FTIR study of secondary structure changes in Epidermal Growth Factor by gold nanoparticle conjugation

Bhattacharjee, Tanmoy; Castilho, Maiara L.; Oliveira, I. R.; Jesus, V.P.S.; Hewitt, K. C.; Raniero, Leandro

doi:10.1016/j.bbagen.2017.11.009

Cited by 13 publications

(5 citation statements)

References 35 publications

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“…These DSC data were further corroborated by FTIR spectroscopic investigation of lysozyme samples. This is one of the spectroscopic techniques which has been used regularly to evaluate the secondary structures of therapeutic proteins [44][45][46]. Figure 2A shows the FTIR spectra of 7 days old showed the splitting of β sheet secondary structure (Table 2).…”

Section: Resultsmentioning

confidence: 99%

The Effects of Bisphenol A and Di-Isononyl Phthalate on Conformational Stability and Activity of Lysozyme

2018

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The purpose of this study was to investigate the effect of Bisphenol A (BPA) and Di-Isononyl Phthalate (DINP) on conformational stability and biological activity of a model protein lysozyme. Lysozyme was incubated to various concentrations (2-100 µM) of bisphenol A and phthalate for 6 weeks. The conformational stability of lysozyme was figured out by using Differential Scanning Calorimeter (DSC) and Fourier Transform Infrared (FTIR) spectrophotometer. Biological activity of the samples was determined by an enzyme activity assay using Micrococcus lysodeikticus (M. luteus) as a specific substrate to lysozyme. A rate of change of 0.001 in absorbance at 450 nm (A450 nm) was used to define 1 unit of biologically active lysozyme. The DSC data showed significant (p<0.05) decrease in Tm and H values for lysozyme samples treated with BPA or DINP with increase in exposure time. The H value of BPA treated 7 days' sample was negative which may indicate complete reversal of conformational integrity. In 7 days old DINP treated sample, no measurable peak was observed which may be due to complete loss of conformational structure. DSC data were further corroborated by FTIR and biological activity data. FTIR spectra of all the treated lysozyme samples showed the splitting of β sheet secondary structure which was completely lost in 7 days DINP treated samples which also showed the minimal biological activity. BPA and DINP (constituents of plastic bottles) may disrupt the conformational stability of protein which is expected to be more severe in human where they can remain longer than the 7 days' exposure period used in this study.

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Section: Resultsmentioning

confidence: 99%

The Effects of Bisphenol A and Di-Isononyl Phthalate on Conformational Stability and Activity of Lysozyme

2018

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“…To provide an overexpressed EGFR, it was performed on breast cancer cells, which are known as an EGFR-positive cell. This study resulted the β-sheet and loop structure in EGF protein were increased by steeps synthesis but will be decreased when the protein bind with its receptor [46].…”

Section: Gold Based Nanoparticlementioning

confidence: 89%

Nanoparticles Targeted Drug Delivery Aystem via Epidermal Growth Factor Receptor: a Review

et al. 2019

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Overexpressing of epidermal growth factor receptor (EGFR) in specific organ implicates tumour aggression and proliferation. Therefore, EGFR becomes a primary consideration for targeted cancer therapy. Nanoparticle drug delivery system is a promising multifunctional technique to provide the targeted drug delivery system. This review was aimed to discuss the nanoparticles formulation for targeted drug delivery system via EGFR. The data were collected from published journals recorded in the Scopus database. Various types of nanoparticles were widely studied to provide an EGFR-targeted drug delivery system. The formulations using specific targeting mediators were conjugated on several technologies such as polymeric nanoparticles, nanometals, and miscellaneous nanoparticles. Most of nanoparticles formulation become an excellent delivery carrier for drugs or gene to site-specific targeted by in vitro and in vivo evaluations. EGFR-targeted drug delivery system could be a promising technique to provide high effectiveness of drugs in EGFR-positive cells cancers with lower side effects to non-tumour cells.Keywords : Epidermal growth factor receptor, Cancer, Nanoparticle, targeted drug

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“…Since the interaction between rhEGF and AgNPs may modify these structures, a structural examination of the polypeptide is required to assess its potential functionality and efficacy of usage. The secondary structure of proteins and polypeptides can be followed by FTIR, specifically observing changes at the amide I region (from 1700 cm −1 to 1600 cm −1 ) [42,50], which is shown in Figure 2A. While no bands were observed in pure AgNPs, there was a rise in absorbance strength for the bands centered at 1681 cm −1 , 1666 cm −1 , 1649 cm −1 , 1635 cm −1 and 1618 cm −1 as the amount of rhEGF was increased.…”

Section: Characterization Of Ag Nanoparticles and Rhegf-coated Ag Nan...mentioning

confidence: 99%

“…Larger absorbance intensity values are expected at higher levels of rhEGF, as more loop structures and beta sheets are present (even though IR spectroscopy is not a quantitative technique, qualitative changes can be deduced). Previous studies have shown that the greater the amount of rhEGF loaded on nanoparticles, the stronger the rhEGF-rhEGF interaction that is established, resulting in fewer changes in its structure [50]. Furthermore, the presence of loop structures and β-sheets would imply better adherence of the rhEGF to the NPs, resulting in greater polypeptide stability.…”

Section: Characterization Of Ag Nanoparticles and Rhegf-coated Ag Nan...mentioning

confidence: 99%

Silver Nanoparticles Coated with Recombinant Human Epidermal Growth Factor: Synthesis, Characterization, Liberation and Anti-Escherichia coli Activity

Gonzales Matushita,

Palomino,

Rodriguez-Reyes

2023

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Epithelial tissue regeneration may be favored if the tissue receives both therapeutic agents such as recombinant human epidermal growth factor (rhEGF) and, simultaneously, antibacterial materials capable of reducing the risk of infections. Herein, we synthesized silver nanoparticles (AgNPs), which are well-known antibacterial materials, and impregnate them with rhEGF in order to study a bio-nanomaterial of potential interest for epithelial tissue regeneration. A suspension of Ag NPs is prepared by the chemical reduction method, employing sodium citrate as both a reducer and capping agent. The AgNPs suspension is mixed with a saline solution containing rhEGF, producing rhEGF-coated Ag NPs with rhEGF loadings between 0.1 and 0.4% w/w. ELISA assays of supernatants demonstrate that, in all studied cases, over 90% of the added rhEGF forms part of the coating, evidencing a high efficiency in impregnation. During the preparation of rhEGF-coated Ag NPs, no significant changes are observed on the nanoparticles, which are characterized by UV-Vis spectroscopy, transmission electron microscopy (TEM) and infrared spectroscopy. The liberation of rhEGF in vitro was followed for 72 h, finding that approximately 1% of rhEGF that is present is released. The rhEGF-coated AgNPs shows antibacterial activity against E. coli, although such activity is decreased with respect to that observed from naked AgNPs. Having confirmed the possibility of simultaneously liberating rhEGF and reducing the proliferation of bacteria, this work helps to support the use of rhEGF-loaded metallic nanoparticles for tissue regeneration.

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FTIR study of secondary structure changes in Epidermal Growth Factor by gold nanoparticle conjugation

Cited by 13 publications

References 35 publications

The Effects of Bisphenol A and Di-Isononyl Phthalate on Conformational Stability and Activity of Lysozyme

The Effects of Bisphenol A and Di-Isononyl Phthalate on Conformational Stability and Activity of Lysozyme

Nanoparticles Targeted Drug Delivery Aystem via Epidermal Growth Factor Receptor: a Review

Silver Nanoparticles Coated with Recombinant Human Epidermal Growth Factor: Synthesis, Characterization, Liberation and Anti-Escherichia coli Activity

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