar to other R. toruloides strains and comparable to that of vegetable oils. Conclusions There were big strain differences in the ability to convert CG to lipids, as only few of the tested strains were able to grow. Lipid production rates and yields showed that mixing GC and HH have a stimulating effect on lipid accumulation in R. toruloides and R. glutinis resulting in shortened fermentation time to reach maximum lipid concentration, which provides a new perspective on converting these low-value compounds to microbial lipids.

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Section: Fermentation In Bioreactorsmentioning

confidence: 99%

Microbial lipid production from crude glycerol and hemicellulosic hydrolysate with oleaginous yeasts

Chmielarz

Blomqvist

Sampels

et al. 2021

Biotechnol Biofuels

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“…Lipid concentration and fatty acid profile were determined as described earlier [6]. For rapid determination of lipid content we used our recently described FT-NIR method [20].…”

Section: Methodsmentioning

confidence: 99%

“…This was carried out to achieve a more optimal C/N ratio [19]. To obtain an impression on the lipid accumulation kinetics, the intracellular lipid content during fermentation was checked over the whole fermentation time using our recently established FT-NIR-based method for intracellular lipid determination [20]. In the cultivation of R. toruloides CBS 14 with HH the highest cell dry weight (21.2 g/L) and lipid concentration (12.5 g/L) were reached after 96 h cultivation ( Figure 6B).…”

Section: Fermentation In Bioreactorsmentioning

confidence: 99%

Bio-lipid production from crude glycerol and lignocellulosic hydrolysate with oleaginous yeasts

Chmielarz¹,

Blomqvist²,

Sampels³

et al. 2020

Preprint

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Background: Crude glycerol (CG) and hemicellulose hydrolysate (HH) are low- value side-products of biodiesel transesterification and pulp- and paper industry, respectively, which can be converted to microbial lipids by oleaginous yeasts. This study aimed to test the ability of oleaginous yeasts to utilise CG and HH and mixtures of them as carbon source. Results: Eleven out of 27 tested strains of oleaginous yeast species were able to grow in plate tests on CG as sole carbon source. Among them, only one ascomycetous strain, belonging to Lipomyces starkeyi , was identified, the other 10 strains were Rhodotorula spec. When yeasts were cultivated in mixed CG/ HH medium, we observed an activation of glycerol conversion in the Rhodotorula strains, but not in L. starkeyi . Two strains - Rhodotorula toruloides CBS 14 and Rhodotorula glutinis CBS 3044 were further tested in controlled fermentations in bioreactors in different mixtures of CG and HH. The highest measured average biomass and lipid concentration were achieved with R. toruloides in 40% HH medium mixed with 60 g/L CG - 19.4 g/L and 10.6 g/L, respectively, with a lipid yield of 0.22 g lipids per consumed g of carbon source. Fatty acid composition was similar to other R. toruloides strains and comparable to that of vegetable oils. Conclusions: There were big strain differences in the ability to convert CG to lipids, as only few of the tested strains were able to grow. Lipid production rates and yields showed that mixing GC and HH have a stimulating effect on lipid accumulation resulting in shortened fermentation time to reach maximum lipid concentration, which provides a new perspective on converting these low- value compounds to biolipids.

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“…Lipid accumulation in intact cells of L. starkeyi grown in SBP hydrolysate added with molasses was monitored over time by Fourier Transform Infrared (FTIR) micropectroscopy. FTIR is an e cient tool for rapidly following up microbial lipid accumulation at different stages of growth [32][33][34][35][36][37][38][39], since it can analyze intact cells identifying speci c molecular groups by their absorption bands. Starting from the spectra obtained sampling the cultivations over time, Figure 4 illustrates the temporal evolution of the CHx stretching band area, between 3050 and 2800 cm −1 , and of the ester carbonyl band area, between 1760 and 1730 cm −1 , after normalization for the total protein content, given by the amide I band area ( Figure S1 in Additional le).…”

Section: Growth and Lipid Accumulation In Sbp Hydrolysate Blended Witmentioning

confidence: 99%

Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production

Martani

Maestroni

Torchio

et al. 2020

Preprint

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Background: Lipids from oleaginous yeasts emerged as a sustainable alternative to vegetable oils and animal fat to produce biodiesel, the biodegradable and environmentally friendly counterpart of petro-diesel fuel. To develop economically viable microbial processes, the use of residual feedstocks as growth and production substrates is required.Results: In this work we investigated sugar beet pulp (SBP) and molasses, the main residues of sugar beet processing, as sustainable substrates for the growth and lipid accumulation by the oleaginous yeast Lipomyces starkeyi. We observed that in hydrolysed SBP the yeast cultures reached a limited biomass, cellular lipid content, lipid production and yield (2.5 g/L, 19.2 %, 0.5 g/L and 0.08 g/g, respectively). To increase the initial sugar availability, cells were grown in SBP blended with molasses. Under batch cultivation, the cellular lipid content was more than doubled (47.2 %) in the presence of 6 % molasses. Under pulsed-feeding cultivation, final biomass, cellular lipid content, lipid production and lipid yield were further improved, reaching respectively 20.5 g/L, 49.2 %, 9.7 g/L and 0.178 g/g. Finally, we observed that SBP can be used instead of ammonium sulphate to fulfil yeasts nitrogen requirement in molasses-based media for microbial oil production. Conclusions: This study demonstrates for the first time that SBP and molasses can be blended to create a feedstock for the sustainable production of lipids by L. starkeyi. The data obtained pave the way to further improve lipid production by designing a fed-batch process in bioreactor.

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FT-NIR: a tool for rapid intracellular lipid quantification in oleaginous yeasts

Cited by 17 publications

References 28 publications

Microbial lipid production from crude glycerol and hemicellulosic hydrolysate with oleaginous yeasts

Microbial lipid production from crude glycerol and hemicellulosic hydrolysate with oleaginous yeasts

Bio-lipid production from crude glycerol and lignocellulosic hydrolysate with oleaginous yeasts

Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production

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