FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in<i>Bacillus subtilis</i>cell division

Whitley, Kevin D.; Jukes, Calum; Tregidgo, Nicholas; Karinou, Eleni; Almada, Pedro; Henriques, Ricardo; Dekker, Cees; Holden, Séamus

doi:10.1101/2020.07.01.182006

Cited by 8 publications

(15 citation statements)

References 54 publications

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“…In contrast to ADEP, PC190723 stabilizes FtsZ bundles and halts FtsZ dynamics instead of depleting FtsZ by fast degradation as occurs in ADEP-treated cells. In line with our observations, FtsZ treadmilling had a dispensable function in accelerating septal constriction rate in their study, while it was critical for assembling and initiating the bacterial divisome 24,25 .…”

Section: Resultssupporting

confidence: 87%

Progression of the late-stage divisome is unaffected by the depletion of the cytoplasmic FtsZ pool

Silber

Mayer

Opitz

et al. 2020

Preprint

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AbstractADEP antibiotics induce the degradation of the cell division protein FtsZ, thereby primarily depleting the cytoplasmic FtsZ pool that is needed for treadmilling FtsZ rings. We here studied the effect of ADEP on FtsZ ring formation. Our data reveal the disintegration of early FtsZ rings during ADEP treatment, while progressed FtsZ rings finalize cytokinesis, thus indicating different roles for FtsZ treadmilling during distinct stages of divisome assembly and constriction.

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Section: Resultssupporting

confidence: 87%

Progression of the late-stage divisome is unaffected by the depletion of the cytoplasmic FtsZ pool

Silber

Mayer

Opitz

et al. 2020

Preprint

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show abstract

Section: Resultssupporting

confidence: 92%

Progression of the late-stage divisome is unaffected by the depletion of the cytoplasmic FtsZ pool

et al. 2021

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Cell division is a central and essential process in most bacteria, and also due to its complexity and highly coordinated nature, it has emerged as a promising new antibiotic target pathway in recent years. We have previously shown that ADEP antibiotics preferably induce the degradation of the major cell division protein FtsZ, thereby primarily leading to a depletion of the cytoplasmic FtsZ pool that is needed for treadmilling FtsZ rings. To further investigate the physiological consequences of ADEP treatment, we here studied the effect of ADEP on the different stages of the FtsZ ring in rod-shaped bacteria. Our data reveal the disintegration of early FtsZ rings during ADEP treatment in Bacillus subtilis, indicating an essential role of the cytoplasmic FtsZ pool and thus FtsZ ring dynamics during initiation and maturation of the divisome. However, progressed FtsZ rings finalized cytokinesis once the septal peptidoglycan synthase PBP2b, a late-stage cell division protein, colocalized at the division site, thus implying that the concentration of the cytoplasmic FtsZ pool and FtsZ ring dynamics are less critical during the late stages of divisome assembly and progression.

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“…First is FtsZ pulling on the CM from the inside. This mechanism is supported by in vitro experiments showing that FtsZ alone can constrict liposomes (Osawa et al, 2008;Osawa and Erickson, 2013); also FtsZ is apparently needed to initiate constriction in vivo (Monteiro et al, 2018;Whitley et al, 2021). The second potential force mechanism is PGW synthesis pushing the CM from the outside.…”

Section: Discussionmentioning

confidence: 88%

How Teichoic Acids Could Support a Periplasm in Gram-Positive Bacteria, and Let Cell Division Cheat Turgor Pressure

Erickson

2021

Front. Microbiol.

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The cytoplasm of bacteria is maintained at a higher osmolality than the growth medium, which generates a turgor pressure. The cell membrane (CM) cannot support a large turgor, so there are two possibilities for transferring the pressure to the peptidoglycan cell wall (PGW): (1) the CM could be pressed directly against the PGW, or (2) the CM could be separated from the PGW by a periplasmic space that is isoosmotic with the cytoplasm. There is strong evidence for gram-negative bacteria that a periplasm exists and is isoosmotic with the cytoplasm. No comparable studies have been done for gram-positive bacteria. Here I suggest that a periplasmic space is probably essential in order for the periplasmic proteins to function, including especially the PBPs that remodel the peptidoglycan wall. I then present a semi-quantitative analysis of how teichoic acids could support a periplasm that is isoosmotic with the cytoplasm. The fixed anionic charge density of teichoic acids in the periplasm is ∼0.5 M, which would bring in ∼0.5 M Na+ neutralizing ions. This approximately balances the excess osmolality of the cytoplasm that would produce a turgor pressure of 19 atm. The 0.5 M fixed charge density is similar to that of proteoglycans in articular cartilage, suggesting a comparability ability to support pressure. An isoosmotic periplasm would be especially important for cell division, since it would allow CM constriction and PGW synthesis to avoid turgor pressure.

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FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation inBacillus subtiliscell division

Cited by 8 publications

References 54 publications

Progression of the late-stage divisome is unaffected by the depletion of the cytoplasmic FtsZ pool

Progression of the late-stage divisome is unaffected by the depletion of the cytoplasmic FtsZ pool

Progression of the late-stage divisome is unaffected by the depletion of the cytoplasmic FtsZ pool

How Teichoic Acids Could Support a Periplasm in Gram-Positive Bacteria, and Let Cell Division Cheat Turgor Pressure

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