FTIR-ATR detection of proteins and small molecules through DNA conjugation

Liao, Wei; Fang, Wei; Liú, Dan; Qian, Min; Yuan, Gu; Zhao, Xin

doi:10.1016/j.snb.2005.06.021

Cited by 49 publications

(36 citation statements)

References 35 publications

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“…[1][2][3][4][5][6][7] In ATR, an evanescent field is set up by the electromagnetic radiation that is totally and internally reflected at the interface between the crystal where the IR light is propagating (the so called internal reflection element, IRE) and the medium above the IRE. Since the evanescent field decays exponentially away from the interface, an ATR measurement only picks up signals within a few microns from the IRE, depending on angle of incidence and the ratio of the refractive index of the IRE and the contacting media.…”

Section: Introductionmentioning

confidence: 99%

“…The versatility of ATR-FTIR spectroscopy is reflected in the literature, which covers a wide range of topics. Pertinent to the present study are the applications in the biological sciences, such as biosensing [2,4,5] and antibody recognition events, [3] in situ monitoring of bilayer formation, [6,12] surface concentration determination, [3,4,7,13] detection of protein…”

Section: Introductionmentioning

confidence: 99%

“…[7,20,21] Very recently some progress has also been made to functionalise IREs with crystals based on germanium [3,4,22] and silicon. [2,23] An appropriately functionalised IRE can be used as a biosensor, for example, or for protein fishing. However, it is still a challenge to achieve a versatile sensing device based on ATR-FTIR spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

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A Novel ATR‐FTIR Approach for Characterisation and Identification of Ex Situ Immobilised Species

et al. 2007

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We demonstrate a novel method to analyse ex situ prepared protein chips by attenuated total reflection Fourier IR spectroscopy (ATR‐FTIR), which circumvents tedious functionalisation steps of internal reflection elements (IREs), and simultaneously allows for complementary measurements by other analytical techniques. This concept is proven by utilising immobilised metal affinity capture (IMAC™) chips containing about 10 μm thick films of copolymers coated with nitrilotriacetic acid (NTA) groups, which originally was manufactured for surface enhanced laser desorption ionisation (SELDI) spectrometry. Three immobilisation steps were analysed by ATR‐FTIR spectroscopy: 1) NTA complexation with nickel(II) ions 2) binding of two histidine (His)‐tagged synthetic peptides of 25 (25‐His6) and 48 (48‐His6) amino acids to the NTA‐groups and 3) attachment of a ligand, mesyl amide, to the surface‐bound 48‐His6. Despite interference from H2O, both amide I and II were well resolved. Utilising peptide adsorption in the thick copolymer matrix yields a high saturation peptide concentration of ≈100 mg mL−1 and a dissociation constant of 116±11 μM, as determined by a detailed analysis of the Langmuir adsorption isotherm. The mesyl amide ligand was directly seen in the raw ATR‐FTIR spectrum with specific peaks in the fingerprint region at 1172 and 1350 cm−1. Several aspects of the fine structure of the amide I band of the peptide were analysed: influences from secondary structure, amino side chains and competing contamination product. We believe that this approach has great potential as a stand‐alone or complementary analytical tool for determination of the chemical composition of functionalised surfaces. We emphasise further that with this approach no chemical treatment of IREs is needed; the chips can be regenerated and reused, and applied in other experimental set‐ups.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Novel ATR‐FTIR Approach for Characterisation and Identification of Ex Situ Immobilised Species

et al. 2007

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“…Numerous examples of biodetection using organic monolayers have been described: for example, those based on fluorescence [Cattaruzza, et al, 2006], Raman scattering [Yonzon, et al, 2004], ellipsometry [Arwin, 2000[Arwin, & 2001, infra-red [Liao, et al, 2006] or simply [Masuda, et al, 2005] have used the specificity of monolayer-functionalized surfaces. The geometry of the sensor also has a remarkable importance for the realization of the devices.…”

Section: Biodetection Based On Organic Monolayers On Surfacesmentioning

confidence: 99%

Silicon and Silicon-Related Surfaces for Biosensor Applications

Arafat¹,

Daous²

2011

Environmental Biosensors

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“…Está técnica pode ser utilizada no estudo de neoplasias [47], no estudo de bactérias [61], [62] na análise de e DNA [65], [66] e de proteínas como o colágeno [63], [64], além de fornecer informações relevantes sobre diversos tipos de tecidos biológicos [60].…”

Section: Utilização Do Ftir Na Biologia E áReas Correlatasunclassified

Avaliação in vitro dos efeitos da radiação ionizante em tecido ósseo bovino por espectroscopia ATR-FTIR e análise dinâmico-mecânica

Veloso¹

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FTIR-ATR detection of proteins and small molecules through DNA conjugation

Cited by 49 publications

References 35 publications

A Novel ATR‐FTIR Approach for Characterisation and Identification of Ex Situ Immobilised Species

A Novel ATR‐FTIR Approach for Characterisation and Identification of Ex Situ Immobilised Species

Silicon and Silicon-Related Surfaces for Biosensor Applications

Avaliação in vitro dos efeitos da radiação ionizante em tecido ósseo bovino por espectroscopia ATR-FTIR e análise dinâmico-mecânica

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