FTIR-assisted MALDI-TOF MS for the identification and typing of bacteria

Feng, Bin; Shi, Haimei; Xu, Fuxing; Hu, Fupin; He, Jian; Yang, Huayan; Ding, Chuan‐Fan; Chen, Wenxiang; Yu, Shaoning

doi:10.1016/j.aca.2020.03.037

Cited by 44 publications

(33 citation statements)

References 42 publications

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“…The MALDI-TOF MS approach, which establishes bacteria identification based on proteomic profiles, is likely unable to detect low differences at the genomic level, and thus returned no results. Different studies in the fields of bacteria identification suggest to combine MALDI Biotyper system analysis with Fourier-transfrom infrared spectroscopy (FTIR), which can be used to analyze carbohydrate and glycoproteins compounds, is able to provide a unique "fingerprint" spectrum for each species of bacteria exceeding the limit previously described (Maity et al, 2013;Feng et al, 2020). The three positive controls, belonging to different serogroups (i.e., 1, 3, and 6), were identified only at the species level without serogroup identification, confirming that the MALDI Biotyper system is unable to discriminate between L. pneumophila among serogroups.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of MALDI–TOF Mass Spectrometry in Diagnostic and Environmental Surveillance of Legionella Species: A Comparison With Culture and Mip-Gene Sequencing Technique

et al. 2020

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Legionella spp. are widespread bacteria in aquatic environments with a growing impact on human health. Between the 61 species, Legionella pneumophila is the most prevalent in human diseases; on the contrary, Legionella non-pneumophila species are less detected in clinical diagnosis or during environmental surveillance due to their slow growth in culture and the absence of specific and rapid diagnostic/analytical tools. Reliable and rapid isolate identification is essential to estimate the source of infection, to undertake containment measures, and to determine clinical treatment. Matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI–TOF MS), since its introduction into the routine diagnostics of laboratories, represents a widely accepted method for the identification of different bacteria species, described in a few studies on the Legionella clinical and environmental surveillance. The focus of this study was the improvement of MALDI–TOF MS on Legionella non-pneumophila species collected during Legionella nosocomial and community surveillance. Comparative analysis with cultural and mip-gene sequencing results was performed. Moreover, a phylogenetic analysis was carried out to estimate the correlations amongst isolates. MALDI–TOF MS achieved correct species-level identification for 45.0% of the isolates belonging to the Legionella anisa, Legionella rubrilucens, Legionella feeleii, and Legionella jordanis species, displaying a high concordance with the mip-gene sequencing results. In contrast, less reliable identification was found for the remaining 55.0% of the isolates, corresponding to the samples belonging to species not yet included in the database. The phylogenetic analysis showed relevant differences inside the species, regruped in three main clades; among the Legionella anisa clade, a subclade with a divergence of 3.3% from the main clade was observed. Moreover, one isolate, identified as Legionella quinlivanii, displayed a divergence of 3.8% from the corresponding reference strain. However, these findings require supplementary investigation. The results encourage the implementation of MALDI–TOF MS in routine diagnostics and environmental Legionella surveillance, as it displays a reliable and faster identification at the species level, as well as the potential to identify species that are not yet included in the database. Moreover, phylogenetic analysis is a relevant approach to correlate the isolates and to track their spread, especially in unconventional reservoirs, where Legionella prevention is still underestimated.

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Section: Discussionmentioning

confidence: 99%

Evaluation of MALDI–TOF Mass Spectrometry in Diagnostic and Environmental Surveillance of Legionella Species: A Comparison With Culture and Mip-Gene Sequencing Technique

et al. 2020

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“…140 In both of these methods, the analyst is comparing and matching distinguished and discriminated peak (biomarker) in the MALDI-MS profiles of the bacteria under investigation with publicly available databases. 141,142 The use of MALDI-MS has been extended to a variety of research areas, including the direct analysis of organisms and biological tissue samples and the characterisation of peptide and protein components. [143][144][145] In addition to its qualitative applications, MALDI-MS is also a popular quantitative tool due to its ability to analyse molecules over a wide range of mass, its high sensitivity, easy and fast sample preparation, and short analysis time.…”

Section: An Introduction To Matrix-assisted Laser Desorption/ionisatimentioning

confidence: 99%

Discrimination of bacteria using whole organism fingerprinting: the utility of modern physicochemical techniques for bacterial typing

AlMasoud

Muhamadali

Chisanga

et al. 2021

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“…In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been introduced into clinical microbiology laboratories. Due to the specificity, speed of analysis, and low cost of consumables, MALDI-TOF MS has been widely acclaimed for clinical bacterial identification (Welker et al, 2019;Feng et al, 2020;Nomura et al, 2020;Papagiannopoulou et al, 2020). MALDI-TOF MS identification is mainly based on the MS fingerprint pattern of bacterial ribosomal proteins in the m/z range of 2-20 kDa, which can be compared to the MS database to identify the bacterial genus and species (Singhal et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…MALDI-TOF MS identification is mainly based on the MS fingerprint pattern of bacterial ribosomal proteins in the m/z range of 2-20 kDa, which can be compared to the MS database to identify the bacterial genus and species (Singhal et al, 2015). MALDI-TOF MS databases with more than 4000 strains have been set up and are widely used in the identification of clinical bacteria, fungi, mycobacteria, and Nocardia (Feng et al, 2020). Nevertheless, the single colonies are always needed for this method.…”

Section: Introductionmentioning

confidence: 99%

Application of MALDI-TOF MS Profiling Coupled With Functionalized Magnetic Enrichment for Rapid Identification of Pathogens in a Patient With Open Fracture

et al. 2021

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Posttraumatic infections can occur in orthopedic trauma patients, especially in open fractures. Rapid and accurate identification of pathogens in orthopedic trauma is important for clinical diagnosis and antimicrobial treatment. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been successfully used for first-line identification of pathogens grown on culture plates. However, for direct analysis of liquid clinical specimens, pre-purification of the sample is necessary. Herein, we investigated the feasibility of coupling Fc-MBL@Fe3O4 enrichment with MALDI-TOF MS profiling in the identification of pathogens in liquid-cultured samples. This method is successfully used for the identification of pathogens in a patient with an open-leg fracture obtained at sea. Pathogens were enriched by Fc-MBL@Fe3O4 from briefly pre-cultured liquid media and identified by MALDI-TOF MS. We identified an opportunistic pathogen, Vibrio alginolyticus, which is uncommon in clinical orthopedic trauma infection but exists widely in the sea. Therefore, combining Fc-MBL@Fe3O4 enrichment and MALDI-TOF MS profiling has great potential for direct identification of microbes in clinical samples.

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FTIR-assisted MALDI-TOF MS for the identification and typing of bacteria

Cited by 44 publications

References 42 publications

Evaluation of MALDI–TOF Mass Spectrometry in Diagnostic and Environmental Surveillance of Legionella Species: A Comparison With Culture and Mip-Gene Sequencing Technique

Evaluation of MALDI–TOF Mass Spectrometry in Diagnostic and Environmental Surveillance of Legionella Species: A Comparison With Culture and Mip-Gene Sequencing Technique

Discrimination of bacteria using whole organism fingerprinting: the utility of modern physicochemical techniques for bacterial typing

Application of MALDI-TOF MS Profiling Coupled With Functionalized Magnetic Enrichment for Rapid Identification of Pathogens in a Patient With Open Fracture

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