FTIR monitoring of the growth of the carbonate containing apatite layers from simulated and natural body fluids

Stoch, A.; Jastrzębski, Witold; Brożek, A.; Trybalska, B.; Cichocińska, M.; Szarawara, E.

doi:10.1016/s0022-2860(99)00170-2

Cited by 105 publications

(77 citation statements)

References 10 publications

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“…, seguir padrão correto (respeitando estilo de escala, posição dos dados, caixa inferior da imagem, fundo preto). and between 3000 and 3600 cm -1 , respectively with are in good agreement with other studies [22][23][24][25][26] . The related results indicate the apatite formation on silicon nitride surfaces after treatment with the biomimetic method.…”

Section: Resultssupporting

confidence: 93%

Hydroxyapatite coating on silicon nitride surfaces using the biomimetic method

Silva¹,

Rigo

Marchi

et al. 2008

Mat. Res.

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Silicon nitride based ceramics are promising candidates for biomedical applications due to their chemical and dimensional stability associated to suitable mechanical strength and relatively high fracture toughness. However, the bioinert characteristics of these ceramics limit their application to situations where the formation of chemical bonds between the material and the tissue are not essential. A way to broaden the application field of these ceramics in medicine is promoting their bioactivity by means of a hydroxyapatite coating. Therefore, in this paper, samples of silicon nitride were coated with apatite using the biomimetic method. The treated silicon nitride surface was characterized by diffuse reflectance infrared Fourier transformed, X ray diffraction and scanning electron microscopy. The results showed that a layer of hydroxyapatite could be deposited by this method on silicon nitride samples surface.

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Section: Resultssupporting

confidence: 93%

Hydroxyapatite coating on silicon nitride surfaces using the biomimetic method

Silva¹,

Rigo

Marchi

et al. 2008

Mat. Res.

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“…After the sintering, DAp and HAp samples were mechanically polished by #2000 emery paper to avoid the effect of surface roughness 7) and then soaked in a simulated body fluid (SBF) at 309.5 K. Table 1 represents the chemical composition of SBF used in the present study. 8) A bone-like layer formed on DAp and HAp, which was reported to be poorly crystallized carbonated apatite 9) was observed by a scanning electron microscope (SEM) and a transmission . Statistical analysis of the data was done by Student's t-test, and the differences in the data were considered to be significant when p < 0:05.…”

Section: Methodsmentioning

confidence: 99%

Bone-like Layer Growth and Adhesion of Osteoblast-like Cells on Calcium-deficient Hydroxyapatite Synthesized at Different pH

et al. 2004

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Calcium-deficient hydroxyapatite (DAp) was synthesized by hydrolysis of -TCP at different pH. Ca/P ratio of DAp increased with increasing pH during the hydrolysis. In addition, DAp which was synthesized at lower pH, showed higher solubility in the simulated body fluid (SBF) and had a more negatively charged surface than that at higher pH. Bone-like crystals were formed on DAp which have been soaked in SBF at 309.5 K in vitro. DAp demonstrated faster formation of a bone-like layer than stoichiometric hydroxyapatite (HAp). Moreover, the formation of the crystals on DAp synthesized at lower pH was much faster than that at higher pH. The adhesion of MC3T3-E1 osteoblast-like cells was also influenced by pH during the synthesis of DAp; the number of adhering cells increased with a decrease in the pH. Therefore, high solubility and negative charge of DAp, especially that synthesized at lower pH resulted in fast formation of bone-like crystals and good cell adhesion.

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“…7) Change in calcium concentration in SBF solution with soaking time was measured by an atomic absorption spectrometer (AAS). A bone-like layer formed on HAp, which was reported to be poorly crystallized carbonated apatite, 8) was observed by a scanning electron microscope (SEM). MC3T3-E1 osteoblast-like cells were cultured in Dulbecco's modified eagle medium (DMEM) in an incubator containing 10% CO 2 at 310 K. The suspension of MC3T3-E1 cells was placed in a 96-well culture microplate with HAp/-TCP composites.…”

Section: Methodsmentioning

confidence: 99%

Effect of β-TCP Size on Bone-Like Layer Growth and Adhesion of Osteoblast-Like Cells in Hydroxyapatite/β-TCP Composites

et al. 2006

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Hydroxyapatite (HAp)-20 vol% -tricalcium phosphate (-TCP) composites were prepared. The effect of -TCP size on bone-like layer growth and adhesion of osteoblast-like cells on the composites was systematically studied in vitro. When the composites were soaked in a simulated body fluid, the formation and growth rates of the bone-like layer increased with increasing -TCP size, even if the volume fraction of -TCP was constant. Moreover, selective dissolution of -TCP phase and formation of the bone-like layer around the phase were frequently observed. Higher Ca 2þ concentration due to the fast dissolution of -TCP beneath the sample surface resulted in faster formation and growth of the bone-like layer, especially in the samples containing -TCP powders larger than 100 mm. MC3T3-E1 osteoblast-like cells preferentially adhered to -TCP phase in HAp/-TCP composites because of the enrichment of Ca 2þ ions around -TCP.

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FTIR monitoring of the growth of the carbonate containing apatite layers from simulated and natural body fluids

Cited by 105 publications

References 10 publications

Hydroxyapatite coating on silicon nitride surfaces using the biomimetic method

Hydroxyapatite coating on silicon nitride surfaces using the biomimetic method

Bone-like Layer Growth and Adhesion of Osteoblast-like Cells on Calcium-deficient Hydroxyapatite Synthesized at Different pH

Effect of β-TCP Size on Bone-Like Layer Growth and Adhesion of Osteoblast-Like Cells in Hydroxyapatite/β-TCP Composites

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FTIR monitoring of the growth of the carbonate containing apatite layers from simulated and natural body fluids

Cited by 105 publications

References 10 publications

Hydroxyapatite coating on silicon nitride surfaces using the biomimetic method

Hydroxyapatite coating on silicon nitride surfaces using the biomimetic method

Bone-like Layer Growth and Adhesion of Osteoblast-like Cells on Calcium-deficient Hydroxyapatite Synthesized at Different pH

Effect of &beta;-TCP Size on Bone-Like Layer Growth and Adhesion of Osteoblast-Like Cells in Hydroxyapatite/&beta;-TCP Composites

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Effect of β-TCP Size on Bone-Like Layer Growth and Adhesion of Osteoblast-Like Cells in Hydroxyapatite/β-TCP Composites