Fucoidan induces Apoptosis in MDA-MB-231 Cells by Activating Caspase Cascade and Down-regulating XIAP

Banafa, Amal; Roshan, Sadia; Liu, Yunyi; Zhao, Shasha; Yang, Guangxiao; He, Guoqing; Chen, Mingjie

doi:10.9790/3008-09355964

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…The present study also detected the pathway of the fucoidan-induced apoptosis to be mitochondria-mediated, thus, confirming the LMWF-induced apoptosis to be via the activation of the intrinsic apoptosis pathway. This result is consistent with literature that the intrinsic pathway is the primary pathway in the fucoidan-induced apoptosis in the cancer cell lines [25,53].…”

Section: Resultssupporting

confidence: 93%

Fucoidan Extracted from the New Zealand Undaria pinnatifida—Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines

Shi

Chen

et al. 2018

Marine Drugs

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Fucoidan, the complex fucose-containing sulphated polysaccharide varies considerably in structure, composition, and bioactivity, depending on the source, species, seasonality, and extraction method. In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S). The five products differed in molecular composition. The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans. The difference of molecular compositions between S and S1 explained our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S. Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines—MCF-7 and MDA-MB-231. Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines. A time-dependent inhibition was only observed in the MCF-7. Induction of caspase-dependent apoptosis was observed in the MDA-MB-231 cells, through the intrinsic apoptosis pathway alone, or with the extrinsic pathway. LMWF stimulated a dose-dependent NOS activation in the MDA-MB-231 cells. In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines. Therefore, the LMWF from New Zealand U. pinnatifida could be used as a supplement cancer treatment.

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Section: Resultssupporting

confidence: 93%

Fucoidan Extracted from the New Zealand Undaria pinnatifida—Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines

Shi

Chen

et al. 2018

Marine Drugs

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“…However, the expression of XIAP in FLt + Dox treatment may regulate necroptosis [ 29 ], which needs to be confirmed in future investigations. Banafa et al showed that commercial fucoidan decreased the transcriptional expression of XIAP in MDA-MB 231 BC cells while increasing the expression of caspase 8, 3, and 9 [ 32 ]. Yang et al found that Undaria pinnatifida fucoidan downregulated XIAP expression and induced caspase 3 and 9 activation [ 33 ], as observed in this study.…”

Section: Resultsmentioning

confidence: 99%

Fucoidan from Lessonia trabeculata Induces Apoptosis through Caspase Dependent and Caspase-Independent Activation in 4T1 Breast Adenocarcinoma In Vitro

Cruz Riquelme,

Colona-Vallejos,

Alzamora-Gonzales

et al. 2024

Marine Drugs

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Experiments conducted on triple-negative breast cancer have shown that fucoidan from Lessonia trabeculata (FLt) exhibits cytotoxic and antitumor properties. However, further research is necessary to gain a complete understanding of its bioactivity and level of cytotoxicity. The cytotoxic effect of FLt was determined by the 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Apoptosis was analyzed using annexin V and caspase 3/7 staining kit and DNA fragmentation. In addition, transcriptional expression of antiapoptotic (Bcl-2 and XIAP) and proapoptotic (caspase 8, caspase 9, and AIF) genes were analyzed in TNBC 4T1 cells. After 72 h of culture, the IC50 for FLt was 561 μg/mL, while doxorubicin (Dox) had an IC50 of 0.04 μg/mL. In addition, assays for FLt + Dox were performed. Annexin V and caspase 3/7 revealed that FLt induces early and late-stage apoptosis. DNA fragmentation results support necrotic death of 4T1 cells. Similarly, transcripts that prevent cell death were decreased, while transcripts that promote cell death were increased. This study showed that FLt induces apoptosis by both caspase-dependent and caspase-independent mechanisms. These findings suggest that FLt may have potential applications in breast cancer treatment. Further research will provide more information to elucidate the mechanism of action of FLt.

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“…The total carbohydrate and uronic acid content were determined with the phenol-sulfuric acid [18] and carbazol-sulfuric acid [19] methods using L-rhamnose and D-glucuronic acid, respectively, as standards. The purified polysaccharide (70 mg) was dissolved in distilled water (20 mL) and sulfuric acid was added to reach a final concentration of 1.0 M. The mixture was subsequently heated to 100°C for 3 h. The resulting hydrolysate was neutralized with BaCO3.…”

Section: Chemical Proceduresmentioning

confidence: 99%

Induction of apoptosis by fucoidan isolated from a traditional food, Saccharina longissima, in U937 human leukemia cells

Shimabukuro

Yogi

Nago

et al. 2018

FFHD

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Background: A brown seaweed, Saccharina longissima (Laminaria angustata var. longissima) has been consumed in Okinawa for 300 years. It is native to the southernmost part of Japan, in Okinawa, but grows on the Pacific coast (Kushiro and Nemuro) in Hokkaido in the northernmost part of Japan. Previously, we reported the structural characteristics from S. longissima. The fucoidan consisted of L-fucose, D-galactose, D-glucose, D-xylose, D-glucuronic acid, and sulfuric acid. This fucoidan activated the RAW 264.7 murine macrophage cell line. The aim of this study was to investigate the effects of a fucoidan isolated from Saccharina longissima on cell anti-proliferation and apoptosis in the U937 human leukemia monocyte lymphoma cell line. Methods: U937 cells were incubated with four fucoidan concentrations. The degree of apoptosis was determined using the APOPercentage APOPtosis assay, caspaer-3 activity assay and Western blotting analysis assay. Results: The treatment of cell with a fucoidan highly substituted with sulfate, in addition to L-fucose residues, inhibited cell growth. Apoptosis increased in U937 cells in a dose-dependent manner following the addition of fucoidan. However, the fucoidan did not induce apoptosis in the presence of caspase inhibitor (z-VAD-FMK).Conclusions: The fucoidan from Saccharina longissima induced apoptosis in U937 cells. Accordingly, the kelp containing this fucoidan may contribute to the observed longevity of the Okinawan population.Keywords: fucoidan, Saccharina longissimi, brown seaweed, traditional food, human leukemia cells, apoptosis

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Fucoidan induces Apoptosis in MDA-MB-231 Cells by Activating Caspase Cascade and Down-regulating XIAP

Cited by 3 publications

References 30 publications

Fucoidan Extracted from the New Zealand Undaria pinnatifida—Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines

Fucoidan Extracted from the New Zealand Undaria pinnatifida—Physicochemical Comparison against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines

Fucoidan from Lessonia trabeculata Induces Apoptosis through Caspase Dependent and Caspase-Independent Activation in 4T1 Breast Adenocarcinoma In Vitro

Induction of apoptosis by fucoidan isolated from a traditional food, Saccharina longissima, in U937 human leukemia cells

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