FTIR microspectroscopic analysis of the effects of certain drugs on oxidative stress and brain protein structure

Carmona, Pedro; Rodrı́guez-Casado, Arantxa; Álvarez, Isabel; Miguel, Enrique de; Toledano, A

doi:10.1002/bip.20944

Cited by 18 publications

(11 citation statements)

References 30 publications

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“…Lipoperoxidation increased in the cerebellum/Medulla oblongata and hemisphere regions in co-administration of single dose of nicotine and oligoelements as well as in nicotine alone. With the use of only oligoelements, this effect decreased in these regions which did not agree with the findings of Carmona et al, [22], who suggest that nicotine attenuates the formation of peroxides, and might reduce the production of beta-amyloid proteins in Alzheimer's disease with chronic treatment doses, and may be a novel protective therapy in Alzheimer's disease [23]. With respect to the region of the cortex, there was no finding of any effect to the treatment in all the animals, although recent studies suggest that the presence of nicotine increases the formation of peroxides in this region [24].…”

Section: Discussioncontrasting

confidence: 69%

Effect of Nicotine on Dopamine and Glutathione Levels in Presence of Oligoelements in Brain Regions of Young Rats——Effect of Nicotine on Brain Regions of Rat

Guzmán¹,

García²,

Jiménez³

et al. 2012

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Aim: The purpose of this study was to understand the mechanism of nicotine mediated addiction and the role of oligoelements in reducing its effect. Methods: Male Wistar rats (weight 80 g) were treated with single and repeated doses of nicotine and/or oligoelements as follows: group 1 (control) NaCl 0.9%; group 2, nicotine (1 mg/kg); group 3, oligoelements (50 μl/rat); and group 4, nicotine (1 mg/kg) + oligoelements (50 μl/rat). All drugs were intraperitoneally administered for 4 days. Blood for the measurement of glucose was obtained from all the animals. Samples of the brain regions (cortex, hemispheres and cerebellum + medulla oblongata) of each rat were obtained and used to measure the concentrations of dopamine, GSH levels, and lipid peroxidation (TBARS) using fluorescence and spectrophotometric methods. Results: Glucose level increased in rats treated with nicotine and oligoelements (p < 0.05), while GSH level decreased in cerebellum/medulla oblongata and hemispheres (p < 0.05) of the same animals. TBARS levels increased in cerebellum/medulla oblongata and hemispheres of animals treated with nicotine and oligoelements, but decreased in the same regions (p < 0.05) in rats treated only with oligoelements. The levels of dopamine decreased in cortex and hemispheres, but increased in cerebellum/medulla and oblongata regions of rats treated with both compounds (p < 0.05). Conclusions: Nicotine and oligoelements are associated with increase in the level of glucose, an effect that was more pronounced in the group treated with both drugs. Reduction of oxidative stress and dopamine metabolism may be involved in this effect

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Section: Discussioncontrasting

confidence: 69%

Effect of Nicotine on Dopamine and Glutathione Levels in Presence of Oligoelements in Brain Regions of Young Rats——Effect of Nicotine on Brain Regions of Rat

Guzmán¹,

García²,

Jiménez³

et al. 2012

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“…[1,2] So, it has been widely applied to qualitative and quantitative analyses in chemistry, [3] biology, [4] medicinal chemistry, [5] disease diagnosis, [6,7] and environmental science, and so forth. [8] In recent years, with the rapid development of chemo metrics, computer science, and molecular biology, the study on DNA molecules by using FTIR is particularly eye-catching.…”

Section: Introductionmentioning

confidence: 99%

Fourier Transform Infrared Spectroscopy of the DNA of the Chuxiong Population ofCamellia reticulataLindl. of China

Qiu

Wang

Liu

et al. 2014

Spectroscopy Letters

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In order to cluster and identify 10 varieties of Chuxiong population of Camellia reticulata Lindl., the difference of DNA among the 10 varieties was detected by using Fourier transform infrared spectroscopy (FTIR), and then hierarchical cluster and principal component analysis based on average spectra of DNA was used to establish standard clustering and identifying model. The result indicated that (1) DNA fingerprints based on FTIR of the 10 varieties are different from each other, which is also supported by variance analysis (p < 0.05).(2) The rate of clustering of 30 samples is 100%. Likewise, the rate of identifying of 10 samples is 100% as well. (3) It was speculated based on clustering distance coefficients that Guomei (Variety 5) was formed by grafting the scion from the original old tree Xiangguo (Variety 1) to seedling from seed. The conclusion is that DNA FTIR is an effective manner of clustering and identifying Chuxiong population of C. reticulata Lindl., and can be used to calculate the origin of ancient Camellia tree.

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“…In particular, amide I band (1600-1700 cm 1 ) has been employed to evaluate structural and conformational characteristics of proteins. [38][39][40] Spectra of the -synuclein fibrils produced without ( Fig. 1(E)-(a)) and with ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Amyloid Polymorphism of α-Synuclein Induced by Active Firefly Luciferase

Yang¹,

Hong²,

Kim³

et al. 2014

Bulletin of the Korean Chemical Society

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Amyloidogenic proteins often exhibit fibrillar polymorphism through alternative assembly processes, which has been considered to have possible pathological implications. Here, firefly luciferase (LUC) is shown to induce amyloid polymorphism of -synuclein, the major constituent of Lewy bodies found in Parkinson's disease, by acting as a novel template. The drastically accelerated fibrillation kinetics of -synuclein with LUC required the nucleation center produced by the active enzyme of LUC. Fluorescent dye binding, transmission electron microscopy, and Fourier transformed infrared spectroscopy revealed the morphologically distinctive amyloid fibrils of -synuclein prepared in the absence or presence of LUC. As the altered morphological characteristics became inherent to the mature fibrils, those properties were inherited to next-generations via nucleation-dependent fibrillation process. The seed control, therefore, would be an effective means to modify amyloid fibrils with different biochemical characteristics. In addition, the LUC-directed amyloid fibrillar polymorphism also suggests that other cellular biomolecules including enzymes in general are able to diversify amyloid fibrils, which could be self-propagated with diversified biological activities, if any, inside cells.

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FTIR microspectroscopic analysis of the effects of certain drugs on oxidative stress and brain protein structure

Cited by 18 publications

References 30 publications

Effect of Nicotine on Dopamine and Glutathione Levels in Presence of Oligoelements in Brain Regions of Young Rats——Effect of Nicotine on Brain Regions of Rat

Effect of Nicotine on Dopamine and Glutathione Levels in Presence of Oligoelements in Brain Regions of Young Rats——Effect of Nicotine on Brain Regions of Rat

Fourier Transform Infrared Spectroscopy of the DNA of the Chuxiong Population ofCamellia reticulataLindl. of China

Amyloid Polymorphism of α-Synuclein Induced by Active Firefly Luciferase

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