FTIR spectroscopy applied to bacterial cells as a novel method for monitoring complex biotechnological processes

Schuster, K.C.; Mertens, F.; Gapes, J. R.

doi:10.1016/s0924-2031(98)00058-7

Cited by 61 publications

(35 citation statements)

References 14 publications

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“…Changes in regions of 1407 cm À 1 could be attributed to an increasing lipid content in late culture phases. This effect was previously observed with C. acetobutylicum [ 34 ] . In the carbohydrate region ( 1200 to 900 cm À 1 ) , changes were very significant.…”

Section: Spectral Changes During Growth and Solvent Formation Of C Bsupporting

confidence: 83%

“…In an earlier study [ 34 ] , it was observed that FT-IR spectra of C. beijerinckii NRRL B592 and C. acetobutylicum change significantly during growth and solvent production in batch culture experiments. The strains [ 34 ] show a major difference to C. beijerinckii NCIMB 8052 in their physiology: they stop growing at the shift to the solventogenic phase, whereas C. beijerinckii NCIMB 8052 continues to grow throughout the whole cultivation experiment ( Figure 4 ) .…”

Section: Spectral Changes During Growth and Solvent Formation Of C Bmentioning

confidence: 98%

“…The strains [ 34 ] show a major difference to C. beijerinckii NCIMB 8052 in their physiology: they stop growing at the shift to the solventogenic phase, whereas C. beijerinckii NCIMB 8052 continues to grow throughout the whole cultivation experiment ( Figure 4 ) . However, the change in OD is not necessarily proportional to the biomass dry matter concentration due to cell differentiation, which changes the optical properties of cells.…”

Section: Spectral Changes During Growth and Solvent Formation Of C Bmentioning

confidence: 99%

“…Naumann and coworkers [ 28 ] first employed Fourier transform infrared ( FT-IR ) spectroscopy of dried films of cells in transmission mode on translucent carriers to identify organisms. It was used recently to analyse the physiological status ( acidogenic vs. solventogenic phase ) of C. acetobutylicum cultures [ 34 ] . ( The strain used there was reported to be C. beijerinckii NRRL B592, but it turned out later that one experiment has been actually conducted with C. acetobutylicum.…”

Section: Introductionmentioning

confidence: 99%

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Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells

Schuster

Goodacre

Gapes

et al. 2001

Journal of Industrial Microbiology and Biotechnology

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Strain degeneration in solventogenic clostridia is a known problem in the technical acetone -butanol fermentation bioprocess, especially in the continuous process mode. Clostridial strain degeneration was studied by Fourier transform infrared ( FT -IR ) spectroscopy of the bacterial cells. Degenerative variant formation in two strains, Clostridium beijerinckii NCIMB 8052 and Clostridium species AA332, was detected spectroscopically. Colonies on solid media were sampled, or assayed directly in situ by IR microscopy. It has previously been shown that the distinctive acidogenic and solventogenic physiological phases of Clostridium acetobutylicum in liquid medium can be discriminated by FT -IR spectroscopy. This was confirmed here for C. beijerinckii NCIMB 8052. The proportion of degenerate cells in a mixed population in liquid medium could be quantified, as the spectral features change in different ways during the normal growth cycle of wild type organisms and degenerate variants in batch culture. This opens a new perspective for physiology -based process monitoring and control, especially of the continuous acetone -butanol fermentation.

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Section: Spectral Changes During Growth and Solvent Formation Of C Bsupporting

confidence: 83%

Section: Spectral Changes During Growth and Solvent Formation Of C Bmentioning

confidence: 98%

Section: Spectral Changes During Growth and Solvent Formation Of C Bmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells

Schuster

Goodacre

Gapes

et al. 2001

Journal of Industrial Microbiology and Biotechnology

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“…This is contrary to what was seen in the exponential and stationary phases. There are minor changes in the peak at 1370 cm -1 (not identified with a lettered band in the figure, between e and f) indicating that there may be some sporulation, as was reported by Schuster et al (1999) with changes in nutrient sources. However, it is more likely that these relate to the production of carboxylic acids.…”

Section: Ft-ir Spectra Of Bacteria Samplessupporting

confidence: 58%

Cell surface acid-base properties of the cyanobacterium Synechococcus : Influences of nitrogen source, growth phase and N:P ratios

Liu

Alessi

Owttrim

et al. 2016

Geochimica et Cosmochimica Acta

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Cell surface acid-base properties of the cyanobacterium Synechococcus: Influences of nitrogen source, growth phase and N:P ratios, Geochimica et Cosmochimica Acta (2016), doi: http://dx.doi.org/10. 1016/j.gca.2016.05.023 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractThe distribution of many trace metals in the oceans is controlled by biological uptake.Recently, Liu et al. (2015) demonstrated the propensity for a marine cyanobacterium to adsorb cadmium from seawater, suggesting that cell surface reactivity might also play an important role in the cycling of metals in the oceans. However, it remains unclear how variations in cyanobacterial growth rates and nutrient supply might affect the chemical

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Vibrational Spectroscopic Studies of Microorganisms

Maquelin

Choo-Smith

Kirschner

et al. 2001

Handbook of Vibrational Spectroscopy

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The sections in this article are Introduction FT ‐ IR and R aman Spectroscopic Studies of Microorganisms Chemical Structures and Composition of Microbial Cells Main Biological Building Blocks Experimental Methodologies of IR and R aman Spectroscopy Absorption/Transmission ( A / T ) IR Spectroscopy Absorption/Reflection ( A / R ) IR Spectroscopy Attenuated Total Reflection ( ATR ) IR Spectroscopy Diffuse Reflection ( DR ) IR Spectroscopy IR Microspectroscopy Ultraviolet Resonance R aman Spectroscopy Fourier Transform R aman Spectroscopy NIR Multichannel R aman Spectroscopy Sampling of Microbial Cells Reproducibility of Measurements on Microorganisms Data Analysis Used for Characterization and Identification of Microorganisms Application Examples in Microbiology Characterization and Differentiation of Microorganisms Gram Type G + C Percentage Endospore Formation Cell Storage Materials Metabolically Released CO 2 Industrial Applications Biomedical Applications Identification Antibiotic Susceptibility Testing Reference Databases for the Identification of Microorganisms Conclusion/Future Directions

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FTIR spectroscopy applied to bacterial cells as a novel method for monitoring complex biotechnological processes

Cited by 61 publications

References 14 publications

Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells

Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells

Cell surface acid-base properties of the cyanobacterium Synechococcus : Influences of nitrogen source, growth phase and N:P ratios

Vibrational Spectroscopic Studies of Microorganisms

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